Sub-arm location of prolamin and EST-SSR loci on chromosome 1Hch from Hordeum chilense

Abstract

Hordeum chilense Roem. et Schult. is a diploid wild South American barley that contains genes of interest for cereal breeding, many of them located on chromosome 1H ch . In the current study, two H. chilense-wheat addition lines with deletions in the 1H ch chromosome were used for sub-arm localization of five prolamin (glutenin and gliadin) loci and 33 EST-SSR marker loci on chromosome 1H ch . The two sets of markers were distributed across five sub-arm chromosome regions. Three glutenin loci (Glu-H ch 2, Glu-H ch 3, Glu-H ch 4) together with the gliadin locus Gli-H ch 1 were located on the distal 20% of the 1H ch S arm, whereas the glutenin locus Glu-H ch 1 was on the proximal 88% region of 1H ch L. Among 33 EST-SSR marker loci, 7 (21.2%) were on the 1H ch S arm and, of them, 3 (9.1%) were on the distal 20% end and 4 (12.1%) on the proximal 80% region. The 26 loci (78.8%) on 1H ch L were distributed across three different regions: 18 (78.8%) in the proximal 88%, 3 (9.1%) in the distal 12% and 5 (15.2%) in a region less than 12% from the distal end. The deletions in the 1H ch chromosome added to the common wheat background were thus shown to be useful for determining the sub-arm location of EST-SSR and prolamin loci. This could facilitate the identification of molecular markers linked to genes of agronomic interest and the isolation of such genes for use in common wheat improvement.

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

“…The availability of molecular markers capable of distinguishing between H. chilense chromatin in wheat background constitutes an important tool for selection. Barley expressed sequence tag (EST) have been successfully transferred to H. chilense (Hagras et al 2005a, b) and they have been used for physical mapping of chromosomes 4H ch (Said and Cabrera 2009), 1H ch (Cherif-Mouaki et al 2011) and 3H ch (Said et al 2012). In addition, the development of the conserved orthologous set (COS) (Quraishi et al 2009) signifies an additional source of markers potentially useful for H. chilense.…”

“…Indeed, from a set of 1,165 barley markers, a total of 510 (43.8 %) successfully amplified in H. chilense(Hagras et al 2005a). Nevertheless, these markers are very useful for the identification of H. chilense chromatin into wheat background(Atienza et al 2007c;Castillo et al 2014;Said and Cabrera 2009;Cherif-Mouaki et al 2011;Said et al 2012). Previous studies have yielded a limited set of markers useful for H. chilense including RAPDs, SCARs, SSRs and STS (reviewed byHernandez 2005) with barley and wheat SSRs providing a transferability rate of 53.5 %(Hernandez et al 2002).…”

Isolation and Characterization of a Psathyrostachys huashanica Keng 6Ns Chromosome Addition in Common Wheat

“…Indeed, the production of alien addition lines is an important step during successful gene transfer to wheat because it plays a bridging role in the creation of alien substitution lines and translocation lines. Useful traits have been introduced successfully from wild relatives, including Thinopyrum elongatum [5], Secale cereale L. [6], Leymus racemosus [7], and Hordeum chilense [8], into hexaploid wheat via the development of alien addition lines.…”

Hordeum chilense genome, a useful tool to investigate the endosperm yellow pigment content in the Triticeae

“…Second, the diploid constitution of this species and the existence of H . chilense -wheat chromosome addition lines [44] and other deletion stocks [45,46] makes the identification of candidate genes more accessible. Third, a high density genetic map has been recently developed using H .…”