Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells

Lin, Yong; Irani, Niloufer G.; Grotewold, Erich

doi:10.1186/1471-2229-3-10

Cited by 36 publications

(11 citation statements)

References 32 publications

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“…Then, similarly to other metabolites, the flavonoid allocation could occur through different parallel pathways, the details of which are still poorly understood. Microscopy analyses by Lin and co-workers [73] have shown that phytochemicals are transported by at least two distinct vesicle trafficking pathways, addressed either to cell wall or to vacuole. The first one is a trans Golgi network (TGN)-independent pathway, suggesting that it is different from the secretion pathway of most proteins.…”

Section: Transport Mediated By Vesicle Trafficking In Plant Cellsmentioning

confidence: 99%

Plant Flavonoids—Biosynthesis, Transport and Involvement in Stress Responses

Petrussa

Braidot

Zancani

et al. 2013

IJMS

533

346

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This paper aims at analysing the synthesis of flavonoids, their import and export in plant cell compartments, as well as their involvement in the response to stress, with particular reference to grapevine (Vitis vinifera L.). A multidrug and toxic compound extrusion (MATE) as well as ABC transporters have been demonstrated in the tonoplast of grape berry, where they perform a flavonoid transport. The involvement of a glutathione S-transferase (GST) gene has also been inferred. Recently, a putative flavonoid carrier, similar to mammalian bilitranslocase (BTL), has been identified in both grape berry skin and pulp. In skin the pattern of BTL expression increases from véraison to harvest, while in the pulp its expression reaches the maximum at the early ripening stage. Moreover, the presence of BTL in vascular bundles suggests its participation in long distance transport of flavonoids. In addition, the presence of a vesicular trafficking in plants responsible for flavonoid transport is discussed. Finally, the involvement of flavonoids in the response to stress is described.

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Section: Transport Mediated By Vesicle Trafficking In Plant Cellsmentioning

confidence: 99%

Plant Flavonoids—Biosynthesis, Transport and Involvement in Stress Responses

Petrussa

Braidot

Zancani

et al. 2013

IJMS

533

346

View full text Add to dashboard Cite

show abstract

“…It could be further demonstrated that anthocyanins are transported to the cell wall or the vacuole by at least two distinct vesicle trafficking pathways (Lin et al, 2003). The first leads to the accumulation of anthocyanic vacuolar inclusions – the dark red- to purple-pigmented spherical bodies in vacuole known also as AVIs.…”

Section: Transport Mediated By Vesicle Trafficking In Plant Cellsmentioning

confidence: 99%

Transporters in plant sulfur metabolism

2014

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Sulfur is an essential nutrient, necessary for synthesis of many metabolites. The uptake of sulfate, primary and secondary assimilation, the biosynthesis, storage, and final utilization of sulfur (S) containing compounds requires a lot of movement between organs, cells, and organelles. Efficient transport systems of S-containing compounds across the internal barriers or the plasma membrane and organellar membranes are therefore required. Here, we review a current state of knowledge of the transport of a range of S-containing metabolites within and between the cells as well as of their long distance transport. An improved understanding of mechanisms and regulation of transport will facilitate successful engineering of the respective pathways, to improve the plant yield, biotic interaction and nutritional properties of crops.

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“…There are many low molecular weight substances derived from or related to phenylpropanoid metabolism—structurally so similar to anthocyanins, that it is reasonable to assume that at least some of them might be also transported in similar autophagy-related membrane containers. It was observed already, more than ten years ago, that phytochemicals with distinct autofluorescence accumulate within membranous bodies, which are differentially targeted to the vacuole or apoplast [30]. Some of these bodies resemble the branched tubular structures mentioned above.…”

Section: Endoplasmic Reticulum Initiated Autophagy As a General Pathwmentioning

confidence: 99%

“…In model Lithospermum erythrorhizon cells and hairy root cultures after synthesis in the ER, the membrane containers with shikonin are bound to the PM [56,57], allowing one to speculate also about a possible autophagy-related mechanism of this process. The most vivid example of possible autophagy-related exosome secretion was provided in maize cells; upon the induction of the P1 transcription factor, autofluorescent membrane-bound bodies (possibly ER derived) accumulate in the cytoplasm and fuse with the PM, releasing their autofluorescent content into the cell wall in an exosome-like manner [30]. Phenylpropanoids, such as hydroxycinnamic acid derivatives, are found in the cell walls esterified to the wall polysaccharides; they are synthesized at the ER, and from there, they are released in a small membrane vesicles, which aggregate into bigger structures, fusing with the PM and releasing the content into the apoplast [58].…”

Section: Putative Autophagy-related Export Of Secondary Metabolites Tmentioning

confidence: 99%

Autophagy-Related Direct Membrane Import from ER/Cytoplasm into the Vacuole or Apoplast: A Hidden Gateway also for Secondary Metabolites and Phytohormones?

Žárský

2014

IJMS

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Transportation of low molecular weight cargoes into the plant vacuole represents an essential plant cell function. Several lines of evidence indicate that autophagy-related direct endoplasmic reticulum (ER) to vacuole (and also, apoplast) transport plays here a more general role than expected. This route is regulated by autophagy proteins, including recently discovered involvement of the exocyst subcomplex. Traffic from ER into the vacuole bypassing Golgi apparatus (GA) acts not only in stress-related cytoplasm recycling or detoxification, but also in developmentally-regulated biopolymer and secondary metabolite import into the vacuole (or apoplast), exemplified by storage proteins and anthocyanins. We propose that this pathway is relevant also for some phytohormones’ (e.g., auxin, abscisic acid (ABA) and salicylic acid (SA)) degradation. We hypothesize that SA is not only an autophagy inducer, but also a cargo for autophagy-related ER to vacuole membrane container delivery and catabolism. ER membrane localized enzymes will potentially enhance the area of biosynthetic reactive surfaces, and also, abundant ER localized membrane importers (e.g., ABC transporters) will internalize specific molecular species into the autophagosome biogenesis domain of ER. Such active ER domains may create tubular invaginations of tonoplast into the vacuoles as import intermediates. Packaging of cargos into the ER-derived autophagosome-like containers might be an important mechanism of vacuole and exosome biogenesis and cytoplasm protection against toxic metabolites. A new perspective on metabolic transformations intimately linked to membrane trafficking in plants is emerging.

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Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells

Cited by 36 publications

References 32 publications

Plant Flavonoids—Biosynthesis, Transport and Involvement in Stress Responses

Plant Flavonoids—Biosynthesis, Transport and Involvement in Stress Responses

Transporters in plant sulfur metabolism

Autophagy-Related Direct Membrane Import from ER/Cytoplasm into the Vacuole or Apoplast: A Hidden Gateway also for Secondary Metabolites and Phytohormones?

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