Study the Prevalence of Multidrug resistant bacteria from air samples collected from Mumbai & Suburban areas

Abstract

Introduction: Air comprises mixture of airborne bacteria & air pollutants in near surface of atmosphere. Air pollution is one of the major threat around the world due to change in physical and chemical parameters because of natural and anthropogenic activities; leading to climate change. Materials and Methods: A systematic analysis of AQI included air pollutants PM2.5, PM10, CO, SO2, NO2, O3, NH3 using SAMEER app developed by CPCB and meteorological parameters including wind speed and relative humanity. The overuse of antibiotics since last two decades has lead pathogenic bacteria gaining resistance. Results: The study was carried out to identify the prevalence of MDR bacteria in air from 8 different spatial variations of Mumbai & Suburban areas. 32 isolates from air were collected by settle plate technique. Enumeration and characterization of bacteria was performed. Antibiotic susceptibility test against 10 antibiotics; test showed that all bacterial isolates were sensitive to Tigecycline. The bacteria were highly resistant to Methicillin by 81.25 %. ARI of two isolates was 0.9 & MRI of Vasai, 0.625 was the highest. The prominent pollutant among 8 locations was found to be PM2.5, CO & PM10. PCA & hierarchical cluster analysis showed that MRI is not correlated to 8 parameters but, distinctly related to NH3. Conclusions: The MDR bacteria travel from soil, plants, water, humans, places, inanimate objects into the air. Hence, it is necessary to maintain the sanitation, ecological balance & usage of antibiotics when necessary.