Study on the purification and chemical compositions of tea glycoprotein

Nie, Shaoping; Xie, Mingyong; Fu, Zhaohui; Wan, Yiqun; Yan, Aiping

doi:10.1016/j.carbpol.2007.07.005

Cited by 75 publications

(35 citation statements)

References 17 publications

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“…27 The specific band at 1,042 cm -1 was due to the pyranose ring. 28,29 The wave number at 834 cm -1 represented the fingerprint region for α-glycosidic bond carbohydrate. 29 The peak at 701 cm -1 was attributed to N-H bending vibration.…”

Section: Ftir Analysismentioning

confidence: 99%

“…28,29 The wave number at 834 cm -1 represented the fingerprint region for α-glycosidic bond carbohydrate. 29 The peak at 701 cm -1 was attributed to N-H bending vibration. From these observations, it was thought that it was likely a glycoprotein containing polysaccharide with α-glycosidic bond and protein with β-sheet which capped on the AgNPs.…”

Section: Ftir Analysismentioning

confidence: 99%

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Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing

Lü¹,

Zeng²,

Zhang³

et al. 2016

IJN

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In this study, silver nanoparticles (AgNPs) were synthesized for the first time using an antibacterial endophytic fungus of Chinese medicinal herb Orchidantha chinensis , which has anti-inflammatory and antimicrobial activities. The AgNPs were analyzed by various characterization techniques to reveal their morphology, chemical composition, and stability. Also, the relationship between Chinese medicinal herbs, endophytic fungi, and the property of AgNPs was investigated for the first time. Interestingly, an experiment performed in this study revealed the proteins produced by the endophytic fungus to be capped on the nanoparticles, which led to an increase in the stability of spherical and polydispersed AgNPs with low aggregation for over 6 months. More importantly, further study demonstrated that the AgNPs possessed superior antibacterial activity and effectively promoted wound healing. Altogether, the biosynthesis of active AgNPs using the endophytic fungus from Chinese medicinal herb based on the symbiosis theory is simple, eco-friendly, and promising.

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Section: Ftir Analysismentioning

confidence: 99%

Section: Ftir Analysismentioning

confidence: 99%

Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing

Lü¹,

Zeng²,

Zhang³

et al. 2016

IJN

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“…Glycoprotein is a glycoconjugate in which a protein carries one or more carbohydrate chains covalently attached to a polypeptide backbone, usually via N-or O-linkages (Nie et al, 2008). It is reported that natural glycoproteins have a wide variety of biological functions, such as maintenance of protein conformation and stability, surface or intracellular recognition, cell adhesion, processing and diversion of protein, and so on.…”

Section: Introductionmentioning

confidence: 99%

Extraction Optimization, Preliminary Characterization and Antioxidant Activity of Glycoproteins from the Muscle of <i>Sepia pharaonis</i>

Dai

Sun

Zheng

et al. 2016

FSTR

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“…It can simplify the experiment and improve the purity of proteoglycan. At the same time, the operations of data collection were driven by UNICORN software, which guarantees quick, simple communications between systems and users and meets the stringent control and data handling procedures of modern laboratories [5].The yield of the crude water-soluble polysaccharide extracted with hot water from A. coreanum was 4.62%. The main fraction (ACP-I) was purified with DEAE-cellulose anion-exchange and Sepharose CL-6B gel filtration chromatography to yield 45% of the crude polysaccharide.…”

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confidence: 99%

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Purification and chemical compositions of a proteoglycan isolated from Aconitum coreanum

et al. 2010

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Proteoglycans represent a special class of glycoproteins that are heavily glycosylated. In nature proteoglycans have a wide variety of biological functions, such as maintenance of protein conformation and stability, immune and surface or intracellular recognition, inflammation, cell adhesion, pathogenicity, metastasis, and other cellular processes [1]. These functions may be dependent on either the protein part or the carbohydrate part, or on both.Aconitum coreanum (Levl.) Rapaics (Guanbaifu in Chinese) is one of the most documented centuries-old Chinese herbs in ancient Chinese medicinal literature. To date, A. coreanum has been used to treat various kinds of disorders such as cardialgia, facial distortion, epilepsia, migraine headache, vertigo, tetanus, infantile convulsion, and rheumatic arthralgia [2,3]. Some active components from A. coreanum, such as alkaloids and flavonol glycosides, have been widely investigated [4]. However, no specific studies on proteoglycan isolated from A. coreanum have been carried out. In this study a more refined water-soluble proteoglycan was isolated and purified, and some of its properties and chemical compositions were determined.In this study, an AKTA Explorer 100 purification system, equipped with a P-900 series pump, Monitor UV-900, Monitor pH/C-900, Fraction collector Frac-950, Auto-sampler A-900, and various kinds of columns, was employed to purify A. coreanum proteoglycan. It can simplify the experiment and improve the purity of proteoglycan. At the same time, the operations of data collection were driven by UNICORN software, which guarantees quick, simple communications between systems and users and meets the stringent control and data handling procedures of modern laboratories [5].The yield of the crude water-soluble polysaccharide extracted with hot water from A. coreanum was 4.62%. The main fraction (ACP-I) was purified with DEAE-cellulose anion-exchange and Sepharose CL-6B gel filtration chromatography to yield 45% of the crude polysaccharide.ACP-I appeared as a white powder, [ ] D 20 D +148q (c 0.2, H 2 O). The ACP-I was identified as consisting mainly of polysaccharide and protein by the phenol-sulfuric acid method and the Bradford method. As shown in Table 1, the percentages of polysaccharide and protein were 84.3% and 12.4%, respectively. ACP-I contained 20.3% uronic acid evaluated by the m-hydroxydiphenyl colorimetric method.The average molecular weight of ACP-I was calculated as 24 kDa by HPGPC according to the calibration curve with standard dextrans and glucose. The HPGPC profile also demonstrated that ACP-I had a single and symmetrically sharp peak, revealing that ACP-I was a homogeneous proteoglycan. GC analysis showed ACP-I was composed of five kinds of monosaccharides, namely fucose, arabinose, xylose, glucose, and galacturonic acid with molar ratios of 0.8:0.8:1:3.9:1.6. The results indicated Gal was the predominant monosaccharide.In the UV spectrum of the ACP-I, the intense peak at 209 nm was the characteristic band of double bonds or triple bonds. How...

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Study on the purification and chemical compositions of tea glycoprotein

Cited by 75 publications

References 17 publications

Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing

Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing

Extraction Optimization, Preliminary Characterization and Antioxidant Activity of Glycoproteins from the Muscle of <i>Sepia pharaonis</i>

Purification and chemical compositions of a proteoglycan isolated from Aconitum coreanum

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