Study the effect of static magnetic field on chromosomal aberrations on Vicia faba in area with high natural radioactivity

Abstract

This investigation was performed to evaluate the influence of the static magnetic field (SMF) on chromosomes in Vicia faba cultivated in soil from high background natural activity. Fourteen soil samples were collected from Ramsar area, in north of Iran, which the annual radiation absorbed dose from background radiation is substantially higher than the 20 mSv/year. The specific activity of the radionuclides of 232 Th, 236 Ra, and 40 K was measured using gamma spectrometry. The collected soil samples were then divided into 2 separate groups including HR (high natural radioactivity) and LR (low natural radioactivity). The cultivated Vicia faba in both groups were continuously exposed to SMF of 15 mT for 8 days, each 8 h/day starting from 2nd day. The chromosomal aberrations were counted in both groups with and without SMF from root of samples using chromosomal aberration method. The results showed a significant difference between treated and control plants. Chromosomal aberrations including bridge, fragments, and lagging chromosomes in prophase, metaphase, anaphase, and telophase enhanced significantly in treated groups compared with controls.

Comparison study of static and alternating magnetic field treatments on the quality preservation effect of cherry tomato at low temperature

“…3 | RESULTS AND DISCUSSION The SMF does not produce an extra electric field and thermal effect in the biological tissues. However, the non-thermal effect of the SMF is a very important factor that can cause the changing of a gene, cell, zymoprotein, and physicochemical property (Jouni, Abdolmaleki, Behmanesh, & Movahedin, 2014;Jouni, Abdolmaleki, & Ghanati, 2011).…”

Preliminary assessment of genotoxic effects induced by radiation from EAST using Vicia faba micronucleus assay

“…The MN assay was carried out using the process described in previous reports, with slight modifications [27][28][29]. After exposure for 5 d in the EAST hall during plasma discharge, the root tips of the V. faba seedlings were taken out and recovered in a thermostatic cabinet for another 24 h; then, the root tips (about 1 cm) were cut and fixed in Carnoy's solution (glacial acetic acid/ethanol 1:3 v/v) for about 4 h, then rinsed with deionized water for 10 min and stored in ethanol at 4 • C for slide preparation.…”

“…After exposure for 5 d in the EAST hall during plasma discharge, the root tips of the V. faba seedlings were taken out and recovered in a thermostatic cabinet for another 24 h; then, the root tips (about 1 cm) were cut and fixed in Carnoy's solution (glacial acetic acid/ethanol 1:3 v/v) for about 4 h, then rinsed with deionized water for 10 min and stored in ethanol at 4 • C for slide preparation. Afterward, the root tips were stained following the Feulgen technique, as previously described [29]. Schiff's reagent was used in this specific method for DNA staining because of the excellent contrast obtained (the chromosomes are magenta in color against a colorless cytoplasm).…”