Sub-lethal combined effects of illicit drug and decreased pH on marine mussels: A short-time exposure to crack cocaine in CO2 enrichment scenarios

Souza, Lorena da Silva; Bonnail, Estefanía; Maranho, Luciane Alves; Pusceddu, Fábio Hermes; Cortez, Fernando Sanzi; César, Augusto; Ribeiro, Daniel Araki; Riba, Inmaculada; Abessa, Denis Moledo de Souza; DelValls, Ángel; Pereira, Camilo Dias Seabra

doi:10.1016/j.marpolbul.2021.112735

Cited by 4 publications

(5 citation statements)

References 57 publications

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“…According to the criteria established by [84], in the cytochemical method, animals are considered to be stressed but compensating if NRRT were between 50 and 120 min. However, studies have demonstrated that lysosomal membrane stability differs in tropical zones compared to temperate zones, as higher temperatures in the tropics lead the neutral red dye to be retained in the lysosomes for a shorter period of time [85], agreeing with the results of this work and previous studies on NRRT of P. perna populations from the Brazilian coast [43,65,86,87]. Therefore, the present study may contribute to the development of a health index for tropical mussels.…”

Section: Biological Responsessupporting

confidence: 91%

“…To simulate the acidification process based on CO 2 enrichment event such as CCS, a CO 2 injection system was adapted from the experimental set up described by [63,64], with further detailed experiments in [65]. Briefly, the different acidification scenarios reached pH values of 8.0, 7.5, 7.0, 6.5, and 6.0, controlled using the injection system, and non-CO 2 injected treatment as control (pH 8.3).…”

Section: Laboratory Testsmentioning

confidence: 99%

“…6.0210.100) (pH scale calibration with 0.1 M HCl). Dissolved inorganic carbon (DIC) was determined via the experimental values and the seawater carbonate system speciation: HCO 3 − , CO 3 2− , CO 2 , calcite saturation (Ω Cal ), aragonite saturation index (Ω Arag ) and partial pressure of carbon dioxide (pCO 2 ), using the CO2SYS v2.1 program as described in [65].…”

Section: Chemical Characterizationmentioning

confidence: 99%

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Integrated Assessment of CO2-Induced Acidification Lethal and Sub-Lethal Effects on Tropical Mussels Perna perna

et al. 2023

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Leakages of CO2 capture and storage systems from the seabed are able to cause significant adverse biological effects in marine species. Adult mussels were exposed to different CO2 enrichment scenarios (pH from 8.3 to 6.0) for 96 h, and endpoints (lysosomal membrane deterioration, lipid peroxidation and primary damages in DNA) were assessed. Mortality and reduced health status can occur after short exposure of the tropical mussel Perna perna to pH levels lower than 7.5. Results pointed out cytogenotoxic effects in the hemolymph and gills after 48 and 96 h of exposure, respectively. These findings should be considered when environmental monitoring approaches are performed in tropical marine areas employing CCS strategies.

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Section: Biological Responsessupporting

confidence: 91%

Section: Laboratory Testsmentioning

confidence: 99%

Section: Chemical Characterizationmentioning

confidence: 99%

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Integrated Assessment of CO2-Induced Acidification Lethal and Sub-Lethal Effects on Tropical Mussels Perna perna

et al. 2023

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“…Many studies have been performed in the last few years in order to analyze the potential effects from CO 2 enrichment in the ocean caused by both increases in CO 2 atmosphere concentrations and natural uptake on seawater surface, and by CO 2 leakages during the CCS process (e.g., [7][8][9][10]), as well as in combination with harmful substances [11,12].…”

Section: Introductionmentioning

confidence: 99%

Integrative Assessment of Sediments Affected by CO2 Enrichment: A Case Study in the Bay of Santos—SP, Brazil

et al. 2021

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CO2 enrichment in the marine environment caused by leakages from carbon capture and storage technologies may occur over operational procedures. An integrated approach using weight-of-evidence was applied to assess the environmental risk associated with the acidification caused by CO2 enrichment in coastal sediments from Santos (Brazil). Chemical analyses (metal(loid)s and organic contaminant (e.g., hydrocarbons), toxicity tests (amphipods mortality, sea-urchin embryo-larval development) and macro-benthic community structure alteration assessment were performed with different acidified scenarios (pH 8.0–6.0) for two stations with different contamination degrees. These lines of evidence were statistically analyzed and integrated (multivariate analysis and ANOVA). Results of toxicity showed significant chronic effects starting at pH 7.0 while acute effects were observed starting at pH 6.5. The macro-benthic community integrity showed significant differences for all treatments at the Piaçaguera channel station, considered to be moderately contaminated. Results from the multivariate analysis correlated toxic effects and increase in the mobility of some elements with acidification. Also, the biological indexes were correlated with concentrations of dissolved Zn in seawater. The pH of 6.0 was extremely toxic for marine life due to its high acidification and metal bioavailability. The approach herein identified and discriminated the origin of the degradation caused by the acidification related to the enrichment of CO2.

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“…Pollutants also frequently alter their toxicity depending on climate conditions, and chemicals often impact the ability of organisms to adapt to environmental fluctuations (Landis et al 2014;Nikinmaa 2013). Low pH is reported to affect mussel biological responses when exposed in combination with pharmaceutical products (Mezzelani et al 2021), heavy metals (Han et al 2014) or illicit drugs (da Silva Souza et al 2021). Likewise, the responses to plasticisers and other contaminants on mussels could be altered by concomitant changes in ocean chemistry with respect to CO 2 -induced ocean acidification, even considering preadaptation to pH-fluctuating current environments.…”

Section: Introductionmentioning

confidence: 99%

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

Mincarelli

Chapman

Rotchell

et al. 2022

Environ Sci Pollut Res

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Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related genes (i.e. sod, cat, hsp70). We found a significant effect of DEHP on stress response-related gene expression that was dependent on the gametogenesis stage, but there was only a trend towards downregulation of CA2 in response to pH 7.7. In addition, differences in gene expression between males and females were most pronounced in experimental conditions containing DEHP and/or acidified pH but never the control, indicating that it is important to consider sex and gametogenesis stage when studying the response of mussels to diverse stressors.

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Sub-lethal combined effects of illicit drug and decreased pH on marine mussels: A short-time exposure to crack cocaine in CO2 enrichment scenarios

Cited by 4 publications

References 57 publications

Integrated Assessment of CO2-Induced Acidification Lethal and Sub-Lethal Effects on Tropical Mussels Perna perna

Integrated Assessment of CO2-Induced Acidification Lethal and Sub-Lethal Effects on Tropical Mussels Perna perna

Integrative Assessment of Sediments Affected by CO2 Enrichment: A Case Study in the Bay of Santos—SP, Brazil

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

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