Sub‐epidermal imaging using polarized light spectroscopy for assessment of skin microcirculation

O’Doherty, Jim; Henricson, Joakim; Anderson, Chris; Leahy, Martin J.; Nilsson, Gert; Sjöberg, Folke

doi:10.1111/j.1600-0846.2007.00253.x

Cited by 115 publications

(147 citation statements)

References 33 publications

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“…In contrast to LDF, it measures RBC concentration in the skin (O'Doherty et al, 2007). Initial investigations show that the technique offers improved sensitivity in measuring vasoconstriction in the skin during iontophoresis, although more research is needed to validate the performance of the technique against the traditional laser Doppler-based methods of perfusion measurement (Farnebo et al, 2010;Petersen et al, 2010;Zhai et al, 2009).…”

Section: Vasoconstrictionmentioning

confidence: 99%

Transdermal iontophoresis as an in-vivo technique for studying microvascular physiology

Tesselaar

Sjöberg

2011

Microvascular Research

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Section: Vasoconstrictionmentioning

confidence: 99%

Transdermal iontophoresis as an in-vivo technique for studying microvascular physiology

Tesselaar

Sjöberg

2011

Microvascular Research

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“…near infrared imaging techniques, optical coherence, polarised light spectroscopy) [33][34][35][36], cannot be used for imaging (not even two-dimensionally) unborn mice. In vivo imaging techniques, which permit 2D in utero analysis of mouse embryos or fetus, and in principle could be used for the generation of volume data, are briefly discussed below.…”

Section: In Vivo Volume Data Generation Methodsmentioning

confidence: 99%

Three-Dimensional (3D) Visualisation of the Cardiovascular System of Mouse Embryos and Fetus

Weninger¹,

Geyer²

2009

TOCARIJ

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Abstract:The mouse is the most appropriate biomedical model organism for researching the mechanistic function of genetic factors in normal embryogenesis and in the genesis of cardiovascular malformations. Three-dimensional (3D) visualisation of the developing organs of wild type and genetically modified mouse embryos is essential for such research. This paper aims at discussing imaging methods that permit the generation of digital volume data sets, which fit for the virtual 3D visualisation and 3D analysis of the cardiovascular system of mouse embryos and mouse fetus. To cover the spectrum of imaging methods comprehensively, we will start with a short overview about early 3D-reconstruction techniques. This will be followed by a brief discussion why in vivo imaging techniques, such as ultrasound (US) or optical coherence tomography (OCT) do not fit for constructing volume data sets that permit virtual 3D visualisation of the cardiovascular system of mouse embryos/fetus. We will then briefly introduce techniques, which permit 3D analysis of the cardiovascular system but do not fit for creating digital volume data (corrosion casts, scanning electron microscopy). Finally, we will focus on describing the advantages and disadvantages, the spectrum of application and the limitations of important modern digital volume data generation methods, such as micro-computed tomography ( CT), micro-magnetic resonance imaging ( MRI), optical projection tomography (OPT), confocal microscopy, histological section based volume data generation methods, and 3D episcopic imaging methods.

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“…High-resolution color images were acquired under standardized conditions, fixed distance, and cross-polarization (Nikon D-90 camera, 60 mm Micro-Nikkor lens, Nikon R1 wireless close-up flash, Nikon) to visualize the sub-epidermal microvasculature 21 and separated into L, a*, and b* images (ImageJ, National Institutes of Health, Bethesda, Maryland). 22,23 Excess erythema was calculated from the red (a*) image as percentage of red pixels above the mean plus 1 SD ( ϩ ) threshold.…”

Section: Skin Evaluationmentioning

confidence: 99%

Face Masks for Noninvasive Ventilation: Fit, Excess Skin Hydration, and Pressure Ulcers

et al. 2015

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BACKGROUND: Pressure ulcers (stages III and IV) are serious safety events (ie, never events).Healthcare institutions are no longer reimbursed for costs to care for affected patients. Medical devices are the leading cause of pediatric pressure ulcers. Face masks for noninvasive ventilation were associated with a high percentage of pressure ulcers at our institution. METHODS: A prospective cohort study investigated factors contributing to pressure ulcer development in 50 subjects using face masks for noninvasive ventilation. Color imaging, 3-dimensional surface imaging, and skin hydration measurements were used to identify early skin compromise and evaluate 3 interventions to reduce trauma: (1) a silicone foam dressing, (2) a water/polyethylene oxide hydrogel dressing, and (3) a flexible cloth mask. A novel mask fit technique was used to examine the impact of fit on the potential for skin compromise. RESULTS: Fifty subjects age 10.4 ؎ 9.1 y participated with color images for 22, hydration for 34, and mask fit analysis for 16. Of these, 69% had diagnoses associated with craniofacial anomalies. Stage I pressure ulcers were the most common injury. Skin hydration difference was 317 ؎ 29 for sites with erythema versus 75 ؎ 28 for sites without erythema (P < .05) and smallest for the cloth mask (P < .05). Fit distance metrics differed for the nasal, oronasal, and face shield interfaces, with threshold distances being higher for the oronasal mask than the others (P < .05). Areas of high contact were associated with skin erythema and pressure ulcers. CONCLUSIONS: This fit method is currently being utilized to select best-fit masks from available options, to identify the potential areas of increased tissue pressure, and to prevent skin injuries and their complications. Improvement of mask fit is an important priority for improving respiratory outcomes. Strategies to maintain normal skin hydration are important for protecting tissue integrity.

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Sub‐epidermal imaging using polarized light spectroscopy for assessment of skin microcirculation

Cited by 115 publications

References 33 publications

Transdermal iontophoresis as an in-vivo technique for studying microvascular physiology

Transdermal iontophoresis as an in-vivo technique for studying microvascular physiology

Three-Dimensional (3D) Visualisation of the Cardiovascular System of Mouse Embryos and Fetus

Face Masks for Noninvasive Ventilation: Fit, Excess Skin Hydration, and Pressure Ulcers

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