Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza

Wu, Tian; Natran, Annelore; Prost, Lucas; Aydogdu, Eylem; Peer, Yves Van de; Bafort, Quinten

doi:10.1007/978-1-0716-2561-3_19

Cited by 4 publications

(3 citation statements)

References 72 publications

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“…Each of these strains belongs to a different population genetic cluster as distinguished by Xu et al (2019) (see Supplementary Table S1 ). After flow cytometric verification ( Wu et al, 2022 ) of the diploidy of these strains, we induced WGD using a colchicine treatment ( Supplementary methods 1: Colchicine treatment ). Tetraploid lines were selected, and their ploidy was monitored closely for a minimum of 2 months to ensure the stability of the lines.…”

Section: Methodsmentioning

confidence: 99%

The immediate effects of polyploidization ofSpirodela polyrhizachange in a strain-specific way along environmental gradients

Bafort

Natran

et al. 2023

Evolution Letters

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The immediate effects of plant polyploidization are well characterized and it is generally accepted that these morphological, physiological, developmental, and phenological changes contribute to polyploid establishment. Studies on the environmental dependence of the immediate effects of whole-genome duplication (WGD) are, however, scarce but suggest that these immediate effects are altered by stressful conditions. As polyploid establishment seems to be associated with environmental disturbance, the relationship between ploidy-induced phenotypical changes and environmental conditions is highly relevant. Here, we use a common garden experiment on the greater duckweed Spirodela polyrhiza to test whether the immediate effects of WGD can facilitate the establishment of tetraploid duckweed along gradients of two environmental stressors. Because successful polyploid establishment often depends on recurrent polyploidization events, we include four genetically diverse strains and assess whether these immediate effects are strain-specific. We find evidence that WGD can indeed confer a fitness advantage under stressful conditions and that the environment affects ploidy-induced changes in fitness and trait reaction norms in a strain-specific way.

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Section: Methodsmentioning

confidence: 99%

The immediate effects of polyploidization ofSpirodela polyrhizachange in a strain-specific way along environmental gradients

Bafort

Natran

et al. 2023

Evolution Letters

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“…Evolution experiments with real biological organisms might be another means to get further insights into the mechanistic underpinnings explaining why duplicated GRNs might confer a selective advantage for polyploids during stressful times. By “replaying the duplication tape of life”, fitness of nonpolyploid and polyploid species can be evaluated under normal and stressful conditions ( 24 , 73 – 76 ). Transcriptomes can be sequenced, and phenotypic and physiological responses measured and linked to the duplication of genomes and GRNs.…”

Section: Discussionmentioning

confidence: 99%

The duplication of genomes and genetic networks and its potential for evolutionary adaptation and survival during environmental turmoil

Ebadi,

Bafort,

Mizrachi

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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The importance of whole-genome duplication (WGD) for evolution is controversial. Whereas some view WGD mainly as detrimental and an evolutionary dead end, there is growing evidence that polyploidization can help overcome environmental change, stressful conditions, or periods of extinction. However, despite much research, the mechanistic underpinnings of why and how polyploids might be able to outcompete or outlive nonpolyploids at times of environmental upheaval remain elusive, especially for autopolyploids, in which heterosis effects are limited. On the longer term, WGD might increase both mutational and environmental robustness due to redundancy and increased genetic variation, but on the short—or even immediate—term, selective advantages of WGDs are harder to explain. Here, by duplicating artificially generated Gene Regulatory Networks (GRNs), we show that duplicated GRNs—and thus duplicated genomes—show higher signal output variation than nonduplicated GRNs. This increased variation leads to niche expansion and can provide polyploid populations with substantial advantages to survive environmental turmoil. In contrast, under stable environments, GRNs might be maladaptive to changes, a phenomenon that is exacerbated in duplicated GRNs. We believe that these results provide insights into how genome duplication and (auto)polyploidy might help organisms to adapt quickly to novel conditions and to survive ecological uproar or even cataclysmic events.

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“…But is an atlas approach based on the transcriptomic cell type definition the best way forward, or should lineage and tree-based alternatives be considered? Model plants tractable for the whole-organism molecular analyses at single-cell level recommended by Domcke and Shendure (2023) include the tiny duckweed (Spirodela; Wu et al, 2023) and, beyond flowering plants, the liverwort Marchantia (Bowman et al, 2022). Plants have some advantages over animals for lineage tracing, notably that with few exceptions plant cells are immobile.…”

Section: Discussionmentioning

confidence: 99%

The evolving definition of plant cell type

Amini,

Doyle,

Libault

2023

Front. Plant Sci.

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Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza

Cited by 4 publications

References 72 publications

The immediate effects of polyploidization ofSpirodela polyrhizachange in a strain-specific way along environmental gradients

The immediate effects of polyploidization ofSpirodela polyrhizachange in a strain-specific way along environmental gradients

The duplication of genomes and genetic networks and its potential for evolutionary adaptation and survival during environmental turmoil

The evolving definition of plant cell type

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