Studying the efficacy of antiplatelet drugs on atherosclerosis by optofluidic imaging on a chip

Abstract

Vascular stenosis caused by atherosclerosis instigates activation and aggregation of platelets, eventually resulting in thrombus formation. Although antiplatelet drugs are commonly used to inhibit platelet activation and aggregation, they unfortunately...

Deciphering the efficacy of antiplatelet drugs under vascular stenosis by high-speed on-chip optofluidic imaging

“…Each blood sample was then perfused through the stenosis chip by a high-flow-rate peristaltic pump (P720/66, Instech Laboratories) at a flow rate of 3.0 mL/min in a closed perfusion loop for a 10-min interval. Next, platelets were extracted from perfused blood samples using density-gradient centrifugation (Lymphoprep, STEMCELL Technologies, #07801), followed by immunofluorescent probe labeling (PE anti-human CD61 antibody (BioLegend, 336405) and 2% paraformaldehyde fixation [6][7][8]. All blood samples were processed within 3 h. All the experiments were repeated three times with each condition.…”

Advancing precision single-cell analysis of red blood cells through semi-supervised deep learning using database of patients with post-COVID-19 syndrome

“…Future studies could further dissect the morphological differences among platelet aggregates to find additional markers that predict the disease progression or treatment response, especially in patients with viral infections (COVID- 19), where excessive platelet aggregation is linked to the disease severity 28,40 . Additionally, analyzing platelet morphology could reveal key features linked to function or dysfunction, paving the way for personalized treatments in platelet disorders 9,28 .…”

Microfluidic-based cardiovascular systems for advanced study of atherosclerosis

“…124 Deng et al developed an in vitro 3D stenosis microfluidic system to simulate the atherogenic flow environment of vascular stenosis, evaluating the efficacy of three antiplatelet drugs (acetylsalicylic acid, cangrelor, and eptifibatide) in inhibiting stenosis-induced platelet aggregation. 125 The experiment showed that these drugs have varying abilities to inhibit platelet aggregation caused by stenosis, with acetylsalicylic acid, cangrelor, and eptifibatide exhibiting increasing effectiveness. This method provides a means to evaluate the efficacy of antiplatelet drugs in vascular stenosis and aids in determining safe and effective treatments for AS patients.…”