The interaction of SFN, an anticancer agent, with Aβ was studied using ESI-MS. SFN is found to bind covalently and specifically with the free NH(2) group of N-terminal aspartic acid and the ε-amino group of lysine at positions 16 and 28. Aggregation assay studies showed a lesser inclination of Aβ to aggregate when SFN is present. Hence the present study helps in understanding the mechanism of the action of SFN on the Aβ peptide.