Studying some cytotoxic and cytogenetic potentials of Dandelion methanolic extract on MCF-7 cancer cell line: an in vitro study

Al-Ezzy, Ruqaya M.; Al-Shanon, Ahmed F.; Khalaf, Hadeel Mohamed

doi:10.24996/ijs.2023.64.3.12

Cited by 4 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is need for precise diagnostic tools to guide treatment decisions [39]. Novel breast cancer treatment can help overcome resistance to therapy by inducing apoptosis in cancer cells [40,41] complexity of breast cancer [42,43]. Very recent studies have focused on the gene expression signature to sensitized breast cancer cells to particular chemotherapeutic agents such as Hesperetin [44].…”

Section: Discussionmentioning

confidence: 99%

The Effect of NF-κB Deactivation on Cancer Cell Response to ALA Mediated Photodynamic Therapy

Al-Khafaji,

Salman,

Hassan

et al. 2024

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Objective: Breast cancer is one of the most widespread tumors among women worldwide, which is difficult to treat due to the presence of chemoresistance and the risk of tumor recurrence and metastasis. There is a pressing necessity to develop efficient treatments to improve response for treatment and increase prolong survival of breast cancer patients. Photodynamic therapy (PDT) has attracted interest for its features as a noninvasive and relatively selective cancer treatment. This method relies on light-activated photosensitizers that, upon absorbing light, generate reactive oxygen species (ROS) with powerful cell-killing outcomes. Nuclear factor kappa B (NF-κB), a transcription factor, plays a key role in cancer development by regulating cell proliferation, differentiation, and survival. Inhibiting NF-κB can sensitize tumor cells to chemotherapeutic agents. Dimethyl fumarate (DMF), an NF-κB inhibitor approved by the FDA for multiple sclerosis treatment, has further shown promise in suppressing breast cancer cell growth in vitro. We hypothesized that combining PDT with Dimethyl fumarate (DMF) could further enhance therapeutic efficacy for both treatment modalities. Methods: In the current study, we explored the PDT effect of 1 and 2 mM aminolaevulinic acid (ALA) and low-power He-Ne laser irradiation combined with different concentrations of DMF (2.5, 1.25, or 0.652 µg/ml) against hormone nonresponsive AMJ13 breast cancer cell line that is derived from Iraqi patient. Results: Our results demonstrated that co-administration with all tested DMF concentrations significantly enhanced the cytotoxicity of PDT antitumor effect. The combination index analysis showed presence of synergism in combining PDT with DMF. Conclusion: This finding suggests that the combination of PDT with DMF could be a promising novel strategy against triple negative breast cancer that could be applied clinically due to the fact that both of these treatments are already clinically approved therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effect of NF-κB Deactivation on Cancer Cell Response to ALA Mediated Photodynamic Therapy

Al-Khafaji,

Salman,

Hassan

et al. 2024

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

show abstract

“…[9][10][11] Furthermore, several earlier investigations have demonstrated the anti-tumor potential of different dandelion extracts. [12][13][14][15] It has been reported in various studies that ethanol extract has a higher content of bioactive molecules than water extract. 16 Although the cytotoxicity of the water extract from the dried dandelion root on the pancreatic cancer cell line has already been established, 11 no research has been encountered in the literature about the cytotoxic impact of the ethanol extract made from the fresh dandelion root, which contains more bioactive molecules, on the PANC-1 cancer cell line.…”

Section: Introductionmentioning

confidence: 99%

In vitro Cytotoxicity Evaluation of Dandelion Root Ethanol Extract on PANC-1 Cell Line

Aras,

Arslan,

Özçay

2024

MMJKK

View full text Add to dashboard Cite

A challenging diagnosis is the outcome of pancreatic cancer's aggressivity and malignant character. This type of cancer is mostly detected in the metastatic stage. Current treatment options have various side effects, result in the survival rates and quality of life of patients are extremely decreased. Dandelion (Taraxacum officinale (L.) Weber ex F.H. Wigg.) is one of the natural products that has the potential to be therapeutic against a wide range of diseases. This study aimed to evaluate the potential of ethanol extract of dandelion root (DRE) as an alternative anticancer agent. Based on this purpose, milk-rich dandelion roots were collected, cut into small pieces, and then extracted using Soxhlet in the presence of 70% ethanol. The cytotoxic effect of the DRE at certain doses (10, 5, and 2.5 mg/mL) was determined by the Microtetrazolium (MTT) assay for 24, 48, and 72 hours, and half-maximal inhibitory concentration (IC)50 values for these incubation periods were found to be 10 mg/mL, 6.80 mg/mL and 6 mg/mL (p0.001), respectively. As a result, DRE may reduce cell viability when applied to aggressive pancreatic cancer cells and may have the potential to be an alternative anticancer agent. This potential can be clarified with further studies.

show abstract

“…Studies have shown that the effects of Mannich based on the growth of MCF-7 cells were found to inhibit cell growth in a dosedependent manner. Other research reported that Mannich bases could induce apoptosis in MCF-7 cells [7]. Another study investigated the effects of Mannich bases on the expression of genes involved in the growth and survival of MCF-7 cells.…”

Section: ■ Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Evaluation of MCF-7 (Breast Cancer) for Schiff, Mannich Bases, and Their Complexes

Al-Khazraji

2024

Indones. J. Chem.

View full text Add to dashboard Cite

A new synthesis of Schiff (K) 6 and Mannich bases (Q) 7 had formed compound (Q) 7 by reacting compound (K) with N-methylaniline at the presence of formalin 35% to given Mannich base (Q). Additionally, new complexes were formed by reacting Schiff base (K) with metal salts CuCl2·2H2O, PdCl2·2H2O, and PtCl6·6H2O by 2:1 of M:L ratio. New ligands and their complexes were characterized, exanimated, and confirmed through several techniques, including FTIR, UV-visible, 1H-NMR, 13C-NMR spectroscopy, CHN analysis, FAA, TG, molar conductivity, and magnetic susceptibility. These compounds and their complexes were screened against breast cancer cells. It was determined that several of these compounds had a significant anti-breast cancer effect due to their ability to induce cell death and reduce tumor growth. Metal complexes have been thoroughly studied as a potential anti-cancer therapy and have shown promising results. It was revealed these complexes uniquely affect the biological processes involved in cancer growth, leading to apoptosis and fading of cells. To form MCF-7 cell cultures, MCF-7 cells were seeded in 96-well plates at an appropriate density (5 × 105 cells/well). Metal complexes utilized in MCF-7 therapy have the potential to disband some of the harms associated with chemotherapy, such as drug resistance and toxicity.

show abstract

Studying some cytotoxic and cytogenetic potentials of Dandelion methanolic extract on MCF-7 cancer cell line: an in vitro study

Cited by 4 publications

References 16 publications

The Effect of NF-κB Deactivation on Cancer Cell Response to ALA Mediated Photodynamic Therapy

The Effect of NF-κB Deactivation on Cancer Cell Response to ALA Mediated Photodynamic Therapy

In vitro Cytotoxicity Evaluation of Dandelion Root Ethanol Extract on PANC-1 Cell Line

Synthesis, Characterization, and Evaluation of MCF-7 (Breast Cancer) for Schiff, Mannich Bases, and Their Complexes

Contact Info

Product

Resources

About