Studying enzymatic bioreactions in a millisecond microfluidic flow mixer

Buchegger, Wolfgang; Haller, Anna; Driesche, Sander van den; Kraft, Martin; Lendl, Bernhard; Vellekoop, Michael J.

doi:10.1063/1.3665717

Cited by 26 publications

(14 citation statements)

References 36 publications

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“…Babich et al demonstrated the possibility of gram scale synthesis of phosphorylated compounds using phosphatase immobilized on Immobeads [34]. Buchegger et al used a microfluidic mixer to study the pre-steady state development of an enzymatic bioreaction and found that the dynamics of a biochemical reaction can be studied in a few seconds [35]. …”

Section: Introductionmentioning

confidence: 99%

Flow synthesis of phenylserine using threonine aldolase immobilized on Eupergit support

Tibhe¹,

Fu²,

Noël³

et al. 2013

Beilstein J. Org. Chem.

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Threonine aldolase (TA) from Thermotoga maritima was immobilized on an Eupergit support by both a direct and an indirect method. The incubation time for the direct immobilization method was optimized for the highest amount of enzyme on the support. By introducing the immobilized TA in a packed-bed microreactor, a flow synthesis of phenylserine was developed, and the effects of temperature and residence time were studied in particular. Calculations of the Damköhler number revealed that no mass transfer limitations are given in the micro-interstices of the packed bed. The yield does not exceed 40% and can be rationalized by the natural equilibrium as well as product inhibition which was experimentally proven. The flow synthesis with the immobilized enzyme was compared with the corresponding transformation conducted with the free enzyme. The product yield was further improved by operating under slug flow conditions which is related to the very short residence time distribution. In all cases 20% diastereomeric excess (de) and 99% enantiomeric excess (ee) were observed. A continuous run of the reactant solution was carried out for 10 hours in order to check enzyme stability at higher temperature. Stable operation was achieved at 20 minute residence time. Finally, the productivity of the reactor was calculated, extrapolated to parallel run units, and compared with data collected previously.2168

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Section: Introductionmentioning

confidence: 99%

Flow synthesis of phenylserine using threonine aldolase immobilized on Eupergit support

Tibhe¹,

Fu²,

Noël³

et al. 2013

Beilstein J. Org. Chem.

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“…5,6,13,[20][21][22][23] The technique uses co-flow of miscible focusing solution to confine a solution to a sub-cross-section of a channel. Groups develop various hydrodynamic focusing architectures for mitigating sticking to channel walls, 6 ensuring rapid mixing, 13,24,25 and ensuring uniform residence time among the focused solutes. 13,21,22 Three-dimensional (3D) hydrodynamic focusing sheaths a solution into a cylindrical core at the center of the channel, whereas twodimensional (2D) hydrodynamic focusing sheaths a solution into a columnar shape spanning the height of the channel.…”

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confidence: 99%

Analysis of a laminar-flow diffusional mixer for directed self-assembly of liposomes

et al. 2012

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The present work describes the operation and simulation of a microfluidic laminarflow mixer. Diffusive mixing takes place between a core solution containing lipids in ethanol and a sheath solution containing aqueous buffer, leading to self assembly of liposomes. Present device architecture hydrodynamically focuses the lipid solution into a cylindrical core positioned at the center of a microfluidic channel of 125 Â 125-lm 2 cross-section. Use of the device produces liposomes in the size range of 100-300 nm, with larger liposomes forming at greater ionic strength in the sheath solution and at lower lipid concentration in the core solution. Finite element simulations compute the concentration distributions of solutes at axial distances of greater than 100 channel widths. These simulations reduce computation time and enable computation at long axial distances by utilizing long hexahedral elements in the axial flow region and fine tetrahedral elements in the hydrodynamic focusing region. Present meshing technique is generally useful for simulation of long microfluidic channels and is fully implementable using COMSOL Multiphysics. Confocal microscopy provides experimental validation of the simulations using fluorescent solutions containing fluorescein or enhanced green fluorescent protein.

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“…Mixers in microfluidics can be classified into groups of passive and active, where the second type needs an external energy supply . Forces resulting from acoustic, dielectrophoretic, electromagnetic, electrohydrodynamic, temperature gradient, and pressure perturbations are examples of external energies used to improve mixing in active mixers …”

Section: Introductionmentioning

confidence: 99%

Numerical study of the effects of internal and external forces on the nanoparticle mixing in a ferrofluid flow

Hamdollahi

Aminfar

Mohammadpourfard

et al. 2019

Heat Trans. Asian Res.

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Micromixers are an essential part of microfluidic devices. Numerous studies have previously discussed improving the mixing efficiency in micromixers. In the present study, the scale analysis of different forces has been done to predict their influence on the behavior of the particles. Results show that the order of the magnitude of gravitational force is lower than other forces in this study and it is not effective. Then, in the absence of the magnetic field, the effects of different forces on the mixing of magnetic nanoparticles have been studied using the one‐way coupling method. According to the numerical results, it is observed that the Brownian force is essential for the normal behavior of nanoparticles. The thermophoretic force causes the penetration of particles into pure water, but homogeneous mixing does not occur. Finally, two methods have been introduced to improve mixing, including increasing the velocity of ferrofluid and magnetic field implementation. Both approaches make particle distribution more homogeneous. Incidentally, both of the above‐mentioned factors are useful for improving the mixing process. Using them together yields more homogeneous mixing in a shorter length of the mixing channel.

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Studying enzymatic bioreactions in a millisecond microfluidic flow mixer

Cited by 26 publications

References 36 publications

Flow synthesis of phenylserine using threonine aldolase immobilized on Eupergit support

Flow synthesis of phenylserine using threonine aldolase immobilized on Eupergit support

Analysis of a laminar-flow diffusional mixer for directed self-assembly of liposomes

Numerical study of the effects of internal and external forces on the nanoparticle mixing in a ferrofluid flow

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