Stylet penetration behavior of <i>Myzus persicae</i> related to transmission of Zucchini yellow mosaic virus

Symmes, E.; Walker, G. P.; Perring, Thomas M.

doi:10.1111/j.1570-7458.2008.00784.x

Cited by 15 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One biotype of soybean aphid ( Glycine max ) was also shown to have a higher number of potential drops when feeding on resistant genotypes than on susceptible genotypes [ 11 ]. It has been reported that potential drops are related with aphid transmission of non-persistently transmitted viruses [ 68 , 69 ]. However, it is unknown if they are indicative for a specific plant resistance component.…”

Section: Discussionmentioning

confidence: 99%

Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession

et al. 2018

View full text Add to dashboard Cite

BackgroundThe green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations in the field and in the greenhouse. Until now, no strong sources of resistance against the GPA have been identified. Therefore the main aims of this study were to identify pepper materials with a good level of resistance to GPA and to elucidate possible resistance mechanisms.ResultsWe screened 74 pepper accessions from different geographical areas for resistance to M. persicae. After four rounds of evaluation we identified one Capsicum baccatum accession (PB2013071) as highly resistant to M. persicae, while the accessions PB2013062 and PB2012022 showed intermediate resistance. The resistance of PB2013071 resulted in a severely reduced uptake of phloem compared to the susceptible accession, as determined by Electrical Penetration Graph (EPG) studies. Feeding of M. persicae induced the expression of callose synthase genes and resulted in callose deposition in the sieve elements in resistant, but not in susceptible plants.ConclusionsThree aphid resistant pepper accessions were identified, which will be important for breeding aphid resistant pepper varieties in the future. The most resistant accession PB2013071 showed phloem-based resistance against aphid infestation.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1340-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This technique electrically records the changes in resistance that relate to the different stylet penetration activities of sucking insects, providing information on stylet tip position in specific plant tissues and time spent at each location (Tjallingii, 1985). The EPG technique has been applied to understand pathogen transmission by insect vectors (Powell, 1991;Fereres & Collar, 2001;Symmes et al, 2008;Sandanayaka et al, 2013a) by correlating stylet penetration activities to acquisition and inoculation of pathogens by insect vectors (Martin et al, 1997;Tjallingii & Prado, 2001;Stafford et al, 2009;Moreno et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Stylet penetration activities linked to the acquisition and inoculation of Candidatus Liberibacter solanacearum by its vector tomato potato psyllid

Sandanayaka

Moreno²,

Tooman

et al. 2014

Entomologia Exp Applicata

View full text Add to dashboard Cite

The tomato potato psyllid (TPP), Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is the main vector of the bacterium Candidatus Liberibacter solanacearum (Lso), a major disease of solanaceous crops. Feeding of TPP is associated with Lso transmission. However, very little is known about the stylet penetration activities linked to acquisition and inoculation of Lso. The electrical penetration graph (EPG)‐DC system was used to monitor stylet penetration activities during acquisition and inoculation of Lso by individual TPP on tomato [Solanum lycopersicum L. (Solanaceae)]. Female TPP from Lso‐free and Lso‐infected colonies were used in acquisition and inoculation tests, respectively. In the acquisition tests, TPP were tested for Lso after EPG recording of their stylet penetration activities on Lso‐infected tomato shoots. In the inoculation tests, samples from the tomato plants on which the stylet penetration of Lso‐infected TPP had been recorded were tested for Lso infection. The relationships between qPCR results and the EPG waveforms (C, G, D, E1, and E2) representing the main stylet penetration activities performed by individual insects in inoculation and acquisition tests were investigated. Results confirmed that a single adult TPP is capable of infecting a plant with Lso. Our data suggest that acquisition of the bacteria occurs during phloem ingestion (E2), and inoculation is likely associated with salivation into the phloem sieve elements (E1). The durations of EPG parameters were not significantly different between Lso‐infected and Lso‐free TPP (later shown by qPCR) in acquisition tests. In inoculation tests, the durations of E1 or E2 recorded from TPP on Lso‐infected and Lso‐free plants that were later shown by qPCR were not significantly different. However, C was shorter on Lso‐infected plants than on Lso‐free plants, where TPP performed phloem activities. The minimum plant access period required for Lso transmission by a single TPP was estimated to be ca. 2 h, with an acquisition threshold of about 36 min.

show abstract

“…The EPG technique has been widely used to study nonpersistent virus transmission by aphids (e.g., Mart ın et al, 1997;Symmes et al, 2008) as well as resistance, e.g., of Hordeum bulbosum (L.) to Rhopalosiphum padi (L.) (Schliephake et al, 2013), potatoes to M. persicae (Alvarez et al, 2006), and various Brassica forms to Brevicoryne brassicae (L.) (Cole, 1994).…”

Section: Introductionmentioning

confidence: 99%

Feeding behavior of Myzus persicae on asparagus species susceptible and resistant to Asparagus virus 1

Lantos

Schliephake

Krämer

et al. 2019

Entomologia Exp Applicata

View full text Add to dashboard Cite

Asparagus virus 1 (AV‐1) infects Asparagus officinalis L. (Asparagaceae) in the field worldwide. However, various wild relatives of A. officinalis are resistant to AV‐1. Here we study the behavior of the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), on 19 AV‐1‐resistant wild relatives of A. officinalis. We focus on behavior that is associated with regular cell penetration, relevant for inoculation of AV‐1, and sieve element penetration to check for vector resistance and its potential influence on AV‐1 transmission. Parameters, relevant for the transmission of non‐persistent viruses and host plant acceptance, were obtained by the electrical penetration graph technique. Furthermore, phylloclade architecture of A. officinalis and its wild relatives was examined to study its influence on aphid behavior. Behavior of M. persicae displays many cell penetrations and long ingestion periods on A. officinalis, compared to the generally shorter cell penetrations (reduced potential for virus transmission) and reduced or no ingestion (phloem‐located aphid resistance) on wild relatives. Because effects on aphid behavior are not consistent throughout the group of the tested wild relatives of A. officinalis, with some wild relatives being susceptible to M. persicae, a common genetic background for AV‐1 and aphid resistance appears to be unlikely. However, the reduced potential of virus transmission as well as aphid resistance shown by some wild relatives may be useful for future breeding programs.

show abstract

Stylet penetration behavior of Myzus persicae related to transmission of Zucchini yellow mosaic virus

Cited by 15 publications

References 27 publications

Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession

Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession

Stylet penetration activities linked to the acquisition and inoculation of Candidatus Liberibacter solanacearum by its vector tomato potato psyllid

Feeding behavior of Myzus persicae on asparagus species susceptible and resistant to Asparagus virus 1

Contact Info

Product

Resources

About