Study on the Sleep-Improvement Effects of Hemerocallis citrina Baroni in Drosophila melanogaster and Targeted Screening to Identify Its Active Components and Mechanism

Abstract

Hemerocallis citrina Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep-improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in Drosophila melanogaster (D. melanogaster), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active constituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regulated the number of nighttime activities and total sleep time of D. melanogaster in a dose-dependent manner and positively regulated the sleep bouts and sleep duration of D. melanogaster. The target screening suggested that quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid were the main bioactive components of HC in sleep-improvements. Moreover, the core targets (Akt1, Cat, Ple, and Sod) affected by HC were verified by the expression of the mRNA of D. melanogaster. In summary, this study showed that HC could effectively regulate the sleep of D. melanogaster and further clarifies the multi-component and multi-target features of HC in sleep-improvement, which provides a new insight for the research and utilization of HC.

Effects of Dendropanax morbiferus Leaf Extract on Sleep Parameters in Invertebrate and Vertebrate Models

“…Our previous study reported that a mixture of 5-hydroxytryptophan, a precursor 5-HT, and GABA induces total sleeping time and increases NREM sleep in caffeine-mediated sleep loss in a vertebrate model linked to a Drosophila model [39]. Although exposure to 0.016 mM caffeine in a Drosophila mutant line lacking PGRP-LB with hyperactivation of NF-κB and 0.05% caffeine in wild type showed a positive effect on the endogenous antioxidant genes and enzymes, the number of nighttime activities and total sleep time were significantly reduced with decreased gene expression of SOD and CAT at 0.1% caffeine concentration [40,41].…”

Effect of Hemerocallis essence aroma on brain activity and sleep processes via scalp electroencephalogram

“…13 Daylily (Hemerocallis fulva) is a traditional Chinese plant used for food and medicine with sleep-promoting properties. [14][15][16] Daylily is known for its unique and distinctive floral scent, but these aroma characteristics have been lost over centuries of hybridization and selection. 17 And essential oils are difficult to extract, with very low yields ranging from 0.024% to 0.034%.…”

Polysaccharide from Hemerocallis citrina Borani by subcritical water with different temperatures and investigation of its physicochemical properties and antioxidant activity

“…Hemerocallis citrina Borani is rich in nutrition, which is a typical health vegetable with rich in protein, vitamins, and minerals. H. citrina Borani has been demonstrated multiple functions including antioxidant ( 2 ), antidepressant ( 3 , 4 ), lactation improving ( 5 ), and sleep promoting ( 6 ), as documented in the medicinal book “Compendium of Materia Medica” ( 7 ). The industry of H. citrina Borani is so far in the state of primary processing, with poor economic benefits and low value-added.…”