Flow visualizations have been performed on a free flying, flapping-wing micro air vehicle (MAV), using a large-scale particle image velocimetry (PIV) approach. The PIV method involves the use of helium-filled soap bubbles (HFSB) as tracer particles. HFSB scatter light with much higher intensity than regular seeding particles, comparable to that reflected off the flexible flapping wings. This enables flow field visualization to be achieved close to the flapping wings, in contrast to previous PIV experiments with regular seeding. Unlike previous tethered wind tunnel measurements, in which the vehicle is fixed relative to the measurement setup, the MAV is now flown through the measurement area. In this way, the experiment captures the flow field of the MAV in free flight, allowing the true nature of the flow representative of actual flight to be appreciated. Measurements were performed for two different orientations of the light sheet with respect to the flight direction. In the first configuration, the light sheet is parallel to the flight direction, and visualizes a streamwise plane that intersects the MAV wings at a specific spanwise position. In the second configuration, the illumination plane is normal to the flight direction, and visualizes the flow as the MAV passes through the light sheet.Aerospace 2018, 5, 99 2 of 15 Quite a variety of visualization PIV studies on animals in free flight have been reported, see e.g., [11][12][13]; however, the direct correspondence to the current MAV configuration is limited. Firstly, most of these studies consider single-wing configurations at relatively fast forward flight, for which the flow dynamics are less complex than for hovering or slow forward flight, especially in the case of counter-flapping wing configurations with large-amplitude and high-frequency stroke behavior, as considered here. Furthermore, flow visualizations reported in literature have predominantly been limited to wake visualizations, while near-wing visualizations suffer in large regions around the wing from reflections or the obstruction of the illumination [13].The MAV used in the current tests is the DelFly II (henceforth called DelFly for simplicity) [3], for which tethered experiments have been previously performed in hovering [14,15] and symmetric forward flight (at zero pitch angle) [9] configurations. These configurations both result in symmetrical flow patterns around the wings, which is not representative of true forward flight, where an appreciable forward velocity is combined with a high pitch angle. This will result in upper and lower wings experiencing distinctly different aerodynamic conditions. Tethered flow visualization experiments have been carried out on a different DelFly version at relatively small pitch angles [16], whereas [17] reports on preliminary results of a setup intend to enable free-flight wake visualization by controlling the MAV at a fixed position in the exit of a large wind tunnel. The current investigation takes a different and novel approach by aiming to visualiz...