Sub-2 μm Silica Particles in Chiral Separation

Ibrahim, Diana; Ghanem, Ashraf

doi:10.5772/intechopen.79063

Cited by 1 publication

(3 citation statements)

References 49 publications

(175 reference statements)

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“…The Van Deemter equation is frequently used to describe the three contributions to HETP (Dores‐Sousa et al, 2019; Ibrahim & Ghanem, 2018; Łącki, 2018; Vanderlinden et al, 2019):

HETP = A + \frac{B}{u_{f}} + (C_{m} + C_{s}) u_{f}

where

A

B

C_{m}

and

C_{s}

are related to eddy dispersion, molecular diffusion, and the mass transfer resistance in the mobile phase and solid phases, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…When the velocity is high though, the B‐term can be disregarded (Dores‐Sousa et al, 2019). The C‐term is often split into one factor for the mobile phase and one factor for the stationary phase (Ibrahim & Ghanem, 2018). Because the TPMS structure comprises two stationary phases on either side of a mobile‐phase channel in the center, the mass transfer of a tracer in a single flow route in a TPMS monolith is more comparable to that in an open tubular column than it is to packed‐bed chromatography.…”

Section: Resultsmentioning

confidence: 99%

“…Three pieces of functionalized gel (1 mm wide and 2 mm long) were added to a microtube containing the stained yeast cells and shaken overnight. The following day, the three pieces were imaged with a TCS SP5 confocal microscope (Leica Microsystems; Danaher).3 | RESULTS AND DISCUSSION3.1 | HETP of different structuresThe Van Deemter equation is frequently used to describe the three contributions to HETP(Dores-Sousa et al, 2019;Ibrahim & Ghanem, 2018;Łącki, 2018;Vanderlinden et al, 2019): B, C m and C s are related to eddy dispersion, molecular diffusion, and the mass transfer resistance in the mobile phase and solid phases, respectively.Within any single TPMS monolith, there is no need to consider variation in the A-term because there is an absence of nonhomogenous particles and variation in packing quality. The B-term, or longitudinal diffusion, increased with hydraulic diameter.…”

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confidence: 99%

See 2 more Smart Citations

Performance of various 3D‐printed monolith geometries as an alternative to expanded bed adsorption for protein purification

Pei

Feast²,

Holland

et al. 2023

Biotech & Bioengineering

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Here, we compare the performance of various three‐dimensional‐printed Monolith Adsorption (PMA) columns designed from a triply periodic minimal surface geometry, the Schoen gyroid. The structures examined had designed hydraulic diameters between 203 and 458 µm and voidages of 40%−60%. We compare column efficiency, porosity, static binding capacity and dynamic binding capacity for various load volumes and flow rates. The results show that all structures allowed efficient passage of yeast cells (>97%) over a wide range of interstitial velocities (191 to 1911 cm/h) while maintaining a low pressure drop (<0.1 MPa). The structure with a voidage of 40% and a hydraulic diameter of 203 µm showed the best performance in all aspects evaluated. Bovine serum albumin (BSA) recoveries for all structures (27%−91% when the loaded volume was 180 mL) were significantly affected by hydraulic diameter, mean channel wall thickness, velocity and voidage. Moreover, biomass addition resulted in a decrease in BSA recovery, which became more obvious at high velocities. However, this did not lead to a dramatic reduction in saturated binding capacity, significant changes in axial dispersion, or blockage of channels and could be compensated for by recirculation of the feed, even at high velocity. PMA thus potentially provides an appealing alternative to Expanded Bed Adsorption, retaining the latter's advantages, while eliminating fluidization issues and minimizing both processing time and buffer consumption.

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“…The Van Deemter equation is frequently used to describe the three contributions to HETP (Dores‐Sousa et al, 2019; Ibrahim & Ghanem, 2018; Łącki, 2018; Vanderlinden et al, 2019):