Study on the mechanism of bone-mineral destruction by human monocytes.

Satō, Iwao; Iwakawa, Yoshinobu; Fugihashi, Hiroshi; Tatsumi, Junichi; Shimoyama, Masamichi; Marumoto, Yoshiko; Ikeda, Katumi

doi:10.2329/perio.29.530

Cited by 3 publications

(1 citation statement)

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“…Some macrophages were found to include aTCP particles by phagocytosis, while many macrophages adhered to aTCP, which would imply that those macrophages could affect aTCP by adhering to it. SATO et al (1987) have suggested in their study on the degradation of HA labeled with "Ca by human monocytes that HA might be dissolved in the specific areas to which human monocytes attach themselves, secreting acids in the process. The macrophages adhering to aTCP presumably degraded it in the same manner.…”

Section: Degradation Of Atcp By Macrophagesmentioning

confidence: 99%

An ultrastructural study of the process of hard tissue formation in amputated dental pulp dressed with .ALPHA.-tricalcium phosphate.

Koichi

Iwaku

Ozawa

1990

Arch. Histol. Cytol.

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This study was undertaken to determine the reaction of amputated dental pulp to the capping agent, alpha-tricalcium phosphate (alpha TCP), and to reveal the exact nature of the processes involved in both the healing of, and new hard tissue formation in the pulp when dressed with alpha TCP. The dental pulps of the anterior teeth of Macaca fuscata were dressed with alpha TCP ceramic 4-10 microns in diameter. The teeth were fixed by perfusion, then observed with light and electron microscopes. At 10 and 14 days postoperatively, the phagocytosis of the alpha TCP layer by the many macrophages and multinucleated giant cells, both of which had emerged beneath it, was seen. The synthesis of collagenous matrix underlying the alpha TCP layer began at 14 or 21 days, the cells involved exhibiting morphologic characteristics similar to those seen in osteoblasts. By the 90th day, only a bone-like hard tissue remained. No inflammation was observed in the residual dental pulp at any time during the experimental period. At 14 days, needle-like crystals were seen to have formed on the surface of alpha TCP particles, and by the 21st day, these crystals had extended to the newly synthesized matrix, with the result that calcification of the matrix was initiated, a calcified layer being superficially produced in the matrix. In the region of the matrix adjacent to the superficial calcified layer, a number of matrix vesicles were also found to function as another mechanism of matrix mineralization. Thus, it was concluded that the bone-like hard tissue was induced in the pulp capped with alpha TCP, and its potential for clinical application to exposed dental pulp was confirmed.

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Section: Degradation Of Atcp By Macrophagesmentioning

confidence: 99%

An ultrastructural study of the process of hard tissue formation in amputated dental pulp dressed with .ALPHA.-tricalcium phosphate.

Koichi

Iwaku

Ozawa

1990

Arch. Histol. Cytol.

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Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption

1988

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In this study, the effects of culture supernatants on various activities of the monocyte, as a bone-resorbing cell, were compared between peripheral blood leukocyte (PBL) cultures from patients with periodontal disease and those from subjects with a clinically healthy periodontium. We have reported that normal human monocytes in vitro induce the release of calcium from synthetic hydroxyapatite particles and that the activity is enhanced by supernatants from cultures of stimulated or non-stimulated peripheral blood leukocytes. Monocytes from both patients and healthy subjects induced the release of calcium from hydroxyapatite particles (HA) to an equal degree. This activity of monocytes from healthy subjects showed a statistically significant increase by addition of supernatants from stimulated or unstimulated cultures of peripheral blood leukocytes from periodontitis patients. This increase was greater than that seen with supernatants from cells of healthy controls. The Nitro Blue Tetrazolium reduction activity and [3H]-thymidine incorporation of monocytes were also increased by addition of the supernatants from leukocyte cultures from either patients or healthy controls, but no significant difference was noted in the increase. These results suggest that the HA-resorbing activity of monocytes was enhanced by factors from cultured leukocytes. Furthermore, these studies showed that production of these factors by peripheral mononuclear cells from patients with periodontal disease was greater than that seen with cells from normal subjects.

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The action of human peripheral monocytes on synthetic hydroxyapatite in vitro - Electron microscopic observations.

et al. 1988

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Study on the mechanism of bone-mineral destruction by human monocytes.

Cited by 3 publications

References 0 publications

An ultrastructural study of the process of hard tissue formation in amputated dental pulp dressed with .ALPHA.-tricalcium phosphate.

An ultrastructural study of the process of hard tissue formation in amputated dental pulp dressed with .ALPHA.-tricalcium phosphate.

Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption

The action of human peripheral monocytes on synthetic hydroxyapatite in vitro - Electron microscopic observations.

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