Sub-lethal effects of the triazole fungicide propiconazole on zebrafish (Danio rerio) development, oxidative respiration, and larval locomotor activity

Souders, Christopher L.; Xavier, Priscilla; Perez‐Rodriguez, Veronica; Ector, Naomi; Zhang, Jiliang; Martyniuk, Christopher J.

doi:10.1016/j.ntt.2019.106809

Cited by 59 publications

(19 citation statements)

References 46 publications

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“…Higher concentrations of voriconazole can also cause host toxicity, including off-target effects on host sterol synthesis and homeostasis (43,44), and can have adverse effects in patients, such as cutaneous malignancy, photosensitivity, and periostitis (45). Some triazole drugs are toxic to zebrafish, especially during development (46)(47)(48), and there may be some toxicity associated with higher doses of voriconazole (10 g/ml) in immunosuppressed zebrafish. However, we do not find any toxicity of voriconazole at the dose at which we performed the bulk of our experiments (1 g/ml).…”

Section: Figmentioning

confidence: 99%

Efficacy of Voriconazole against Aspergillus fumigatus Infection Depends on Host Immune Function

Rosowski

Huisken

et al. 2020

Antimicrob Agents Chemother

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Antifungal therapy can fail in a remarkable number of patients with invasive fungal disease, resulting in significant morbidity worldwide. A major contributor to this failure is that while these drugs have high potency in vitro, we do not fully understand how they work inside infected hosts. Here, we used a transparent larval zebrafish model of Aspergillus fumigatus infection amenable to real-time imaging of invasive disease as an in vivo intermediate vertebrate model to investigate the efficacy and mechanism of the antifungal drug voriconazole. We found that the ability of voriconazole to protect against A. fumigatus infection depends on host innate immune cells and, specifically, on the presence of macrophages. While voriconazole inhibits fungal spore germination and growth in vitro, it does not do so in larval zebrafish. Instead, live imaging of whole, intact larvae over a multiday course of infection revealed that macrophages slow down initial fungal growth, allowing voriconazole time to target and kill A. fumigatus hyphae postgermination. These findings shed light on how antifungal drugs such as voriconazole may synergize with the immune response in living hosts.

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Section: Figmentioning

confidence: 99%

Efficacy of Voriconazole against Aspergillus fumigatus Infection Depends on Host Immune Function

Rosowski

Huisken

et al. 2020

Antimicrob Agents Chemother

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“…This antifungal also produced teratogenic effects in rat embryos by interfering with the cellular and molecular mechanisms that control NCCs migration (Di Renzo, Broccia, Giavini, & Menegola, ; Menegola, Broccia, Di Renzo, & Giavini, ) and changed the level of expression of the NC‐related genes xCRABP , Hoxa2 and Xbap in Xenopus tadpoles (Papis, Bernardini, Gornati, Menegola, & Prati, ). In zebrafish embryos, cyproconazole, flusilazole, and fenbuconazole exposure resulted in a shortening of the NC‐derived Meckel's and ceratohyal cartilages (Staal et al, ; Wu et al, ), while propiconazole decreased larval survival and induced hypopigmentation (Souders et al, ). Therefore, the effects of such fungicides have long‐term consequences on the development of NC derivatives in vertebrate embryos.…”

Section: Chemical Agentsmentioning

confidence: 99%

The role of teratogens in neural crest development

Cerrizuela

Vega-Lopez

Aybar

2020

Birth Defects Research

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The neural crest (NC), discovered by Wilhelm His 150 years ago, gives rise to a multipotent migratory embryonic cell population that generates a remarkably diverse and important array of cell types during the development of the vertebrate embryo. These cells originate in the neural plate border (NPB), which is the ectoderm between the neural plate and the epidermis. They give rise to the neurons and glia of the peripheral nervous system, melanocytes, chondrocytes, smooth muscle cells, odontoblasts and neuroendocrine cells, among others. Neurocristopathies are a class of congenital diseases resulting from the abnormal induction, specification, migration, differentiation or death of NC cells (NCCs) during embryonic development and have an important medical and societal impact. In general, congenital defects affect an appreciable percentage of newborns worldwide. Some of these defects are caused by teratogens, which are agents that negatively impact the formation of tissues and organs during development. In this review, we will discuss the teratogens linked to the development of many birth defects, with a strong focus on those that specifically affect the development of the NC, thereby producing neurocristopathies.Although increasing attention is being paid to the effect of teratogens on embryonic development in general, there is a strong need to critically evaluate the specific role of these agents in NC development. Therefore, increased understanding of the role of these factors in NC development will contribute to the planning of strategies aimed at the prevention and treatment of human neurocristopathies, whose etiology was previously not considered.

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“…In the hazard classification, propiconazole is classified as reproductive toxicity 1B category and belongs to endocrine disrupting substances [4]. Related studies have confirmed that propiconazole can result in genetic toxicity, liver toxicity and growth toxicity [5][6][7]. The U.S. Environmental Protection Agency has included propiconazole in the list of possible human carcinogens [8].…”

Section: Introductionmentioning

confidence: 99%

Antibody Generation and Rapid Immunochromatography Using Time-Resolved Fluorescence Microspheres for Propiconazole: Fungicide Abused as Growth Regulator in Vegetable

Chen

Shen

et al. 2022

Foods

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Propiconazole (PCZ) is a fungicide popularly used to prevent and control wheat and rice bakanae disease, etc. However, it was recently found to be illegally employed as a plant regulator to induce thick stems and dark green leaves of Brassica campestris, a famous vegetable in Guangdong, South China. Due to a lack of available recognition molecules to the target analyte, it is still a big challenge to establish a rapid surveillance screening method. In this study, a novel chiral hapten was rationally designed, and an artificial immunogen was then prepared for the generation of a specific antibody against propiconazole for the first time. Using the obtained antibody, a highly sensitive time-resolved fluorescence microspheres lateral flow immunochromatographic assay (TRFMs-LFIA) was established with a visual limit of detection of 100 ng/mL and a quantitative limit of detection of 1.92 ng/mL for propiconazole. TRFMs-LFIA also exhibited good recoveries ranging from 78.6% to 110.7% with coefficients of variation below 16%. The analysis of blind real-life samples showed a good agreement with results obtained using HPLC-MS/MS. Therefore, the proposed method could be used as an ideal screening surveillance tool for the detection of propiconazole in vegetables.

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Sub-lethal effects of the triazole fungicide propiconazole on zebrafish (Danio rerio) development, oxidative respiration, and larval locomotor activity

Cited by 59 publications

References 46 publications

Efficacy of Voriconazole against Aspergillus fumigatus Infection Depends on Host Immune Function

Efficacy of Voriconazole against Aspergillus fumigatus Infection Depends on Host Immune Function

The role of teratogens in neural crest development

Antibody Generation and Rapid Immunochromatography Using Time-Resolved Fluorescence Microspheres for Propiconazole: Fungicide Abused as Growth Regulator in Vegetable

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