When operating, the CNC turning machine has to stay in good spindle bearing, accurately detecting wear of spindle bearing basically to procedure manual and time-consuming process. The CNC machine tool's spindle bearing vibration analysis has enabled timely detection of wear phenomena over time and local wear, unbalance in rotating, round-trip moving parts. Determining the time of adjustment and maintenance to restore preload is usually based on the wear of the spindle bearing in the normal working period. However, the time of preload adjustment and maintenance of spindle bearing can be based on the RMS (Root Mean Square) vibration index if the relationship of RMS vibration characteristics with total wear over time and limit wear allowed for spindle bearing are defined. This paper presents a research method to determine the relationship between the wear and RMS vibration characteristics of CNC lathe eclipse 300 spindles bearing under variable external load conditions. From there, determine the limit value of RMS vibration characteristics corresponding to the limit wear value of the spindle bearing of the CNC lathe and estimated time of adjustment Th for preload restore and maintenance.