Abstract-This paper reports the result of study on development, implementation and evaluation of animation construction for integrated science teaching. The method of the study was research and development (R&D) consisted of two years research. The first year study was the development of animation resulted in topics of environment, energy, exploding candy and movement animations. In the second year study, the animation was implemented in three classrooms involving students from second year of secondary schools. Evaluation addressed by knowledge test and students' questionnaire. The knowledge test resulted in a value of N gain 0.43 mean that the improvement of students' understanding of science concept in a medium category. Weak experiment resulted in t score 2.926 showed the fulfillment of mastery learning. Furthermore, student opinions about the animation were positive