Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers

Chokheli, Vasiliy; Shmaraeva, Antonina; Stepanenko, Viсtoriya; Kozlovsky, B. E.; Dmitriev, Pavel; Rajput, Vishnu D.; Azarov, Anatoly; Bakulin, Semyon; Varduni, Tatiana V.

doi:10.18522/2308-9709-2021-34-4

LaBS

2020

DOI: 10.18522/2308-9709-2021-34-4

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Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers

Vasiliy Chokheli¹,

Antonina Shmaraeva²,

Viсtoriya Stepanenko³

et al.

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Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

Zagorskaya

Abdurashytov

2021

IOP Conf. Ser.: Earth Environ. Sci.

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The species of the genus Mentha have been known since ancient times and have significant value in the pharmaceutical, cosmetic and food industries, as well as in medicine. For the widespread use of mint, including in a variety of breeding programs, and the preservation of genetic diversity, effective methods of maintaining cultivars and collection samples are required. Thanks to the development of biotechnological methods, in particular, the creation of slow-growing collections, are now actively used as an effective alternative to field collections. It is known that the cultivation of tissues and organs on artificial culture media can cause somaclonal variability. The purpose of this work is to study the effect of in vitro storage at 4-6°C without illumination after 3 and 4 in vitro conservation cycles on the genetic stability of three cultivars of mint Azhurnaya, Bergamotnaya and Zagrava using ISSR primers. 1 cycle: 1 year of in vitro conservation, microcutting and 2 subcultures of regrowth in a culture room. After conservation, the number of viable explants was 70.0-82.1%. Callus formation at the base of the shoots was not observed in any of the cultivars. After 3 and 4 cycles of in vitro conservation, genetic stability was assessed using 11 ISSR primers. It was found that all three mint genotypes showed full compliance (length and number of amplicons) with the profiles of control samples for all studied markers. It was also found that the markers used by ISSR are highly informative for mint cultivars.

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Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

Zagorskaya

Abdurashytov

2021

IOP Conf. Ser.: Earth Environ. Sci.

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show abstract

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Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers

Cited by 1 publication

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Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

Genetic identity of mint cultivars after in vitro conservation, assessed by ISSR primers

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