Sub-2 μm fully porous and partially porous (core–shell) stationary phases for reversed phase liquid chromatography

Borges, Endler Marcel; Rostagno, Maurı́cio A.; Meireles, M. Ângela A.

doi:10.1039/c3ra45418e

Cited by 22 publications

(11 citation statements)

References 85 publications

(127 reference statements)

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“…The most current technologically advanced packing materials employ superficially porous particles (SPPs), typically having ~2.7 µm diameters, to produce column efficiencies as great as sub‐2 µm FPPs while allowing for the use of nonspecialized HPLCs and column hardware . SPP‐based achiral columns generally allow for the high efficiency obtained on a sub‐2 µm FPP while avoiding some of the challenges associated with using such small particles.…”

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confidence: 99%

Superficially Porous Particle Based Hydroxypropyl‐β‐cyclodextrin Stationary Phase for High‐Efficiency Enantiomeric Separations

et al. 2015

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A superficially porous particle (SPP)-based hydroxypropyl-β-cyclodextrin (HPBCD) chiral stationary phase (CSP) was produced and its chromatographic performance was compared to both 5 µm and 3 µm fully porous particle (FPP)-based CSPs. The relative surface coverage of the HPBCD chiral selector on each particle was approximately equal, which resulted in equivalent enantiomeric selectivity (α) values on each phase when constant mobile phase conditions were used. Under such conditions, the SPP column resulted in greatly reduced analysis times and three times greater efficiencies compared to the FPP columns. When higher flow rates were used, efficiency gains per analysis times were five times greater for the SPP column compared to the FPP-based columns. When the mobile phases were altered to give similar analysis times on each column, resolution values were doubled for the SPP column. Finally, the novel SPP based HPBCD column proved to be stable for 500 injections under high flow rate (4.5 mL/min) and high pressure (400 bar) conditions used for an ultrafast (~45 sec) enantiomeric separation.

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confidence: 99%

Superficially Porous Particle Based Hydroxypropyl‐β‐cyclodextrin Stationary Phase for High‐Efficiency Enantiomeric Separations

et al. 2015

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“…A silica solid non-porous core coated with a thick porous layer constitutes the phases commercially known as fused-core, solid-core, porous-shell, and core-shell particles. Compared to sub-2 µm fully porous particles, partially porous particles have equivalent performance, but the advantage of lower backpressures, so that they can be even used with conventional HPLC systems [13].…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Strategies for Separation of Bioactive Compounds in Food Matrices

et al. 2018

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Nowadays, there is an increasing attention for nutraceuticals and, in general, bioactive compounds naturally present in food. Indeed, the possibility of preserving human health and preventing disease (e.g., cardiovascular diseases, cancer etc.) by the intake of healthy food is attractive for both consumers and food industries. In turn, research in this field was also prompted significantly, with the aim of characterizing these bioactive compounds and ascribe to them a specific activity. The bioactive compounds can belong to several chemical classes. However, their chemical diversity and presence in complex matrices, such as food, make it challenging both their isolation and characterization. To tackle this issue, efficient separation systems are needed, which are mainly based on chromatography. In this context, this mini-review aims to provide the reader with an overview of the most relevant and recent approaches for the separation of the most common bioactive compounds in food, in particular polyphenols, phenols, carotenoids, and peptides, by liquid chromatography approaches.

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“…However, most applications of sub-2 mm FPP-and SPP-packed columns lie in the analysis of organic compounds by reversed phase liquid chromatography (RPLC). [7][8][9][10] Although hydrophilic interaction liquid chromatography is devoted to separating polar, hydrophilic and/or charged compounds and is well suited for separating metallic complexes, including non-covalent species, 11 application of this separation mode for speciation analysis is still relatively limited. Studies on the speciation of lanthanides have focused so far on gadolinium (Gd) species containing linear or cyclic polyaminocarboxylic acids, used as magnetic resonance imaging contrast agents.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of superficially and fully porous particles for HILIC separation of lanthanide–polyaminocarboxylic species and simultaneous coupling to ESIMS and ICPMS

et al. 2018

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Sub-2 μm fully porous and partially porous (core–shell) stationary phases for reversed phase liquid chromatography

Abstract: The need for increased throughput and superior performance has increased the demand for stationary phases with improved kinetic performance.

Cited by 22 publications

References 85 publications

Superficially Porous Particle Based Hydroxypropyl‐β‐cyclodextrin Stationary Phase for High‐Efficiency Enantiomeric Separations

Superficially Porous Particle Based Hydroxypropyl‐β‐cyclodextrin Stationary Phase for High‐Efficiency Enantiomeric Separations

Liquid Chromatographic Strategies for Separation of Bioactive Compounds in Food Matrices

Evaluation of superficially and fully porous particles for HILIC separation of lanthanide–polyaminocarboxylic species and simultaneous coupling to ESIMS and ICPMS

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