Study the Effect of Aqueous Extract of Garlic (Allium sativum) on Healing Procedure of Burn Wound on Rat

Abstract

H IGH antimicrobial and antioxidant effects of garlic make it possible to use as an effective topical cream to heal burn wound injuries. The present research was done to evaluate the burn wound healing effects of the aqueous extract of garlic on rat model. Aqueous extract was achieved from fresh garlic. Thirty healthy adult rats were subjected to burn wounding and then randomly treated with silver sulfadiazine (positive control), cream base (negative control) and garlic topical cream (0.8%, 0.4% and 0.2%). Rats were then maintained for 21 days and macroscopic and microscopic examinations were performed through the experiment. Burn wound contraction was increased in all groups of rats during the experiment period. The highest percent of burn wound contraction at day 21 was found in rats treated with 0.4% garlic topical cream (88.10%), while those treated with cream base had the lowest wound contraction (70.30%). Rats treated with 0.4% garlic topical cream showed more rapid granulation, formation of scar and healingin the macroscopic examination. Additionally, rats treated with 0.4% garlic topical cream had the higher amounts of tissue granulation with dense collagen deposition in the microscopic examination after 21 days of wounding. Burn wound healing effects of the garlic extract was not dose depended. It seems that application of 4% garlic cream is an effective way to improve the burn wound healing of rats. However, supplementary studies are needed to evaluate other effects of garlic cream on burn wound.

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

“…Additionally, the morphometric assessment exhibited an increased wound contraction rate of the wounds following ASO and the Cytolcentella reference drug treatment as compared to the untreated group. Previous research using an aqueous extract of garlic ( Allium sativum ) showed a higher percentage of burn wound contraction in rats treated with 0.4% garlic topical cream (88.1%) than those treated with cream base (70.3%) [ 28 ]. This rapid wound closure may be attributed to an increase in fibroblast activity, which is crucial for normal wound contraction.…”

“…Wound care medicinal plants in the form of ointment could ameliorate wound healing and tissue repair with minimal side effects [ 27 ]. Previous studies reported that infections in the locations of injured skin are the main reasons for mortality in hospitalized patients with extensive burns [ 28 ]. For that reason, the application of plant medicinal preparations as local ointment with considerable antimicrobial effects can significantly reduce the risk of burn wound infections and alleviate the period of treatment [ 29 ].…”

In-vitro antibacterial activity of Kodai hill garlic (Allium sativum) aqueous extract against wound infection pathogens including MRSA

“…There are several reports of antibacterial activity of aqueous garlic extract (AGE) against a variety of wound infection causing pathogens [13][14][15][16][17] . Different studies reported that AGE exhibited activity against a large variety of Gram-positive and Gram-positive pathogenic bacteria including MDR strains [18] .…”

Electrospun PVA Nanofibers Loaded with Antimicrobial Herbal Extracts for Healing the Infectious Wound

“…The A. euchroma, A. sativum, and E. purpurea extracts possess antibacterial, anti-inflammatory, and wound-healing properties. However, their beneficial effects occurred after 14 days in mice and rat models (6,17).…”