Sub-cellular partitioning of cadmium, copper, nickel and zinc in indigenous yellow perch (Perca flavescens) sampled along a polymetallic gradient☆

Giguère, Anik; Campbell, Peter G. C.; Hare, Landis; Couture, Patrice

doi:10.1016/j.aquatox.2005.12.001

Cited by 125 publications

(113 citation statements)

References 35 publications

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“…The increase of detoxified fractions (HSP and MRG) in the old leaves with increased Cd dose (Fig. 1b) presumably reflects the progressive Cd detoxification by metallothionein-like proteins produced in cultivar Zibo-3 (Giguere et al, 2006). In addition, Cd associated with the HDP and organelle fractions increased in the 5 mg/L Cd treated samples compared to the 2 mg/L Cd treated samples, which could explain the increased Cd toxicity in the Zibo-9 samples.…”

Section: Subcellular Distributionmentioning

confidence: 74%

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Zhang

Guo

Yang

et al. 2015

Ecotoxicology and Environmental Safety

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a b s t r a c tThe aims of this study were: (1) the study of cadmium (Cd) accumulation and toxicity in different castor cultivars (Ricinus communis L.); (2) to investigate changes in antioxidant enzymatic activities and the subcellular distribution of Cd in young and old leaves from two different castor cultivars, after exposure to two different Cd concentrations, and explore the underlying mechanism of Cd detoxification focusing on antioxidant enzymes and subcellular compartmentalization. The Cd concentration, toxicity, and subcellular distribution, as well as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were measured in Zibo-3 and Zibo-9 cultivars after exposure to two different concentrations of Cd (2 mg/L and 5 mg/L) for 10 days. This research revealed Cd accumulation characteristics in castor are root4 stem4 young leaf 4old leaf. Castor tolerance was Cd dose exposure and the cultivars themselves dependent. Investigation of subcellular Cd partitioning showed that Cd accumulated mainly in the heat stable protein (HSP) and cellular debris fractions, followed by the Cd rich granule (MRG), heat denatured protein (HDP), and organelle fractions. With increasing Cd concentration in nutrient solution, the decreased detoxified fractions (BDM) and the increased Cd-sensitive fractions (MSF) in young leaves may indicate the increased Cd toxicity in castor cultivars. The BDM-Cd fractions or MSF-Cd in old leaves may be linked with Cd tolerance of different cultivars of castor. The antioxidant enzymes that govern Cd detoxification were not found to be active in leaves. Taken together, these results indicate Cd tolerance and toxicity in castor can be explained by subcellular partitioning.Crown

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Section: Subcellular Distributionmentioning

confidence: 74%

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Zhang

Guo

Yang

et al. 2015

Ecotoxicology and Environmental Safety

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“…In contrast, with increasing whole tissue concentration, more Cu was found in organelles. This decreased relative contribution of Cd and Zn in the HDP fraction, combined with an increased proportion of Cd in the HSP fraction was also observed in perch liver (P. flavescens) by Giguère et al (2006).…”

Section: Regression Summarymentioning

confidence: 87%

“…The extent to which organisms are able to protect themselves against metal toxicity seems species and tissue specific (Wang et al, 1999;Campbell et al, 2005;Giguère et al, 2006).…”

Section: Regression Summarymentioning

confidence: 99%

“…in the freshwater fish, Perca flavescens (Giguère et al, 2006), in the freshwater bivalve, Pyganodon grandis (Campbell et al, 2005) and in aquatic insects (Cain et al, 2004). These organisms all showed a gradual increase of metal concentrations in various metal-sensitive and metal-detoxified sub-cellular fractions with increasing environmental metal levels.…”

Section: Regression Summarymentioning

confidence: 99%

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Differences in metal sequestration between zebra mussels from clean and polluted field locations

et al. 2009

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a b s t r a c tOrganisms are able to detoxify accumulated metals by, e.g. binding them to metallothionein (MT) and/or sequestering them in metal-rich granules (MRG). The different factors involved in determining the capacity or efficiency with which metals are detoxified are not yet known.In this work we studied how the sub-cellular distribution pattern of cadmium, copper and zinc in whole tissue of zebra mussels from clean and polluted surface waters is influenced by the total accumulated metal concentration and by its physiological condition. Additionally we measured the metallothionein concentration in the mussel tissue. Metal concentration increased gradually in the metal-sensitive and detoxified sub-cellular fractions with increasing whole tissue concentrations. However, metal concentrations in the sensitive fractions did not increase to the same extent as metal concentrations in whole tissues. In more polluted mussels the contribution of MRG and MT became more important. Nevertheless, metal detoxification was not sufficient to prevent metal binding to heat-sensitive low molecular weight proteins (HDP fraction). Finally we found an indication that metal detoxification was influenced by the condition of the zebra mussels. MT content could be explained for up to 83% by variations in Zn concentration and physiological condition of the mussels.

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“…For example, metals could be detoxified in a form of metal rich granules, which will be separated within the pellets already after centrifugation of tissue homogenate at 10 000×g, together with cellular debris and mitochondria (Campbell et al 2005;Giguère et al 2006). Centrifugation of liver homogenate at 50 000×g, as used in our study for isolation of soluble hepatic fraction (SOP 1999), therefore enables determination of metals and metalloids in potentially metal-sensitive sub-cellular pools (Giguère et al 2006). …”

Section: Introductionmentioning

confidence: 99%

Multielement analysis in the fish hepatic cytosol as a screening tool in the monitoring of natural waters

Dragun

Fiket

Vuković

et al. 2012

Environ Monit Assess

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The possibility of direct measurement of trace elements in hepatic cytosol of European chub (Squalius cephalus) by HR ICP-MS after cytosol dilution with Milli-Q water and subsequent acidification was investigated. Due to low detection limits of this procedure, determination of 13 elements (As, Cd, Co, Cu, Fe, Mn, Mo, Pb, Sb, Sn, Sr, V, Zn) was possible in the chub hepatic cytosol, exhibiting excellent measurement repeatability in duplicates. Some of these elements were also measured by HR ICP-MS in acid digested cytosols (Cd, Co, Cu, Fe, Mn, Mo, Sr, V, Zn). Good agreement of the results obtained after sample dilution and sample digestion indicated that complex organic matrix of hepatic cytosol did not affect measurement reliability. Cytosolic concentrations of 13 trace elements in the chub liver were quantified in the following order:Fe,Zn>Cu>Mn>Mo>Sr,V,Cd>Co>As,Pb>Sn>Sb. Unlike Cd, Cu, Fe, Mn and Zn for which the cytosolic concentrations were previously reported after measurement by AAS, cytosolic concentrations of eight additional trace elements characteristic for the liver of chubs inhabiting the low contaminated river water were reported here for the first time (ng g -1 ): Mo 136. and Sb 0.9-2.6. The simultaneous measurement of large number of trace elements in the cytosolic fractions of fish tissues, which comprise potentially metal-sensitive sub-cellular pools, could be beneficial as a screening tool in the monitoring of natural waters, because it would enable timely recognition of increased fish exposure to metals.

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Sub-cellular partitioning of cadmium, copper, nickel and zinc in indigenous yellow perch (Perca flavescens) sampled along a polymetallic gradient☆

Cited by 125 publications

References 35 publications

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Differences in metal sequestration between zebra mussels from clean and polluted field locations

Multielement analysis in the fish hepatic cytosol as a screening tool in the monitoring of natural waters

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