Utilization of arbuscular mycorrhizal spores carrier media which has heavyweight, not optimal utilization of organic material as arbuscular mycorrhiza inoculant carrier, and founded of arbuscular mycorrhizal spores which have wide adaptability and tolerance on land contaminated with heavy metals are the basis for implementation of this research. The aim of this research was to determine combination of organic culture media which is good for increasing the abundance and diameter of indigenous Gigaspora sp. This research was carried out at Universitas Muhammadiyah Parepare, and Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Makassar. The research was compiled using a completely randomized design. The combination treatment of organic culture media used was a combination of rice husk charcoal, sand, zeolite; rice husk charcoal, sand, sawdust; rice husk charcoal, sand, cocopeat; rice husk charcoal, sand, paddy soil; rice husk charcoal, sand, cold larva. The results showed that combination of rice husk charcoal, sand, cocopeat; and combination of rice husk charcoal, sand, paddy soil gives the best results on growth and development of Gigaspora spores. Combination of rice husk charcoal, sand, and cocopeat, can be recommended as media an effective, efficient, and inexpensive spore carrier medium, but should be used after decomposing into compost.