Sub-acute toxicity of Ashwagandha (Withania somnifera) root extract in wistar rats

Langade, Deepak; Dawane, Jayshree; Dhande, Priti

doi:10.1016/j.toxrep.2023.10.009

Cited by 7 publications

(1 citation statement)

References 20 publications

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“…Win is a characteristic of withanolide with toxcophores that could cause antagonistic drug reactions when taken in excess of the prescribed dose limit. 28 Baghel et al, (2023) observed the reduced activity of Superoxide Dismutase (SOD) in the pancreas, brain, and testes of diabetic mice (Streptozotocin STZ). The catalase activity was increased following administration of Withaferin-A (WA) to rats and the values of WA+STZ group was higher than the STZ group.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Sivanandam,

Senthilkumar,

Dipti

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Nandrolone decanoate (ND) is a commonly used androgen-based steroid that causes adverse effects on illicit self-administered users to boost their physical endurance. Although, it has an anabolic effect on the skeletal muscles, plausible evidence supports that the gonadal toxicity of ND can cause damage to reproductive functions. Aim: The present study investigated the possible protective effects of Withania somnifera (WS) root extract (200 and 400 mg/kg bw) on ND-induced (10 mg/kg bw) male gonadal toxicity in rats. Materials and Methods:The present seminal research was not experimented in any of the earlier studies. ND was induced in rats by intramuscular injection bi-weekly for 30 days. The aqueous extract of WS root powder was delivered orally once daily for 30 days to the ND-treated group. Hematoxylin-and-eosin-marked sections of the testes were studied. Results: The testosterone levels significantly decreased while there was an increase in the malondialdehyde levels among the ND-induced group against the control group. The ND induced rats treated with varying doses of WS showed a significant reversal of the gonadal toxicity by improvement in the testosterone and antioxidant parameters. The sperm count and sperm motility rate were increased significantly after WS therapy. Furthermore, the testis morphology of the WS-treated group indicated preserved germinal cells, active spermatogenesis, and absence of atrophy, vacuolation, and necrosis. Conclusion: WS treatment in ND-induced male rats alleviated the toxic effects of the ND on the gonads, probably due to its anti-inflammatory and antioxidant properties.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Sivanandam,

Senthilkumar,

Dipti

et al. 2024

Ind. J. Pharm. Edu. Res

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Natural antioxidants: A comprehensive elucidation of their sources, mechanisms, and applications in health

Chibuye,

Singh,

Ramasamy

et al. 2024

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Various investigations of ameliorative role of Ashwagandha seeds (Withania somnifera) against amoxicillin toxicity

Elnasharty,

Elwan,

Elhadidy

et al. 2024

Toxicology Research

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Several studies showed the adverse effects of amoxicillin on various body organs. So, this research has been designed to evaluate the modulatory role of Ashwagandha seed extract (ASE) against amoxicillin (AM) toxicity. Rats treated with AM (90 mg/kg), protected by ASE doses (100, 200 and 300 mg/kg), and treated by ASE at the same three doses. At the end of the experimental period, DNA comet assay, cytogenetic examinations, sperm-shape analysis, evaluation of the malondialdehyde (MDA) percentages, histopathological examinations, and biophysical tests (modulus, relaxation time, permittivity, entropy, and internal energy change of brain) were documented. The results confirmed that AM treatment induced significant elevation of DNA damage, cytogenetic aberrations, and MDA content in brain, liver, and testis tissues and sperm-shape anomalies. ASE treatment significantly minimized the genetic changes, sperm-shape anomalies, and MDA generation. These enhancements were more pronounced by protective ASE and increased by increasing the dose level. In histopathological examinations, AM treatment caused neurotoxicity in brain tissue. ASE treatment, partially, minimized these damages and the positive effects of therapeutic ASE were more noticeable. Biophysical parameters showed that therapeutic ASE was better for relaxation time, permittivity, and free energy change. Protective and therapeutic ASE were able to recover entropy and internal energy changes in variant degrees.

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Sub-acute toxicity of Ashwagandha (Withania somnifera) root extract in wistar rats

Cited by 7 publications

References 20 publications

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Natural antioxidants: A comprehensive elucidation of their sources, mechanisms, and applications in health

Various investigations of ameliorative role of Ashwagandha seeds (Withania somnifera) against amoxicillin toxicity

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