Sub-specific differentiation of intestinal spirochaete isolates by macrorestriction fragment profiling

Rayment, Sarah J.; Livesley, M. A.; Barrett, Stephen

doi:10.1099/00221287-143-9-2923

Cited by 16 publications

(12 citation statements)

References 27 publications

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“…Digestion of DNA with MluI gave 9-16 DNA bands. Using this enzyme, 37 distinct banding patterns were obtained for the 39 isolates plus 6 control isolates, confirming previous observations that B. pilosicoli is genetically heterogeneous [17][18][19]. Isolates from Aboriginals, migrants and control strains were widely distributed throughout the dendrogram produced from analysis of MluI patterns (not shown).…”

supporting

confidence: 68%

“…After analysis by SmaI, WHP02, WHP08 and WHP09 demonstrated minor departures from the main pattern exhibited by the other three isolates. Previous PFGE studies of human and animal strains have found multiple isolates within the same type to be linked geographically [17][18][19], but this is the first report of a widely disseminated strain or cluster of strains approximating a clonal group.…”

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confidence: 94%

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Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Brooke

Clair²,

Mikosza

et al. 2001

Epidemiol. Infect.

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SUMMARYThe purpose of this study was to investigate carriage of intestinal spirochaetes by selected population groups in Western Australia. Stool specimens from 293 rural patients with gastrointestinal disorders, and from 227 healthy migrants from developing countries were cultured. Spirochaete isolates were identified using PCR, and typed by pulsed field gel electrophoresis (PFGE). Brachyspira aalborgi was not isolated. Brachyspira pilosicoli was recovered from 15 rural patients, all Aboriginal. Prevalence was 9n9 % in 151 Aboriginals and 0 % in 142 non-Aboriginals. Carriage of B. pilosicoli amongst migrants was 10n6 % (24\227). Carriage was significantly increased in Aboriginal children aged 2-5 years (P l 0n0027) and in migrant individuals from the Middle East and Africa (P l 0n0034). Carriage was significantly associated with detection of faecal protozoa in both Aboriginals (P l 0n0021) and migrants (P l 0n012). PFGE results indicated that the B. pilosicoli strains were genetically diverse.

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confidence: 68%

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confidence: 94%

Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Brooke

Clair²,

Mikosza

et al. 2001

Epidemiol. Infect.

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“…Infection in other groups is apparently negligible (Tompkins et al, 1981(Tompkins et al, , 1986Lee & Hampson, 1992). Studies based on genetic (Lee et al, 1993a, b;Lee & Hampson, 1994;Atyeo et al, 1996;Rayment et al, 1997) and phenotypical (Barrett et al, 1996) analyses of S. pilosicoli strains have shown these to be diverse. Most of these studies were performed on relatively small numbers of isolates from diverse geographical regions and populations with little opportunity for transmission to occur between them.…”

Section: Introductionmentioning

confidence: 99%

Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea

Trott

Mikosza²,

Combs³

et al. 1998

International Journal of Systematic Bacteriology

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The population genetics of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands province of Papua New Guinea were investigated. Multilocus enzyme electrophoresis (MLEE) was used to analyse 164 isolates from humans and animals. These were divided into 33 electrophoretic types (ETs), four of which contained 65% of the isolates. The mean genetic diversity (n = number of ETs) for 145 human isolates was 0.18, and the mean number of alleles at five polymorphic loci was 2.6. The species appeared to be recombinant, as there was a lack of linkage disequilibrium, and 25% of all the possible combinations of alleles was present in the population. PFGE analysis using the enzymes MluI and Sall divided 157 of the isolates into 99 PFGE types, demonstrating the existence of considerable strain diversity in a geographically restricted area. The two techniques were in excellent agreement; however, PFGE was more discriminatory for strain typing than was MLEE. Nine out of 19 (474%) culture-positive individuals were colonized by the same PFGE type of 5. pilosicoli when retested after 6 weeks. For three individuals, the PFGE profiles of the second isolate differed from the first in only one or two DNA bands, while the other seven individuals were colonized with distinct PFGE types on each occasion. In two cases, strains with the same PFGE pattern were isolated from humans and dogs, suggesting that crossspecies transmission of 5. pilosicoli may occur naturally and that the infection can be zoonotic.

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“…The phylogeny of human intestinal spirochaetes has been studied using multilocus enzyme electrophoresis (MEE) (Lee & Hampson, 1994) Barrett 1996) and PFGE (Atyeo et al, 1996;Rayment et al, 1997). These techniques revealed considerable heterogeneity amongst human isolates, but the isolates fell into a distinct group (Lee & Hampson, 1994;Barrett et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Variation amongst human isolates of Brachyspira (Serpulina) pilosicoli based on biochemical characterization and 16S rRNA gene sequencing

Smet¹,

Worth²,

Barrett³

1998

International Journal of Systematic Bacteriology

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Brachyspira pilosicoli (formerly Serpulina pilosicoli) causes swine spirochaetosis and can also be isolated from human faeces, although its role in human disease remains unclear. The genetic and biochemical variations amongst 19 isolates of human spirochaetes from five different countries were evaluated and compared to those found amongst swine isolates of B. pilosicoli. All isolates were negative for P-glucosidase and all but one were positive for hippurate hydrolysis, which are characteristics typical of B. pilosicoli. The isolates showed variation in indole production and agalactosidase and a-glucosidase activity, other characteristics which can be used to identify B. pilosicoli. The DNA sequences of part of the 165 rRNA gene differed from each other and from that of B. pilosicoli by 0-3 bp out of 283 bp.It is concluded that there is considerable variation amongst human intestinal spirochaetes. Since few of the isolates reported here match the current criteria for B. pilosicoli, it is concluded that this species is more heterogeneous than previously appreciated. However, it cannot be excluded that some isolates may belong to uncharacterized related BrachyspiralSerpulina species.

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Sub-specific differentiation of intestinal spirochaete isolates by macrorestriction fragment profiling

Cited by 16 publications

References 27 publications

Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia

Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea

Variation amongst human isolates of Brachyspira (Serpulina) pilosicoli based on biochemical characterization and 16S rRNA gene sequencing

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