Sub-lethal hyperthermia promotes epithelial-to-mesenchymal-like transition of breast cancer cells: implication of the synergy between hyperthermia and chemotherapy

Lee, Tae Hee; Bu, Jong‐Uk; Kim, Byoung Hyuck; Poellmann, Michael J.; Hong, Seungpyo; Hyun, Sung Hee

doi:10.1039/c8ra08472f

Cited by 27 publications

(21 citation statements)

References 25 publications

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“…Human breast cancer cell line, MCF-7, was treated with cytochalasin B (CB, Tocris Biosciences, MO) at varying concentration of 0, 5, 10, 15, and 20 μg/mL and for varying incubation time of 0.5, 3, and 24 h, respectively. Cell viability was measured using two-colored live/dead cell viability assay kit (Life Technology, CA) and AlamarBlue (Thermo Scientific, IL) as described elsewhere [24, 25]. See Additional file 1 for additional details.…”

Section: Methodsmentioning

confidence: 99%

“…We compared the expressions of two cancer-related markers, epidermal growth factor receptor (EGFR) or/and epithelial cell adhesion molecule (EpCAM), based on immunocytochemistry (ICC) and immunofluorescence (IF) assays. ICC was performed directly to the CB-treated cells loaded on the fabric filters using the antibody against human EGFR (R&D Systems, MN), as described previously [25]. IF was conducted after retrieving the captured cancer cells from the fabric filters and immobilizing them on the microscope slides.…”

Section: Methodsmentioning

confidence: 99%

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Multi-modal liquid biopsy platform for cancer screening: screening both cancer-associated rare cells and cancer cell-derived vesicles on the fabric filters for a reliable liquid biopsy analysis

Shim

Lee

et al. 2019

Nano Convergence

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Circulating tumor cells (CTCs) are receiving a great amount of scientific interest as a diagnostic biomarker for various types of cancer. Despite the recent progress in the development of highly sensitive CTC isolation devices, post-capture analysis of CTCs is still hindered by technical challenges associated with their rarity. Herein, we present a multi-modal CTC screening platform which is capable to analyze CTCs and CTC-derived extracellular vesicles (EVs), simultaneously from a single sample. Cytochalasin B (CB) treatment promotes cells to release large number of EVs from their surface, as demonstrated by CB-treated cells (5 µg/mL for 3 h) secreting 3.5-fold more EVs, compared to the non-treated cells. CB further generates 1.7-fold more EVs from the cells captured on our CTC filtration device (the fabric filter), compared to those from the cell culture flasks, owing to its multiple pore structure design which reduces the non-specific binding of EVs. Both CB-treated cancer cells and CB-induced EVs are found to overexpress tumor-associated markers, demonstrating a potential for the development of CTC dual-screening platform. Collectively, the results presented in this study reveal that our multi-modal cancer screening platform can synergistically improve the reliability and efficacy of the current CTC analysis systems.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Multi-modal liquid biopsy platform for cancer screening: screening both cancer-associated rare cells and cancer cell-derived vesicles on the fabric filters for a reliable liquid biopsy analysis

Shim

Lee

et al. 2019

Nano Convergence

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“…In HCC, several studies have found that insufficient ablation enhances the invasion and migration abilities of residual tumors. Some have found that sublethal thermal stimulus induced by insufficient ablation can induce EMT in residual HCC cells and breast cancer cells [15,16,18,29]. Other studies have also found that sublethal thermal stimulus lead to the differentiation of viable HCC cells into cancer stem cells [11,[30][31][32].…”

Section: Discussionmentioning

confidence: 99%

“…We believed that 44 C is the temperature with the most malignant characteristic. In HCC, the malignant behavior of HCC cells could be enhanced at 47 C [17,29,[31][32][33][34][35]. It has been proved that temperatures which induced an increase in malignant behavior were difference in different tumors.…”

Section: Discussionmentioning

confidence: 99%

Insufficient ablation promotes the metastasis of residual non-small cell lung cancer (NSCLC) cells via upregulating carboxypeptidase A4

