Study the influence of culture conditions on rennin production by Rhizomucor miehei using solid-state fermentations

Aljammas, Houthail Alahmad; Fathi, Hassan Al; Alkhalaf, W.

doi:10.1016/j.jgeb.2017.10.004

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…Although no significant or positive effects of wheat bran supplementation on the feather meal medium were observed, preliminary studies performed in our research group (data not shown) demonstrated that the presence of wheat bran anticipates the peak production of the enzymes by A. niger LBA02, reducing the fermentation time required to achieve high levels of production. Several studies have reported the use of wheat bran as a suitable substrate for the production of enzymes by solid-state fermentation [11,[14][15][16].…”

Section: Resultsmentioning

confidence: 99%

Bioconversion of Chicken Feather Meal by Aspergillus niger: Simultaneous Enzymes Production Using a Cost-Effective Feedstock Under Solid State Fermentation

2019

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This study reported for the first time the simultaneous production of hydrolytic enzymes by Aspergillus niger under solid state fermentation using chicken feather meal as substrate. The effect of some culture parameters for production of protease, lipase, phytase and keratinase enzymes was evaluated using a central composite rotatable design. The results obtained demonstrated that the independent variables initial moisture of the culture medium and incubation temperature presented as highly significant on the enzymes production. The production of protease and lipase followed a similar profile, in which the highest values of enzymatic activities were detected after 48 h of fermentation. The conduction of the fermentative process using an initial moisture of 50%, 30 °C as incubation temperature and supplementation of the feather meal with 15% wheat bran resulted in higher yields of protease ([ 300 U g -1 ) and lipase ([ 90 U g -1 ) after 48 h and satisfactory values of phytase activity ([ 70 U g -1 ) after 72 h. No significant effects of the independent variables on keratinase production were observed. However, under the selected conditions for the other enzymes, keratinase production reached values higher than 13 U g -1 after 72 h fermentation. Thus, our work contributed to the proposal of an alternative process for the simultaneous production of proteases, lipases, phytases and keratinases in a single and simplified process using chicken feather meal.

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Section: Resultsmentioning

confidence: 99%

Bioconversion of Chicken Feather Meal by Aspergillus niger: Simultaneous Enzymes Production Using a Cost-Effective Feedstock Under Solid State Fermentation

2019

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“…The selection of mineral salts can significantly impact the microorganism's performance [9]. Differences in protease activity are influenced by variations in the chemical composition and particle size, which affect porosity [2].…”

Section: Determining the Optimal Moistening Solution Percentage For E...mentioning

confidence: 99%

“…These molds produce mucorpepsin (EC 3.4.23.23) and endothiapepsin (EC 3.4. 23.22) [9][10][11]. Acid proteases are widely used in the food industry, including applications such as cheese making, meat tenderization, protein analysis, and juice clarification.…”

Section: Introductionmentioning

confidence: 99%

Investigation of optimal parameters for aspartic protease production from Aspergillus terreus using solid-state fermentation

Hasnawi,

Abd Al-Manhel,

AL-Younis

2024

J App Biol Biotech

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The current study presents extracellular Aspartic protease production using low-cost agro-industrial waste with the indigenously isolated novel strain Aspergillus terreus, and parameters that largely affect enzyme production, namely fermentation time, pH, moistening solution, temperature, inoculum volume, and different solutions, were utilized for enzyme extraction. The isolated fungus was identified as A. terreus (registered as A. terreus SFH5 in the gene bank) by phenotypic and molecular characterization, showed the highest aspartic protease activity of 258.6 units/mL and 272.2 units/mg using a solid-state fermentation technique during screening, and was further used for media component screening and optimization studies. The study employed wheat bran as the preferred production medium and casein as the nitrogen source. The fermentation process was conducted at a temperature of 30°C and a pH of 5. The moistening solution to fermentation medium ratio was set at 3:1, while an inoculum volume of 2 mL (containing 1 × 106 spores/10 g solid) was used. Distilled water was selected as the most effective solution for extracting the enzyme. It has been discovered that the locally isolated novel strain of A. terreus, SFH5, can efficiently produce a substantial amount of aspartic protease by utilizing agro-industrial waste. This makes it a viable candidate for large-scale optimized production of the enzyme to satisfy industrial requirements.

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“…In nature, vegetables, animals and microorganisms, including bacteria and filamentous fungi are sources of proteolytic enzymes (Aljammas et al, 2018;Souza et al, 2019). The production of enzymes synthesized by microorganisms has acquired space in the market and industrial relevance, since proteases extracted from plants and animals are not supplying the commercial demand (Razzaq et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Lentinus Villosus Klotzsch (1833) Am 169: A Natural and Renewable Source of Alkaline Protease / Lentinus Villosus Klotzsch (1833) Am 169: Uma Fonte Natural E Renovável De Protease Alcalina

Braga¹,

Brito

Souza

et al. 2020

BJD

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Mushrooms have stood out as sources of proteases with industrial applicability. The objective of this work was to evaluate the parameters that influence the synthesis of proteases by Lentinus villosus Klotzsch (1833) AM 169 and to characterize these enzymes. The matrix culture, prepared on potato dextrose agar supplemented with yeast extract, was maintained for 8 days at 25 ° C. Submerged fermentation was carried out in GYPG (glucose, yeast extract, peptone and gelatin). Azocasein solution (1%, w/v) was used as an enzyme substrate. The best conditions for protease production were: initial pH of the culture medium (6), fermentation time (12 days), age of the inoculum (6 days) and size of the inoculum (10%). The proteases were active at pH 8, at 60 ° C, stable at pH (5 to 9) and temperature (30 °C to 60 °C). These enzymes were classified as cysteine proteases, stimulated by Fe 2+ and Mn 2+. Lentinus villosus synthesizes proteases with potential for application in the detergent, textile, leather processing and bioremediation processes.

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Study the influence of culture conditions on rennin production by Rhizomucor miehei using solid-state fermentations

Cited by 10 publications

References 12 publications

Bioconversion of Chicken Feather Meal by Aspergillus niger: Simultaneous Enzymes Production Using a Cost-Effective Feedstock Under Solid State Fermentation

Bioconversion of Chicken Feather Meal by Aspergillus niger: Simultaneous Enzymes Production Using a Cost-Effective Feedstock Under Solid State Fermentation

Investigation of optimal parameters for aspartic protease production from Aspergillus terreus using solid-state fermentation

Lentinus Villosus Klotzsch (1833) Am 169: A Natural and Renewable Source of Alkaline Protease / Lentinus Villosus Klotzsch (1833) Am 169: Uma Fonte Natural E Renovável De Protease Alcalina

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