Sub-toxic effects of CuO nanoparticles on bacteria: Kinetics, role of Cu ions and possible mechanisms of action

Bondarenko, Olesja; Ivask, Angela; Käkinen, Aleksandr; Kahru, Anne

doi:10.1016/j.envpol.2012.05.009

Cited by 191 publications

(127 citation statements)

References 38 publications

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“…Bondarenko in 2012 showed that Cu Oxide nano particles react with Amin and Carboxyl groups on the surface of microbial cells and release Cu in Oxidation reactions which leads to antibacterial effects by releasing Hydroxyl radicals ( Bodarenko et al, 2012). Sondi in 2004 showed that nano-particles have a reductive effect during DNA replication process (Sondi et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR

Arab

Sadi

Amini

2018

J Hellenic Vet Med Soc

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Increasing bacterial resistance towards traditional/conventional antibiotics is a major global health concern worldwide. Iron oxide nanoparticles (Fe nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. The aim of this work was to investigate the antibacterial effects and mechanism of action of iron nanoparticles against the expression of the tetA gene in Tetracycline Resistant Staphylococcus aureus strains by real time PCR. In the cross-sectional study, a total of 60 S. aureus were collected. Antibiotic susceptibility test was performed on the muller hinton agar according to the Clinical and Laboratory Standards Institute (CLSI). Then all strains were evaluated for tetA, tetB, tetC and tetD genes by multiplex-PCR method. In-vitro activity of iron oxide nanoparticles was evaluated against all resistant strains by microbroth dilution method. Therefore, the expression of tetA gene was measured in treated with iron oxide nanoparticles and untreated resistant S. aureus strain by Real time PCR. Our results indicated 25 (41.66%) strains resistant to Tetracycline. The prevalence of tetA, tetB, tetC and tetD genes were 5 (8.33%), 2 (2.33%), 20 (33.33%) and 10 (10.67%), respectively. The expression of tetA genes in resistant S. aureus strains treated with Iron oxide nanoparticles was lower than the untreated isolates. Iron oxide nanoparticles have strong antibacterial activity against resistant to Tetracycline S. aureus strains. In addition to, these nanoparticles reduce the expression of antibiotic resistance gene.

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Section: Discussionmentioning

confidence: 99%

Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR

Arab

Sadi

Amini

2018

J Hellenic Vet Med Soc

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“…Many studies have suggested that dissolution is also an important factor which influences the harmful effects of CuO NPs in biological systems (Aruoja et al, 2009;Bondarenko et al, 2012;Heinlaan et al, 2008;Kahru & Dubourguier, 2010;Kasemets et al, 2013). In our previous studies, we have proposed that solubility-dependent toxicity of CuO NPs is prevalent especially in case of unicellular organisms surrounded by rigid cell wall.…”

Section: Toxicity Of Ag Zno and Cuo Nanoparticles Due To Dissolutionmentioning

confidence: 99%

Mechanisms of toxic action of Ag, ZnO and CuO nanoparticles to selected ecotoxicological test organisms and mammalian cells in vitro: A comparative review

et al. 2013

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Silver, ZnO and CuO nanoparticles (NPs) are increasingly used as biocides. There is however increasing evidence of their threat to ''non-target'' organisms. In such a context, the understanding of the toxicity mechanisms is crucial for both the design of more efficient nanoantimicrobials, i.e. for ''toxic by design'' and at the same time for the design of nanomaterials that are biologically and/or environmentally benign throughout their life-cycle (safe by design). This review provides a comprehensive and critical literature overview on Ag, ZnO and CuO NPs' toxicity mechanisms on the basis of various environmentally relevant test species and mammalian cells in vitro. In addition, factors modifying the toxic effect of nanoparticles, e.g. impact of the test media, are discussed. Literature analysis revealed three major phenomena driving the toxicity of these nanoparticles: (i) dissolution of nanoparticles, (ii) organismdependent cellular uptake of NPs and (iii) induction of oxidative stress and consequent cellular damages. The emerging information on quantitative structure-activity relationship modeling of nanomaterials' toxic effects and the challenges of extrapolation of laboratory results to the environment are also addressed.

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“…[1][2][3][4][5][6][7][8][9][10] Although in the vast nanotoxicological literature of the last decade studies concerned with the assessment of metal or metal-oxide NP toxicity are not at the top of the list, such publications are nevertheless quite numerous. To illustrate this, we may refer to several works devoted to NPs of the metals that were the subject-matter of our own studies considered below: iron oxide, [11][12][13][14][15][16][17][18][19][20] silver, gold, 24,[45][46][47][48][49][50][51][52][53][54][55][56][57][58] copper and copper oxide, 43,[59][60][61][62][63][64][65][66][67][68] nickel oxide, 65,[69]…”

mentioning

confidence: 99%

Some inferences from in vivo experiments with metal and metal oxide nanoparticles: the pulmonary phagocytosis response, subchronic systemic toxicity and genotoxicity, regulatory proposals, searching for bioprotectors (a self-overview)

Katsnelson¹,

Privalova²,

Sutunkova³

et al. 2015

IJN

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The purpose of this paper is to overview and summarize previously published results of our experiments on white rats exposed to either a single intratracheal instillation or repeated intraperitoneal injections of silver, gold, iron oxide, copper oxide, nickel oxide, and manganese oxide nanoparticles (NPs) in stable water suspensions without any chemical additives. Based on these results and some corroborating data of other researchers we maintain that these NPs are much more noxious on both cellular and systemic levels as compared with their 1 μm or even submicron counterparts. However, within the nanometer range the dependence of systemic toxicity on particle size is intricate and non-unique due to complex and often contra-directional relationships between the intrinsic biological aggressiveness of the specific NPs, on the one hand, and complex mechanisms that control their biokinetics, on the other. Our data testify to the high activity of the pulmonary phagocytosis of NPs deposited in airways. This fact suggests that safe levels of exposure to airborne NPs are possible in principle. However, there are no reliable foundations for establishing different permissible exposure levels for particles of different size within the nanometric range. For workroom air, such permissible exposure levels of metallic NP can be proposed at this stage, even if tentatively, based on a sufficiently conservative approach of decreasing approximately tenfold the exposure limits officially established for respective micro-scale industrial aerosols. It was shown that against the background of adequately composed combinations of some bioactive agents (comprising pectin, multivitamin-multimineral preparations, some amino acids, and omega-3 polyunsaturated fatty acid) the systemic toxicity and even genotoxicity of metallic NPs could be markedly attenuated. Therefore we believe that, along with decreasing NP-exposures, enhancing organisms’ resistance to their adverse action with the help of such bioprotectors can prove an efficient auxiliary tool of health risk management in occupations connected with them.

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Sub-toxic effects of CuO nanoparticles on bacteria: Kinetics, role of Cu ions and possible mechanisms of action

Cited by 191 publications

References 38 publications

Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR

Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR

Mechanisms of toxic action of Ag, ZnO and CuO nanoparticles to selected ecotoxicological test organisms and mammalian cells in vitro: A comparative review

Some inferences from in vivo experiments with metal and metal oxide nanoparticles: the pulmonary phagocytosis response, subchronic systemic toxicity and genotoxicity, regulatory proposals, searching for bioprotectors (a self-overview)

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