Study on the kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid using capillary electrophoresis

Lu, Huang; Huang, Ying; Chi, Yuwu; Lin, Jin‐Ming; Yu, Lishuang; LiangJun, Xu

doi:10.1016/j.chroma.2007.10.078

Cited by 9 publications

(13 citation statements)

References 38 publications

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“…Our previous report [8] declared that good separation and shortened time was obtained when using b-CD as additive. Therefore, the CE behavior of tautomers in the existence of b-CD was examined in our study.…”

Section: Optimization Of Separation Conditionmentioning

confidence: 99%

“…Considering the migration time and peak shape, 6 mmol/L was chosen as the optimum concentration of b-CD. Based on the fact that b-CD played an important role in separation, inclusion complex between pHPP and b-CD was deduced, and their interaction has been explained by using electrochemical method [8]. In order to further confirm this interaction, UV spectrometry was applied to study the complex interaction between pHPP and b-CD in this article, and this will be discussed later in Section 3.4.…”

Section: Optimization Of Separation Conditionmentioning

confidence: 99%

“…As described in reference [1] and [8], there are two tautomers of pHPP, i.e. enolic and ketonic form in the aqueous solution, and the enolic form would convert spontaneously to the ketonic form in aqueous solution (Fig.…”

Section: Keto-enol Tautomerism Of Phppmentioning

confidence: 99%

“…Because of the physiological and clinical significance, chemistry of pHPP has been a topic of constant interest. The methods for determination of pHPP included flow injection spectrophotometry combined with single chip microcomputer [3], fluorescence quenching [4], GC [5][6], HPLC [7], CE [8][9][10], and electrochemical technique [1]. The Keto-enol tautomerism of pHPP was early investigated by Chi et al, using electrochemical method and UV spectrometry [1].…”

Section: Introductionmentioning

confidence: 99%

“…The Keto-enol tautomerism of pHPP was early investigated by Chi et al, using electrochemical method and UV spectrometry [1]. Recently, Chen and coworkers [8] studied keto-enol tautomerism of pHPP by using CE with UV detection.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Huang¹,

Zhang²,

LiangJun³

et al. 2009

J of Separation Science

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A high-performance CE with amperometric detection (CE-AD) was employed for the kinetic study of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP). Several factors (concentration of b-cyclodextrin (b-CD), concentration and pH of running buffer, separation voltage and injection time) affecting CE-AD were investigated and separation conditions were optimized. The kinetics of pHPP was performed in water solution and phosphate solution under different pH and temperature, the homologous ketonization rate constants and half-life were obtained. Also, the activation energy was calculated according to the rate constants under different temperature. The experimental results indicated that b-CD played an important role in the separation, therefore UV spectrometric method was applied for the study of complexation interaction between ketonic pHPP and b-CD. The results indicated that the stoichiometric ratio of the pHPP-b-CD complex was 1:1 and formation constant was determined. The obtained kinetic results are in correspondence with those reported by our group with CE-UV.

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Section: Optimization Of Separation Conditionmentioning

confidence: 99%

Section: Optimization Of Separation Conditionmentioning

confidence: 99%

Section: Keto-enol Tautomerism Of Phppmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Huang¹,

Zhang²,

LiangJun³

et al. 2009

J of Separation Science

Self Cite

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Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

Liu

Yang

et al. 2012

Luminescence

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A simple and new method for the simultaneous determination of procaine (Pro), lidocaine (Lid), ropivacaine (Rop) and bupivacaine (Bup) was developed using capillary electrophoresis separation with mixed micelles and electrochemiluminescence detection. The use of mixed micelles of 2.0 × 10(-3) mol/L sodium dodecyl sulfate (SDS) and 8.0 × 10(-3) mol/L Tween 20 greatly improved separation selectivity. The detection sensitivities of four drugs with a Pt working electrode were increased by modification of the Pt electrode with europium(III)-doped Prussian Blue analog (Eu-PB). Under optimal conditions, the four local anesthetics were well separated and detected. The limits of detection (LOD, S/N = 3) of Pro, Lid, Rop and Bup in standard solution are 2.5 × 10(-8) , 1.3 × 10(-8) , 3.0 × 10(-8) and 4.1 × 10(-8) mol/L, respectively. The limits of quantitation (LOQ, S/N = 10) of Pro, Lid, Rop and Bup are 2.3 × 10(-7) , 1.2 × 10(-7) , 3.7 × 10(-7) and 5.6 × 10(-7) mol/L in a human urine sample, and 8.5 × 10(-7) , 6.9 × 10(-7) , 2.8 × 10(-6) and 1.1 × 10(-6) mol/L in a human serum sample, respectively. The recoveries of four drugs at different spiked concentrations in human urine and serum samples were between 86.5 and 107.6%. The proposed method has been successfully applied to determine local anesthetics in biofluids.

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Tautomerism in pyridazin-3(2H)-one: A theoretical study using implicit/explicit solvation models

Emamian

Domingo

Tayyari

2014

Journal of Molecular Graphics and Modelling

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Study on the kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid using capillary electrophoresis

Cited by 9 publications

References 38 publications

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

Tautomerism in pyridazin-3(2H)-one: A theoretical study using implicit/explicit solvation models

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