Studying the kinetic of midodrine degradations using TLC stability approach: Application to dosage form and human plasma

Abdelgaleel, Mahmoud; Nagi, Dalia M.; Derayea, Sayed M.; Khashaba, Pakinaz Y.

doi:10.1016/j.jpba.2023.115322

Cited by 4 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In chromatographic methods, confirmation of identity is obtained by comparing the retention times or retardation factors (TLC) of the test substance and the reference substance [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug, impurities present in the drug, but also for investigating many physicochemical properties, including lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

View full text Add to dashboard Cite

An easy and short-time TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyzes were performed on chromatographic plates precoated with silica gel 60F254 using the optimal mobile phase: chloroform + methanol + diethylamine in a volume ratio of 9:1:1. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopoeial method. The presented method is fast, sensitive, precise, selective, accurate and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

show abstract

Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The most commonly used methods for examining the identity of drugs are infrared absorption spectrophotometry, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas chromatography (GC) [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug and any impurities present in the drug, as well as for investigating many physicochemical properties, including the lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

Pyka-Pająk

2024

Processes

View full text Add to dashboard Cite

A sensitive, easy-to-use, fast, and cost-effective TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyses were performed on chromatographic plates precoated with silica gel 60F254 using chloroform + methanol + diethylamine in a volume ratio of 9:1:1 as the optimal mobile phase. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopeial method. The presented method is fast, sensitive, precise, selective, accurate, and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

show abstract

Greenness and practicality assessment of innovative TLC approach for facile determination of hepatorenal syndrome combination therapy “octreotide and midodrine”: Application to pharmaceuticals, plasma, and urine

Attia,

Abdulrazik,

Derayea

2025

Journal of Molecular Liquids

View full text Add to dashboard Cite

Studying the kinetic of midodrine degradations using TLC stability approach: Application to dosage form and human plasma

Cited by 4 publications

References 23 publications

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

Greenness and practicality assessment of innovative TLC approach for facile determination of hepatorenal syndrome combination therapy “octreotide and midodrine”: Application to pharmaceuticals, plasma, and urine

Contact Info

Product

Resources

About