Studying the MicroRNA role as a survival predictor and revealing its part in malignancy level determination in patients with supratentorial gliomas of brain

Stupak, E. V.; Veryaskina, Yu. A.; Титов, С. Е.; Achmerova, L. G.; Ступак, В. В.; Долженко, Д. А.; Rabinovich, S. S.; Narodov, A. A.; Иванов, М. Ф.; Жимулев, И. Ф.; Kolesnikov, N. N.

doi:10.1063/1.5001652

Cited by 1 publication

(5 citation statements)

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“…Its increased expression is observed in glioma tissues of the brain [23]. A statistically significant increase in the level of this miRNA in gliomas of high malignancy degree (grade III-IV) compared with normal brain tissues was noted in our study [18]. This work shows that increased miRNA-221 expression (HR = 0.814; 95%CI 0.683-0.970; p < 0.0216) significantly correlates with a decrease in the survival of operated patients.…”

Section: Discussionsupporting

confidence: 69%

“…Its increased expression is observed in brain glioma tissues [23]. A statistically significant increase in the level of this miRNA in gliomas of high malignancy degree (grade III-IV) compared with normal brain tissues was noted in our study [18]. MiRNA -221 is oncogenic, it targets the p57 protein, and the TIMP3 gene regulates the cell cycle and cell survival processes [22].…”

Section: Discussionmentioning

confidence: 73%

“…Our previous study and the study by Silber et al have shown that the miRNA-124 level is statistically significantly reduced in gliomas, including glioblastomas, compared to the tumor-adjacent tissue [18,28]. This miRNA regulates the cell cycle at G0/G1 and inhibits the CDK6 kinase, which stimulates angiogenesis [19,28], leading to the formation of new vessels.…”

Section: Discussionmentioning

confidence: 81%

“…Its expression level correlates with tumor predisposition to invasion and metastasis. Our previous study noted a statistically significant decrease in this miRNA expression in grade III-IV gliomas compared to paratumoral tissue [18]. MiRNA -31 inhibits cell migration in glioma cell culture and indirectly regulates activation of the NF-kB transcription factor, angiogenesis, and E-cadherin level through the epithelial-to-mesenchymal transition [24].…”

Section: Discussionmentioning

confidence: 91%

“…It is oncogenic, and its level is increased in the tumor tissues, including the tissues of gliomas [17]. Various data sources show that the miRNA expression level is increased 5-15 times compared to the normal tissue [16][17][18]. The oncogenic effect of miRNA-21 in human glioma cells is implemented through the suppression of such tumor suppressor genes as TAp63, HNRPK, JMY, TOPORS, RECK, TP53BP2, TIMP3, PDCD4, and TGFBR2/3 [19].…”

Section: Discussionmentioning

confidence: 99%

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MicroRNAs in the Diagnosis of Malignancy of Supratentorial Brain Gliomas and Prognosis of Disease Progression

Stupak¹,

Veryasikina²,

Titov³

et al. 2023

Cureus

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Introduction: The study of brain tumors has shown that microRNAs can act as both oncogenes and tumor suppressors and, consequently, can be used as biomarkers for the diagnosis and prognosis of such tumors. Thus, big interest arises in the role of microRNA and its part in oncogenesis in the human brain to find key molecules that can act as tumor markers for diagnostic and prognostic purposes, as well as potential therapeutic agents.Study aim: The sim of this study was to assess histological, molecular, and genetic metrics in patients with supratentorial gliomas, and indicate diagnostic and prognostic abilities of microRNA usage as biomarkers of the grade of malignancy of the tumor.Materials and methods: Clinical and genetic studies were performed in 107 operated patients with supratentorial gliomas of different malignancies. The expression levels of 10 microRNAs (-16, -21¸ -31, -124, -125b, -181b, -191, -221, -223, and -451) were analyzed using real-time polymerase chain reaction (PCR). The results were analyzed statistically using Statistica 12.0 (Statistica, Hamburg, Germany) and GraphPad Prism 9 software (GraphPad Software Inc., Boston, Massachusetts, United States).Results: Based on a comprehensive statistical analysis involving the database of the clinical results of treatment of all 107 patients (combined treatment methods, quality of life, and survival) and microRNA expression levels, specific profiles of microRNA expression typical of different histotypes of gliomas of different malignancy were identified, the prognostic significance of the studied microRNAs as potential predictors of survival in patients with brain gliomas was determined, and microRNAs with the highest prognostic value were identified among them.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 73%