Study the Antioxidant Effect of Tomato Extract in Oxidative Stressed Rats.

Abstract

Tomato, is today the most popular garden vegetable in worldwide, because of its high consumption. Tomato contain a variety of phytochemical , such as lycopene , flavonoids, glycosides and other chemical constituents that have been beneficial protective effect. The present study carried out to evaluate the effect of two different doses (2 mg/kg BWand 4 mg/kg BW) of tomato extract against hydrogen peroxide-induced oxidative stress in albino rats. Thirty two adult male albino rats randomly divided into four equal groups were used in this study, the results revealed that administration of 0.75% H 2 O 2 in drinking water (groupI) produced significant decline of antioxidant enzymes (superoxide dismutase (SOD) , glutathione (GSH), catalase (CAT)) and serum albumin concentration , with significant elevation of lipid peroxidation rate by estimation of malondialdehyde (MDA) and peroxynitrite radical (ONOO). Also, H 2 O 2 caused significant increase in serum concentration of alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and bilirubin. On the other hand the animals treated with H 2 O 2 plus 4 mg/kg BW of tomato extract (groupIII) showed significant increase of SOD, GSH, CATand albumin with significant reduction of MDA ,ONOO, ALT, AST, ALP, and bilirubin comparing with control group. Depending on the above oxidant and antioxidant markers, it seems that 4 mg/kg BW. of tomato extract exert beneficial action protect against H 2 O 2 induced oxidative stress in rats.

Estimation of some biochemical markers in acute myeloid leukemic patients before and after chemotherapy

“…The quantity of nitrophenol is equal to the quantity of peroxynitrite radical that is available in serum, and peroxynitrite (ONOO . ) radical estimation was based on the nitration of phenol via peroxynitrite radical, which resulted in the creation of nitrophenol, the absorption was recorded at 412 nm 20 .…”