Styphnolobium japonicum (L.) Schott Fruits Increase Stress Resistance and Exert Antioxidant Properties in Caenorhabditis elegans and Mouse Models

Abstract

Styphnolobium japonicum (L.) Schott is a popular Asian tree widely used in traditional medicine. The current study explored the potential stress resistance and antioxidant activities of its fruits. Phytochemical profiling of the hydroalcoholic fruit extract was done via high performance liquid chromatography-photodiode array-electrospray ionization-mass/mass (HPLC-PDA-ESI-MS/MS). Twenty four phenolic constituents were tentatively identified in the extract. The Caenorhabditis elegans (C. elegans) nematode model in addition to trimethyltin (TMT)-induced neurotoxicity mouse model were used for in vivo evaluation of its antioxidant properties. The ability of the extract to enhance stress resistance was manifested through increasing survival rate by 44.7% and decreasing basal reactive oxygen species (ROS) levels by 72.3% in C. elegans. In addition, the extract increased the levels of the stress response enzyme superoxide dismutase-3 (Sod-3) by 55.5% and decreased the expression of heat shock protein-16.2 (Hsp-16.2) in nematodes, which had been challenged by juglone, by 21%. Using a mouse model, the extract significantly decreased the expression of the oxidative stress marker malondialdehyde (MDA). Furthermore, an elevation in the levels of the antioxidant marker glutathione (GSH), SOD and heme oxygenase-1 (HO-1) enzymes were observed. Our findings imply that Styphnolobium japonicum has the potential to be used in future studies focusing on diseases associated with oxidative stress.

Ishige okamurae Suppresses Trimethyltin-Induced Neurodegeneration and Glutamate-Mediated Excitotoxicity by Regulating MAPKs/Nrf2/HO-1 Antioxidant Pathways

“…Recently, HO-1 in neurodegeneration has received much more attention because it can catalyze heme to bile pigments (biliverdin and bilirubin), which have strong antioxidant properties [ 22 , 23 ]. Thabit S et al reported that the administration of an extract from Styphnolobium japonicum (SJ) fruits to TMT-injected mouse attenuated oxidative stress by recovering the expression of HO-1 in mice brains [ 24 ]. In addition, it was reported that cerebellar granule neurons (CGSs) overexpressing HO-1 showed resistance against glutamate-induced oxidative stress [ 25 ], indicating that HO-1 expression can be a critical defense mechanism against oxidative stress-mediated neurodegeneration.…”

Evaluation of the geroprotective effects of withaferin A in Drosophila melanogaster

“…Blueberry extract [ 57 ], Lonicera japonica extract [ 58 ] promote stress tolerance in Caenorhabditis elegans. Styphnolobium japonicum fruits [ 59 ], Rhodiola rosea root extract [ 60 ], Cotinus coggygria extract [ 61 ] increase stress resistance and exert antioxidant properties in mouse models. Therefore, we decided to check the effect of WA on the resistance of Drosophila’s flies to oxidative stress and hyperthermia.…”

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

“…Peak 20 was shown a pseudomolecular ion at m/z 813. Its major ion at m/z 637 [M -H − 176] − , 595, and 301, gave evidence of glucuronide (176 Da) residue loss and the mass fragment ion pattern of peak 20 was similar to that of peak 11 [33]. Therefore, peak 11 was tentatively identified as quercetin acetylhexoside-pentoside glucuronide.…”