Study on the Variation of the Distant Crossing Rice by Ion Beam Implantation

Abstract

In this paper, the following contents including the original receptor E1213 and other two control materials, RAPD polymorphism, photosynthetic efficiency, and the number of vascular bundles of the first internodes below the peduncle have been studied for the eight F7 transgenic lines obtained from ion beam implantation. The results showed that there was a significant variation in genomes of maize-rice line, compared with the receptor EI213, after the total exogenous maize DNA was introduced into EI213. The number of the vascular bundles of maize-rice progeny's lines was obviously much larger than those of the original receptor E1213 and other two controls GER-3 and MH63, and along with the photosynthetic efficiency of maize-rice progeny's lines being gone up. Moreover, the parenchyma cells around the vascular bundles of the transgenic lines became much larger in number and in size than those around the controls. All these indicated that the maize-rice progeny's lines are really different from and superior to the receptor and the controls. It is a novel and useful way to apply ion beam implantation in transferring DNA from Cq plant maize into C3 plant rice.

Effect of Ar+Implantation and Maize Genome DNA on Autotetraploid Rice

“…The experimental material was the Autotetraploid Rice with hereditary form stability induced from IR36, which is recorded as IR36 (4). Purple maize was provided by the Anhui Academy of Agricultural Sciences.…”

“…This method has attracted more attention . A great deal of research has been done by WU [4,5] , SONG [6] , WU [7] etc. The method and procedure of gene transfer induced by ion beams were also referenced [6] .…”

“…(4). (P < 0.01, n = 3) M: Marker, 1: purple maize, 2: IR36(4), 3: FM1, 4: FM2, 5: FM3 S5-3 is RAPD fragment amplified with a template of purple maize, FM2 and FM3 genome DNA.…”