Sub-proteomic study on macrophage response to Candida albicans unravels new proteins involved in the host defense against the fungus

Reales-Calderón, José Antonio; Martínez-Solano, Laura; Martínez-Gomáriz, Montserrat; Nombela, César; Molero, Gloria; Gil, Concha

doi:10.1016/j.jprot.2012.01.037

Cited by 21 publications

(26 citation statements)

References 71 publications

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“…With regard to fungi, proteomic analyses of the interaction of pathogenic fungi with macrophages have mainly been studied in C. albicans [96,97,98,99,100]. The first proteomics study on C. albicans -macrophage interaction was performed by Fernández-Arenas et al [99].…”

Section: Interaction Of Macrophages With Human Pathogenic Fungimentioning

confidence: 99%

“…Differentially regulated proteins during the interaction of macrophages were involved in immune, pro-inflammatory and oxidative responses, the unfolded protein response, and apoptosis. Interestingly, for the PRR Galectin-3, not only an increase in abundance, but also a distinct allocation along the interaction was observed, namely more Galectin-1 was found extracellularly [98]. Later, the same group investigated the phosphoproteome of RAW 264.7 macrophages in response to co-incubation with C. albicans .…”

Section: Interaction Of Macrophages With Human Pathogenic Fungimentioning

confidence: 99%

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Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

et al. 2015

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Opportunistic human pathogenic fungi including the saprotrophic mold Aspergillus fumigatus and the human commensal Candida albicans can cause severe fungal infections in immunocompromised or critically ill patients. The first line of defense against opportunistic fungal pathogens is the innate immune system. Phagocytes such as macrophages, neutrophils and dendritic cells are an important pillar of the innate immune response and have evolved versatile defense strategies against microbial pathogens. On the other hand, human-pathogenic fungi have sophisticated virulence strategies to counteract the innate immune defense. In this context, proteomic approaches can provide deeper insights into the molecular mechanisms of the interaction of host immune cells with fungal pathogens. This is crucial for the identification of both diagnostic biomarkers for fungal infections and therapeutic targets. Studying host-fungal interactions at the protein level is a challenging endeavor, yet there are few studies that have been undertaken. This review draws attention to proteomic techniques and their application to fungal pathogens and to challenges, difficulties, and limitations that may arise in the course of simultaneous dual proteome analysis of host immune cells interacting with diverse morphotypes of fungal pathogens. On this basis, we discuss strategies to overcome these multifaceted experimental and analytical challenges including the viability of immune cells during co-cultivation, the increased and heterogeneous protein complexity of the host proteome dynamically interacting with the fungal proteome, and the demands on normalization strategies in terms of relative quantitative proteome analysis.

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Section: Interaction Of Macrophages With Human Pathogenic Fungimentioning

confidence: 99%

Section: Interaction Of Macrophages With Human Pathogenic Fungimentioning

confidence: 99%

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

et al. 2015

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“…Using genome data, we could distinguish each peptides derived from the individual organisms by referring individual genome sequences. Omitting the individual cell isolation steps is important because these steps are known to alter the natural states of protein networks by causing various artifacts from unnecessary stresses (Reales-Calderon et al 2012 ; Rupp 2004 ; Fernandez-Arenas et al 2007 ; Reales-Calderon et al 2013 ). This is the first report of a mixed and quantitative proteome analysis in which the purification and fractionation processes were completely omitted.…”

Section: Introductionmentioning

confidence: 99%

Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation

et al. 2015

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Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In this study, native interactions of C. albicans with macrophages were investigated by proteome analysis using high efficiency of long monolithic silica capillary column. Using this system, we developed a method of “mixed and quantitative proteome analysis” in which C. albicans and macrophages were simultaneously analyzed by nanoLC–MS/MS without the need to isolate the two individual living cells. Two hundred twenty-seven proteins from C. albicans and five proteins from macrophages were identified as candidate interaction-specific molecules. C. albicans seemed to produce glucose through a β-oxidation pathway, a glyoxylate cycle, and gluconeogenesis for escape from macrophages. Up-regulation of stress-related and candidate pathogenic proteins in C. albicans indicated how C. albicans endured the harsh environment inside the macrophages. Down-regulation of apoptosis-associated protein NOA1- and chaperone HSPA1A-syntheses in macrophage indicated that C. albicans was able to escape from macrophages in part by suppressing the production of these macrophage proteins.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-015-0127-2) contains supplementary material, which is available to authorized users.

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“…One of the most useful techniques to study changes in proteomes with different aims [18] is the two-dimensional difference gel electrophoresis (2D-DIGE) [19]. This technology is based on the labelling of a mixture composed by up to three protein samples that can be labelled with 3 different fluorescent dyes, Cy2, Cy3 and Cy5 in the same 2-DE gel.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence

Buján

Hernández-Haro

Monteoliva

et al. 2015

Journal of Proteomics

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Edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the Norwest of Spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. One hundred and three proteins that presented different abundance were successfully identified and classified into 11 functional categories according to their biological processes: amino acid, carbohydrate and lipid metabolism, tricarboxylic cycle, stress response and protein fate, protein synthesis, biogenesis of cellular components, cell rescue defence and virulence, cell membrane and transport, signal transduction and purine and pyrimidine metabolism. Twenty three protein spots detected only in turbot isolate were identified. It was shown that the same proteins appeared in different spots in the two isolates. Mass spectra obtained by MALDITOF/TOF of some of these proteins and DNA sequencing explained the changes as a result of different amino acid sequences. Several proteins related with the virulence of E. tarda (FliC, ArnA or FeSODI) were only detected in the turbot European isolate.

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Sub-proteomic study on macrophage response to Candida albicans unravels new proteins involved in the host defense against the fungus

Cited by 21 publications

References 71 publications

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation

Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence

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