Studying on genetic diversity and metabolic differences of Saccharomyces cerevisiae in Baijiu

Li, Qun; Lin, Bin; Tang, Jie; An, Longxu; Jiang, Wei; Li, Rui; Zhang, Gang; Yang, Qiang; Yang, Shengzhi; Chen, Shenxi

doi:10.1007/s00217-024-04489-w

Cited by 1 publication

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An amount of 50 mg of each biofilm sample was weighed accurately and added into 2 mL centrifuge tubes. Metabolites extraction and MS data collection were according to the previous method ( Li et al, 2024 ). Metabolomics data have been deposited to the EMBL-EBI MetaboLights database: with the identifier MTBLS9711.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effect of Lonicera japonica flos on Streptococcus mutans biofilm and mechanism exploration through metabolomic and transcriptomic analyses

Wang,

Liu,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionStreptococcus mutans was the primary pathogenic organism responsible for dental caries. Lonicera japonica flos (LJF) is a traditional herb in Asia and Europe and consumed as a tea beverage for thousands of years.MethodsThe inhibitory effect and mechanism of LJF on biofilm formation by S. mutans was investigated. The active extracts of LJF were validated for their inhibitory activity by examining changes in surface properties such as adherence, hydrophobicity, auto-aggregation abilities, and exopolysaccharides (EPS) production, including water-soluble glucan and water-insoluble glucan.Results and discussionLJF primarily inhibited biofilm formation through the reduction of EPS production, resulting in alterations in cell surface characteristics and growth retardation in biofilm formation cycles. Integrated transcriptomic and untargeted metabolomics analyses revealed that EPS production was modulated through two-component systems (TCS), quorum sensing (QS), and phosphotransferase system (PTS) pathways under LJF stress conditions. The sensing histidine kinase VicK was identified as an important target protein, as LJF caused its dysregulated expression and blocked the sensing of autoinducer II (AI-2). This led to the inhibition of response regulator transcriptional factors, down-regulated glycosyltransferase (Gtf) activity, and decreased production of water-insoluble glucans (WIG) and water-soluble glucans (WSG). This is the first exploration of the inhibitory effect and mechanism of LJF on S. mutans, providing a theoretical basis for the application of LJF in functional food, oral health care, and related areas.

show abstract

Section: Methodsmentioning

confidence: 99%