Study to determine the presence of progenitor cells in the degenerated human cartilage endplates

Huang, Bo; Liu, Lantao; Li, Changqing; Zhuang, Yi; Luo, Gang; Hu, Shiyuan; Zhou, Yue

doi:10.1007/s00586-011-2039-4

Cited by 32 publications

(24 citation statements)

References 25 publications

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“…This similar promoting migration to repair (PMR) effect has also been observed in many other tissues [13][14][15][16]. Until now, stem cells derived from various parts of the IVD such as the nucleus pulposus (NP), annulus fibrosus (AF), and cartilage endplate (CEP) have been isolated and characterized [17][18][19][20], whereas little is known about endogenous stem cell migration with respect to difficulties in labeling target stem cells in vivo.…”

Section: Introductionmentioning

confidence: 94%

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

et al. 2015

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To the best of our knowledge, this is the first in vitro study aimed towards determining the existence and characteristics of ISN-SCs, which belong to the MSC family and with greater osteogenic and chondrogenic abilities than BMSCs according to our data. This finding may be of great significance for additional studies that investigate the migration of ISN-SCs into the IVD, and may provide a new perspective on different biological approaches for IVD self-regeneration.

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Section: Introductionmentioning

confidence: 94%

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

et al. 2015

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“…A previous study by our group identified stem cells in human degenerated CEPs, which were named as CEP stem cells (CESCs) ( 15 ). The present study investigated the effects of cyclic tensile stretch on the apoptosis of CESCs and investigated the underlying molecular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

BNIP3/Bcl‑2‑mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate‑derived stem cells

Yuan

et al. 2017

Exp Ther Med

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The present study aimed to investigate the molecular mechanisms of cyclic stretch-induced apoptosis in human intervertebral disc cartilage endplate-derived stem cells (CESCs). CESCs were stretched by the Flexercell-4000™ Tension Plus system, the effect on cell viability was measured by a Cell Counting Kit-8 assay, while cell apoptosis was detected by flow cytometry. Western blot analysis was used to evaluate the expression of B-cell lymphoma 2 (Bcl-2)/adenovirus E1B 19 kDa interacting protein 3 (BNIP3), Bcl-2, Bcl-2 homologous antagonist killer (Bak), Bcl-2-associated X protein (Bax), Bcl extra large (Bcl-xl) and the activity of caspase-3, while Z-VAD-FMK was used to inhibit caspase-3. Compared with the control group, the cell viability decreased in a time-dependent manner after stretching. Furthermore, cell apoptosis and the activity of caspase-3 were increased in a time-dependent manner. The ratio of pro-death factor BNIP3 to anti-apoptotic protein Bcl-2 was significantly increased. When cells were stretched for 36 h, the apoptosis-associated proteins Bak and Bax were increased, while Bcl-xl was decreased. The viability and apoptotic ratio of stretched cells were significantly restored after caspase-3 was repressed. In conclusion, cyclic tensile stretch induced apoptosis of CESCs, which was probably due to upregulation of the expression of BNIP3.

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“…In the present study, OIM was used under both hypoxic and normoxic conditions. Under normoxic conditions, Huang et al ( 34 ) reported that OIM was able to significantly induce the osteogenic differentiation of CESCs, compared with normal growth medium. It was observed in Huang et al ( 34 ) study that the expression of osteogenic differentiation marker genes (RUNX2, ALP and OC) was decreased in the absence of OIM under normoxic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Under normoxic conditions, Huang et al ( 34 ) reported that OIM was able to significantly induce the osteogenic differentiation of CESCs, compared with normal growth medium. It was observed in Huang et al ( 34 ) study that the expression of osteogenic differentiation marker genes (RUNX2, ALP and OC) was decreased in the absence of OIM under normoxic conditions. In addition, previous studies have demonstrated that stem cell differentiation may be decreased in hypoxic conditions compared with normoxic conditions ( 35 , 36 ).…”

Section: Discussionmentioning

confidence: 99%

A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells

Yao

Deng

Sun

et al. 2017

Molecular Medicine Reports

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It has been hypothesized that intervertebral disc degeneration is initiated by degeneration of the cartilage endplate (CEP), which is characterized by cartilage ossification. CEP-derived stem cells (CESCs), with the potential for chondro-osteogenic differentiation, may be responsible for the balance between chondrification and ossification in the CEP. The CEP remains in an avascular and hypoxic microenvironment; the present study observed that hypoxia was able to markedly inhibit the osteogenic differentiation of CESCs. This tissue-specific CESC differentiation in response to a hypoxic microenvironment was physiologically important for the prevention of ossification in the CEP. In order to study the hypoxia-regulated mechanisms underlying osteogenic differentiation of CESCs, a Human Transcriptome Array 2.0 was used to detect differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) during the osteogenic differentiation of CESCs under hypoxia, compared with those induced under normoxia. High-throughput analysis of DEGs and ASGs demonstrated that genes in the complement pathway were enriched, which may be a potential mechanism underlying hypoxia inhibition of CESCs osteogenesis. The results of the present study may provide a basis for future mechanistic studies regarding gene expression levels and alternative splicing events during the hypoxia-regulated inhibition of osteogenesis, which may be helpful in identifying targets for CEP degeneration therapy.

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Study to determine the presence of progenitor cells in the degenerated human cartilage endplates

Cited by 32 publications

References 25 publications

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

BNIP3/Bcl‑2‑mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate‑derived stem cells

A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells

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