Sub-micrometer Patterning of Proteins by Electric Lithography

Chang, Yu Cheng; Ahn, Yong Sik; Hahn, H. Thomas; Chen, Yong

doi:10.1021/la063646z

Cited by 16 publications

(25 citation statements)

References 25 publications

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“…A novel electrochemical lithography technique was used to create patterns of biotin by local, heat-assisted release of surface protecting groups. [220] Other approaches made use of soft or probe lithography, either for direct local delivery of biotin or for creating suitable reactive patches that subsequently react with biotin derivatives. [124,199,[220][221][222][223][224][225][226][227][228][229][230] The dimensions of the fabricated SAv patterns range from micrometers to a few tens of nanometers; some of these patterns have been used for the immobilization of fluorescent proteins and antibodies.…”

Section: Namementioning

confidence: 99%

Chemical Strategies for Generating Protein Biochips

et al. 2008

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Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.

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Section: Namementioning

confidence: 99%

Chemical Strategies for Generating Protein Biochips

et al. 2008

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“…Templates have been fabricated through controlling the placement of whole ECM molecules (or moieties present in the ECM to which integrins bind) on the surface. These nano-or 30 electron beam lithography, 31 and selfassembly. 32 As an example of self-assembly, a patterning method using block copolymer nanolithography (BCN) has been developed recently.…”

Section: Introductionmentioning

confidence: 99%

Cell adhesion on nanopatterned fibronectin substrates

et al. 2010

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“…43–49 One common technique exploits a photolithographic resist to protect regions of the surface during aninitial functionalization. Upon removal of the resist, theopen regions are backfilled with a second functionalityby immersion.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Surfaces with Discrete Functionalized Regions for Biological Applications

et al. 2008

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In this paper we describe a method for creating multifunctional glass surfaces presenting discrete patches of different proteins on an inert PEG-functionalized background. Microcontact printing is used to stamp the substrate with octadecyltrichlorosilane to define the active regions. The substrate is then back-filled with PEG-silane {[[2-methoxypoly(ethyleneoxy)]propyl]trimethoxysilane} to define passive regions. A microfluidics device is subsequently affixed to the substrate to deliver proteins to the active regions, with as many channels as there are proteins to be patterned. Examples of trifunctional surfaces are given which present three terminating functional groups, i.e., protein 1, protein 2, and PEG. These surfaces should be broadly useful in biological studies, as patch size is well established to influence cell viability, growth, and differentiation. Three examples of cellular interactions with the surfaces are demonstrated, including the capture of cells from a single cell suspension, the selective sorting of cells from a mixed suspension, and the adhesion of cells to ligand micropatches at critical shear stresses. Within these examples, we demonstrate that the patterned immobilized proteins are active, as they retain their ability to interact with either antibodies in solution or receptors presented by cells. When appropriate (e.g., for E-selectin), proteins are patterned in their physiological orientations using a sandwich immobilization technique, which is readily accommodated within our method. The protein surface densities are highly reproducible in the patches, as supported by fluorescence intensity measurements. Potential applications include biosensors based on the interaction of cells or of marker proteins with protein patches, fundamental studies of cell adhesion as a function of patch size and shear stress, and studies of cell differentiation as a function of surface cues.

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Sub-micrometer Patterning of Proteins by Electric Lithography

Cited by 16 publications

References 25 publications

Chemical Strategies for Generating Protein Biochips

Chemical Strategies for Generating Protein Biochips

Cell adhesion on nanopatterned fibronectin substrates

Multifunctional Surfaces with Discrete Functionalized Regions for Biological Applications

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