Studying the effect of g-irradiated celery leaves on antioxidant status and cardiac enzymes in hypercholesterolemic rats

Shahat, A.N. El; Azeem, Amir; Mekawey, H. M. S.; El-megid, Mohamed H.M. Abd

doi:10.18805/ijar.v0iof.9184

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…SOD is a metal enzyme that catalyzes the dismutation of superoxide radicals. CAT is a heme protein that catalyses the decrease of H 2 O 2 to H 2 O and O 2 , therefore protects cells from the oxidative damage of H 2 O 2 and OH - (Shahat et al, 2017). GSH-Px is a seleno enzyme.…”

Section: Effects On Enzymatic Antioxidantsmentioning

confidence: 99%

Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats

Wang

Zhu

Cai

et al. 2018

IJAR

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Cadmium (Cd)-induced oxidative damage of liver and kidney and the ameliorative impact of baicalein against hepatotoxicity and nephrotoxicity of rats was studied. Male SD rats were handled with either intraperitoneal cadmium (CdCl2, 2mg/kg) and/or oral baicalein (100 mg/kg) for 4 weeks. The results demonstrated that Cd increased the level of GPT, GOT, BUN in serum and the contents of MDA, GSH, decreased the activities of SOD, CAT, GSH-Px in tissues versus control. Conversely, administration of baicalein restored the changes in these parameters to nearly normal levels. The results suggested that the ameliorative impact of baicalein might be because of its antioxidant properties in combating free radical-induced oxidative stress resulting from cadmium chloride.

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Section: Effects On Enzymatic Antioxidantsmentioning

confidence: 99%

Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats

Wang

Zhu

Cai

et al. 2018

IJAR

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“…We payed attention to Nrf2/HO‐1 and it has been proofed that activation of Nrf2 can upgrade its the downstream targets HO‐1 (Ruiz, Pergola, Zager, & Vaziri, ). Similarly, GST activity in spleen was upgraded resulting in increasing the peroxidase activity of GSH‐PX, as well as a reduction on ROS and MDA level, consist with results in other experimental models (El Shahat, Azeem, Mekawey, & Abdel‐Megid, ). For example, celery a protective effect on the liver via it's antioxidant action against the toxicity included by precursor compounds (Sukketsiri et al, ).…”

Section: Resultsmentioning

confidence: 99%

Effect of digestion on the phenolic content and antioxidant activity of celery leaf and the antioxidant mechanism via Nrf2/HO‐1 signaling pathways against Dexamethasone

et al. 2019

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The effect of digestion on the phenolic compounds and antioxidant activity of celery leaf were performed. In this work, 13 phenolic chemicals were discriminated by HPLC‐MS, and content of phenolic and the antioxidant capacity were evaluated after digestion in vitro. After digestion, the content of phenols and flavonoids were increased by about 3–6‐folds correlated with the average antioxidant activity (p < 0.05). It was found that the extraction of celery leaf (ET) decreased lipid peroxidation (MDA) and reactive oxygen species (ROS) level, and elevated the antioxidant activities of the liver, spleen, and thymus in Dexamethasone (Dex)‐treated KM mice. Furthermore, ET increased the protein transcription of NF‐E2‐related factor 2 (Nrf2), hemeoxygenase‐1 (HO‐1) and glutathione s‐transferase (GST) to against oxidation. These results suggested that ET can protect animals through the Nrf2/HO‐1 signaling pathway from oxidative damage included by Dex. Practical applications Celery is a daily edible vegetable with more pharmacological research focused on dietary fiber, yet fewer studies on the biological activity of small molecules, especially that in leaves. This study shows that the phenolic compounds from celery leaf have a distinct enhancement of oxidation after digestion in vitro, and the celery leaf reduces oxidative stress induced by Dex via Nrf2/HO‐1 signaling pathway, indicating celery leaf or other food rich in phenolic compounds can be good source of functional food to fully use to promote the economic value. Moreover, it also provides theoretical information of celery leaf on digestion, which insinuates that food or Chinese medicine containing flavonoids, such as glycoside of apigenin or luteolin, have the similar digestion pattern, providing theoretical basis for later metabolism. Therefore, the absorption and metabolism of ET or flavonoids after digestion in body and the upstream signaling pathway activating Nrf2/HO‐1, like PI3K or JNK phosphorylation, or downstream signaling pathway need further research.

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“…In the middle and late periods of fermentation, the flavonoids originating from the seeds dissolved into the broth, due to the increase in ethanol concentration [29] (the detailed data for ethanol content are shown in Figure S1 in the Supplementary Materials). In addition, with the assistance of specific enzymes, a portion of other carbon sources could be converted into compounds with the structure of pyrocatechol during microbial metabolism [33], which also contributed to the increase in flavonoid content as determined by colorimetry.…”

Section: Changes In Flavonoid Content During Fermentationmentioning

confidence: 99%

Improved Antioxidant Capacity of Akebia trifoliata Fruit Inoculated Fermentation by Plantilactobacillus plantarum, Mechanism of Anti-Oxidative Stress through Network Pharmacology, Molecular Docking and Experiment Validation by HepG2 Cells

et al. 2023

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In this work, spontaneously fermented and inoculation-fermented Akebia trifoliata fruit Jiaosu (SFAJ/IFAJ) were compared. The key metabolites and antioxidant activities of SFAJ and IFAJ were tracked and tested during fermentation. The antioxidant effect of fermented Akebia trifoliata fruit and the underlying mechanisms were explored using network pharmacology for the prediction and verification of the molecular targets and pathways of the Akebia trifoliata fruit’s action against oxidative stress. Furthermore, the results were verified by molecular docking and then investigated, based on a HepG2 cell model. The results of correlation analysis and principal component analysis (PCA) showed that there were significant positive correlations between the phenols, flavonoids, and terpenoids in SFAJ and IFAJ and their antioxidant activities. Network pharmacology and molecular docking analysis disclosed the antioxidation mechanism at the molecular level. In addition, both SFAJ and IFAJ were effective at alleviating oxidative stress in HepG2 cells. In particular, IFAJ performed better than SFAJ in protecting cells with an intracellular reactive oxygen species (ROS) level of 99.96 ± 4.07 U/mg prot, superoxide dismutase (SOD) activity of 41.56 ± 0.06 U/mg prot, catalase (CAT) activity of 91.78 ± 3.85 U/mg prot, and glutathione peroxidase (GSH-Px) activity of 39.32 ± 2.75 mU/mg prot in the IFAJ group. Collectively, this study revealed the changes in bioactive metabolite contents and the in vitro antioxidant activity during fermentation and investigated the protectiveness of SFAJ and IFAJ against oxidative stress within HepG2 cells, promoting the study of the antioxidant efficacy of IFAJ, thereby providing valuable reference data for the optimization of its preparation and the development of relevant products with health benefits.

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Studying the effect of g-irradiated celery leaves on antioxidant status and cardiac enzymes in hypercholesterolemic rats

Cited by 5 publications

References 20 publications

Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats

Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats

Effect of digestion on the phenolic content and antioxidant activity of celery leaf and the antioxidant mechanism via Nrf2/HO‐1 signaling pathways against Dexamethasone

Improved Antioxidant Capacity of Akebia trifoliata Fruit Inoculated Fermentation by Plantilactobacillus plantarum, Mechanism of Anti-Oxidative Stress through Network Pharmacology, Molecular Docking and Experiment Validation by HepG2 Cells

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