Study on the Mechanism of Paeoniflorin Against Atherosclerosis Through Network Pharmacology and Molecular Docking

Zhao, Chengguo; Ling, Wenpei; Luo, Chunyu; Yin, Meifang; Qin, Shuzhi

doi:10.1166/jbmb.2020.1993

“…In recent two decades based on the development of systems biology, the concept of network pharmacology began to rise. The methods of omics data integration and multi-target drug development and related databases were applied Yang et al, 2020), especially in the research of traditional Chinese medicine (Zhao et al, 2020;Li et al, 2021;Zuo et al, 2021).…”

Section: O N L I N E F I R S T a R T I C L Ementioning

confidence: 99%

Anti-Stress Activity of Oviductus Ranae in Mice with Acute Stress Based on Network Pharmacology

Lin¹,

Li²,

Wang³

et al. 2023

PJZ

0

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Stress is a state of threatened homeostasis that causes the body to release the hormone cortisol produced by the adrenal glands. Oviductus ranae (OR) is an animal based raw material of folk medicine which plays a variety of activities. However, its anti-stress effects mechanism has not been fully revealed. In this work, based on network pharmacology, the potential targets of OR were screened, and an protein-protein interaction (PPI) network between the target of OR and anti-stress target was constructed using STRING database. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for analyzing the pathways of target gene. To further verify this, total 96 ICR mice were used, forced swim test and anoxic tolerance test were performed. The effect of OR on levels of monoamine neurotransmitters and phosphorylation of p38 which closely related to anti-stress were examined. The results showed that, 203 potential OR targets and 126 stress-related gene targets were obtained, in which there were 15 common targets. Pathway enrichment analysis showed that there were 20 critical pathways. The results revealed that OR could increase the total swimming time, increase the survival time of enduring anoxia, and regulate monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NE) and dopamine (DA). Western blot analyses indicated that OR may decrease the phosphorylation of p38. In conclusion, the results revealed that OR may play the anti-stress effects by inhibiting mitogen-activated protein kinase (MAPK) pathway, thus promote the normalization of acute stress. This study revealed the possible mechanism of OR as a potential material for the treatment of acute stress-related problems, and laid a foundation for the further development and utilization of OR.

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“…In recent two decades based on the development of systems biology, the concept of network pharmacology began to rise. The methods of omics data integration and multi-target drug development and related databases were applied Yang et al, 2020), especially in the research of traditional Chinese medicine (Zhao et al, 2020;Li et al, 2021;Zuo et al, 2021).…”

Section: O N L I N E F I R S T a R T I C L Ementioning

confidence: 99%

Immunomodulatory Effects of Calotropis procera on Human T-lymphocytes In Vitro

Batool¹

2023

PJZ

0

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Stress is a state of threatened homeostasis that causes the body to release the hormone cortisol produced by the adrenal glands. Oviductus ranae (OR) is an animal based raw material of folk medicine which plays a variety of activities. However, its anti-stress effects mechanism has not been fully revealed. In this work, based on network pharmacology, the potential targets of OR were screened, and an protein-protein interaction (PPI) network between the target of OR and anti-stress target was constructed using STRING database. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for analyzing the pathways of target gene. To further verify this, total 96 ICR mice were used, forced swim test and anoxic tolerance test were performed. The effect of OR on levels of monoamine neurotransmitters and phosphorylation of p38 which closely related to anti-stress were examined. The results showed that, 203 potential OR targets and 126 stress-related gene targets were obtained, in which there were 15 common targets. Pathway enrichment analysis showed that there were 20 critical pathways. The results revealed that OR could increase the total swimming time, increase the survival time of enduring anoxia, and regulate monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NE) and dopamine (DA). Western blot analyses indicated that OR may decrease the phosphorylation of p38. In conclusion, the results revealed that OR may play the anti-stress effects by inhibiting mitogen-activated protein kinase (MAPK) pathway, thus promote the normalization of acute stress. This study revealed the possible mechanism of OR as a potential material for the treatment of acute stress-related problems, and laid a foundation for the further development and utilization of OR.

show abstract

“…In recent two decades based on the development of systems biology, the concept of network pharmacology began to rise. The methods of omics data integration and multi-target drug development and related databases were applied Yang et al, 2020), especially in the research of traditional Chinese medicine (Zhao et al, 2020;Li et al, 2021;Zuo et al, 2021).…”

Section: O N L I N E F I R S T a R T I C L Ementioning

confidence: 99%

Effect of N-Acetylcysteine Oral Administration on Cutaneous Wound Healing

Sadaqat¹

2023

PJZ

1

0

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Stress is a state of threatened homeostasis that causes the body to release the hormone cortisol produced by the adrenal glands. Oviductus ranae (OR) is an animal based raw material of folk medicine which plays a variety of activities. However, its anti-stress effects mechanism has not been fully revealed. In this work, based on network pharmacology, the potential targets of OR were screened, and an protein-protein interaction (PPI) network between the target of OR and anti-stress target was constructed using STRING database. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for analyzing the pathways of target gene. To further verify this, total 96 ICR mice were used, forced swim test and anoxic tolerance test were performed. The effect of OR on levels of monoamine neurotransmitters and phosphorylation of p38 which closely related to anti-stress were examined. The results showed that, 203 potential OR targets and 126 stress-related gene targets were obtained, in which there were 15 common targets. Pathway enrichment analysis showed that there were 20 critical pathways. The results revealed that OR could increase the total swimming time, increase the survival time of enduring anoxia, and regulate monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NE) and dopamine (DA). Western blot analyses indicated that OR may decrease the phosphorylation of p38. In conclusion, the results revealed that OR may play the anti-stress effects by inhibiting mitogen-activated protein kinase (MAPK) pathway, thus promote the normalization of acute stress. This study revealed the possible mechanism of OR as a potential material for the treatment of acute stress-related problems, and laid a foundation for the further development and utilization of OR.

show abstract

Study on the Mechanism of Paeoniflorin Against Atherosclerosis Through Network Pharmacology and Molecular Docking

Cited by 4 publications

References 0 publications

Anti-Stress Activity of Oviductus Ranae in Mice with Acute Stress Based on Network Pharmacology

Anti-Stress Activity of Oviductus Ranae in Mice with Acute Stress Based on Network Pharmacology

Immunomodulatory Effects of Calotropis procera on Human T-lymphocytes In Vitro

Effect of N-Acetylcysteine Oral Administration on Cutaneous Wound Healing

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