Studying Plant Secondary Metabolism in the Age of Genomics

Zhao, Nan; Wang, Guodong; Norris, Ayla; Chen, Xinlu; Chen, Feng

doi:10.1080/07352689.2013.789648

Cited by 51 publications

(26 citation statements)

References 103 publications

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“…The development of disease/ insect-resistant soybean may be assisted by the mechanistic elucidation of plant natural defences, especially the isolation of defence genes. The production of secondary metabolites is one strategy of plant natural defences against pathogens and insects (Bennett and Wallsgrove, 1994;Zhao et al, 2013). The most structurally diverse group of plant secondary metabolites is terpenoids, which have diverse roles in the interactions of plants with the environment, including serving as defences against pathogens and insects (Gershenzon and Dudareva, 2007).…”

Section: Introductionmentioning

confidence: 99%

An (E,E)‐α‐farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect‐induced volatiles

Lin

Wang

Chen

et al. 2016

Plant Biotechnology Journal

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SummaryPlant terpene synthase genes (TPSs) have roles in diverse biological processes. Here, we report the functional characterization of one member of the soybean TPS gene family, which was designated GmAFS. Recombinant GmAFS produced in Escherichia coli catalysed the formation of a sesquiterpene (E,E)‐α‐farnesene. GmAFS is closely related to (E,E)‐α‐farnesene synthase gene from apple, both phylogenetically and structurally. GmAFS was further investigated for its biological role in defence against nematodes and insects. Soybean cyst nematode (SCN) is the most important pathogen of soybean. The expression of GmAFS in a SCN‐resistant soybean was significantly induced by SCN infection compared with the control, whereas its expression in a SCN‐susceptible soybean was not changed by SCN infection. Transgenic hairy roots overexpressing GmAFS under the control of the CaMV 35S promoter were generated in an SCN‐susceptible soybean line. The transgenic lines showed significantly higher resistance to SCN, which indicates that GmAFS contributes to the resistance of soybean to SCN. In soybean leaves, the expression of GmAFS was found to be induced by Tetranychus urticae (two‐spotted spider mites). Exogenous application of methyl jasmonate to soybean plants also induced the expression of GmAFS in leaves. Using headspace collection combined with gas chromatography–mass spectrometry analysis, soybean plants that were infested with T. urticae were shown to emit a mixture of volatiles with (E,E)‐α‐farnesene as one of the most abundant constituents. In summary, this study showed that GmAFS has defence roles in both below‐ground and above‐ground organs of soybean against nematodes and insects, respectively.

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Section: Introductionmentioning

confidence: 99%

An (E,E)‐α‐farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect‐induced volatiles

Lin

Wang

Chen

et al. 2016

Plant Biotechnology Journal

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“…The advent of transcriptomic and metabolomic methods enables the exploration of complex metabolic and biosynthetic pathways that are central to host-pathogen interactions (Schauer and Fernie, 2006;Westermann et al, 2012;Zhao et al, 2013). In this article, we establish an attractive model system, using E. huxleyi as an ecologically important host and its lytic and nonlytic viruses.…”

Section: Introductionmentioning

confidence: 99%

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

et al. 2014

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Marine viruses are major ecological and evolutionary drivers of microbial food webs regulating the fate of carbon in the ocean. We combined transcriptomic and metabolomic analyses to explore the cellular pathways mediating the interaction between the bloom-forming coccolithophore Emiliania huxleyi and its specific coccolithoviruses (E. huxleyi virus [EhV]). We show that EhV induces profound transcriptome remodeling targeted toward fatty acid synthesis to support viral assembly. A metabolic shift toward production of viral-derived sphingolipids was detected during infection and coincided with downregulation of host de novo sphingolipid genes and induction of the viral-encoded homologous pathway. The depletion of host-specific sterols during lytic infection and their detection in purified virions revealed their novel role in viral life cycle. We identify an essential function of the mevalonate-isoprenoid branch of sterol biosynthesis during infection and propose its downregulation as an antiviral mechanism. We demonstrate how viral replication depends on the hijacking of host lipid metabolism during the chemical "arms race" in the ocean.

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“…However, a daunting task remains in making the link between a specific metabolite and a large number of candidate genes present in the databases (Schilmiller et al, 2012;Zhao et al, 2013). According to current models of gene evolution, specific genes of secondary metabolism evolved by duplication and subsequent functional specialization of pre-existing genes, e.g., those involved in primary metabolism (Conant and Wolfe, 2008;Freeling, 2014;Ober, 2005Ober, , 2010.…”

Section: Resultsmentioning

confidence: 99%

Single cell subtractive transcriptomics for identification of cell-specifically expressed candidate genes of pyrrolizidine alkaloid biosynthesis

et al. 2015

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Studying Plant Secondary Metabolism in the Age of Genomics

Cited by 51 publications

References 103 publications

An (E,E)‐α‐farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect‐induced volatiles

An (E,E)‐α‐farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect‐induced volatiles

Rewiring Host Lipid Metabolism by Large Viruses Determines the Fate of Emiliania huxleyi, a Bloom-Forming Alga in the Ocean

Single cell subtractive transcriptomics for identification of cell-specifically expressed candidate genes of pyrrolizidine alkaloid biosynthesis

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