Yan

Lyu

Wang³

et al. 2021

International Journal of Hyperthermia

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Background: Thermal ablation is a potentially curative therapy for early-stage non-small cell lung cancer (NSCLC). Early recurrence after thermal ablation necessitates our attention. Methods: The invasion and migration abilities of NSCLC after sublethal heat stimulus were observed in vitro and in vivo. Sublethal thermal stimulus molecular changes were identified by RNA sequencing. A xenograft model of NSCLC with insufficient ablation was established to explore the epithelial-tomesenchymal transition (EMT) and metastasis-related phenotypes alteration of residual tumors. Results: In vitro, the invasion and migration abilities of NSCLC cells were enhanced 72 h after 44 C and 46 C thermal stimulus. Epithelial-mesenchymal transition (EMT) phenotypes were also upregulated under these conditions. RNA sequencing revealed that the expression of carboxypeptidase A4 (CPA4) was significantly upregulated after thermal stimulus. Significant upregulation of CPA4 and EMT phenotypes was also found in the xenograft model of insufficient NSCLC ablation. The EMT process and invasion and migration abilities can be reversed by silencing CPA4. Conclusions: This study demonstrates that sublethal heat stimulus caused by insufficient ablation can promote EMT and enhance the metastatic capacity of NSCLC. CPA4 plays an important role in these biological processes. Inhibition of CPA4 might be of great significance for improving early-stage NSCLC survival after ablation.

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“…Hyperthermal stress was induced increasing the incubator temperature to 43 °C as previous reported in [46]. After 45 min of exposure, cells were reported at temperature of 37 °C for 12 h. In this way, the assays reported and the confocal images were conducted in heat treated cells and without heat exposure.…”

Section: Methodsmentioning

confidence: 99%

Encapsulation of Thermo-Sensitive Lauric Acid in Silica Shell: A Green Derivate for Chemo-Thermal Therapy in Breast Cancer Cell

et al. 2019

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Lauric acid is a green derivate that is abundant in some seeds such as coconut oil where it represents the most relevant fatty acid. Some studies have emphasized its anticancer effect due to apoptosis induction. In addition, the lauric acid is a Phase Change Material having a melting temperature of about 43.2 °C: this property makes it a powerful tool in cancer treatment by hyperthermal stress, generally induced at 43 °C. However, the direct use of lauric acid can have some controversial effects, and it can undergo degradation phenomena in the extracellular environment. For this reason, we have encapsulated lauric acid in a silica shell with a one-step and reproducible synthetic route in order to obtain a monodispersed SiO2@LA NPs with a good encapsulation efficiency. We have used these NPs to expose breast cancer cell lines (MCF-7) at different concentrations in combination with hyperthermal treatment. Uptake, viability, oxidative stress induction, caspases levels, and morphometric parameters were analyzed. These nanovectors showed double action in anticancer treatments thanks to the synergic effect of temperature and lauric acid activity.

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Sub-lethal hyperthermia promotes epithelial-to-mesenchymal-like transition of breast cancer cells: implication of the synergy between hyperthermia and chemotherapy

Abstract: Thermotherapy has demonstrated a potential to be an effective non-surgical technique to treat breast cancer.

Cited by 27 publications

References 25 publications

Multi-modal liquid biopsy platform for cancer screening: screening both cancer-associated rare cells and cancer cell-derived vesicles on the fabric filters for a reliable liquid biopsy analysis

Multi-modal liquid biopsy platform for cancer screening: screening both cancer-associated rare cells and cancer cell-derived vesicles on the fabric filters for a reliable liquid biopsy analysis

Insufficient ablation promotes the metastasis of residual non-small cell lung cancer (NSCLC) cells via upregulating carboxypeptidase A4

Encapsulation of Thermo-Sensitive Lauric Acid in Silica Shell: A Green Derivate for Chemo-Thermal Therapy in Breast Cancer Cell

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