Sub-Zero Preservation of Atlantic Salmon Sperm

Truscott, B.; Idler, D. R.; Hoyle, R. J.; Freeman, H. C.

doi:10.1139/f68-028

Cited by 70 publications

(25 citation statements)

References 9 publications

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“…The possible precautions that could prolong the sperm viability may include: (i) the prevention of desiccation (especially important in the case of storage in capped containers) either by using a moisture-securing system (36) or by dilution with immobilizing diluents (28,40); (ii) lowering the storage temperature to below 1 o C, which prevents bacterial growth and stops metabolic processes in spermatozoa (36,40); (iii) optimizing gaseous atmosphere: an oxygen atmosphere can be sub-optimal, as demonstrated by Bencic et al (6); (iv) securing sterility by using sterile dilution media (21) or antibiotics (6,37) to prevent excessive bacterial growth, which was observed in some samples in the present study after 2 weeks of storage; (v) avoid urine contamination (19).…”

Section: Discussionmentioning

confidence: 99%

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Hatipoğlu¹,

Akçay²

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentration and pH), the pooled samples were diluted at a 1:2 ratio with two extenders (0.3 M glucose or Ringer solution). The diluted semen was stored for 48 hours at 4°C. Following the cooled storage, motility of the spermatozoa was evaluated after 24 and 48 hours regarding the post-cooled period. Dry fertilization technique was used for the fertilization trials. Eggs were pooled from 30 females by the hand stripping method. Fertilization took place in dry plastic dishes and 600 eggs were used in each fertilization trial. The sperm-egg ratio was approximately 0.25x10 6 sperm/egg. After swelling, eggs were rinsed with hatchery water (7 o C) and batches were placed into vertical incubation trays. The experimental success was assessed from sperm motility and the percent of eyed-egg 25 days after fertilization. Average fresh semen motility was 81.4 %. According to the results of the experiment, the highest motility (67 % after 24 h, 53 % after 48 h) and movement duration (60 s after 24 h, 42 s after 48 h) were determined by using glucose extender after 24 and 48 hours storage. Fertility rate of fresh semen was 84.4 %. The highest eyed-egg rate (80.4 %) was obtained from semen stored with glucose based extender after 24 h storage. After 48 hour storage fertilization yield for the semen diluted with 0.3 M Glucose decreased to 61.9 % and to 43.8 % for the one diluted with Ringer solution. Our results indicate that glucose based extender is a better preservative than Ringer solution for the short term preservation of Abant trout semen.Key words: Abant trout, semen, short-term preservation, fertilityKısa süreli saklanmış Abant alabalığı spermatozoonlarının fertilizasyon yeteneği Özet: Bu araştırma ile, ülkemize ait endemik bir balık türü olan Abant alabalığının spermatolojik parametrelerinin değerlendirilmesi ve farklı sulandırıcılarda kısa süreli saklanan spermatozoonların fertilizasyon yeteneğinin ortaya konulması amaçlanmıştır. Bu araştırmada, 30 ergin erkek balıktan anestezi yapılmaksızın masaj yoluyla sperma alınmıştır. Birincil spermatolojik parametreler (miktar, motilite, haraket süresi, yoğunluk ve pH) belirlendikten sonra birleştirilen örnekler iki farklı sulandırıcı ile (0,3 M glukoz veya Ringer solusyonu) 1:2 (sperma:sulandırıcı) oranında sulandırılmıştır. Sulandırılan sperma 4°C'de 48 saat saklanmıştır. Saklama işlemi esnasında 24. ve 48. saatlerde motilite değerlendirmesi yapılmıştır. Fertilizasyon denemeleri için kuru fertilizasyon tekniği kullanılmıştır. Yumurtalar 30 ergin dişiden masaj yoluyla alınmıştır. Fertilizasyon kuru plastic kaplarda gerçekleştirilmiş ve her bir fertilizasyon denemesi için 600 yumurta ku...

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Section: Discussionmentioning

confidence: 99%

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Hatipoğlu¹,

Akçay²

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…The standard Cortland® culture medium (Trus-Cott et al, 1968) for fish spermatozoa (per liter: 1.88 g NaCl, 0.23 g CaCl2, 7.2 g KCl, 0.41 g NaH2PO4, 1 g NaHCO3, 0.23 g MgSO4·7 H2O, 1.0 g Glucose, 10% Glycol and 10% Tris Base and prepared to pH 8 at 268mOsm) was used for all manipulation and served as control. Fresh-retrieved semen was diluted 1:3 in the non-activating Cortland® medium with subsequent determination of the motility and concentration by phase-contrast microscopy.…”

Section: Wwwintechopencommentioning

confidence: 99%

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Isachenko¹,

Mallmann²,

Rahimi³

et al. 2012

Current Frontiers in Cryobiology

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“…This was determined by mixing fresh spermatozoa or frozen-thawed sperma¬ tozoa with eggs from the same female fish. The incubation procedure was similar to that described by Truscott, Idler, Hoyle & Freeman (1968). In every test, a minimum of 500 eggs was incubated and the percentage of fertilization was determined.…”

Section: Sperm Assessmentmentioning

confidence: 99%

Cryogenic preservation of fish and mammalian spermatozoa

Mounib¹

1978

Reproduction

133

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Summary. Various combinations of sucrose, reduced glutathione and potassium bicarbonate were tested for the cryogenic preservation of salmon spermatozoa. When a fast freezing procedure was followed, the extender that gave the best results was composed of 1 part of dimethyl sulphoxide (DMSO), as a protective agent, and 7 parts of a medium containing 125 mM-sucrose, 6.50 mM-reduced glutathione and 100 mM-potassium bicarbonate. Salmon and cod spermatozoa were kept frozen in this extender for 1 year. Freezing resulted in a reduction in the number of motile spermatozoa but had no significant effect on the degree of progression of motile spermatozoa or on their fertilizing capacity. When glycerol replaced DMSO in the extender and a slow freezing procedure was followed, similar results were obtained for the spermatozoa of bull or man; although the number of motile spermatozoa was reduced, there was no effect on the progressive motility score.

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Sub-Zero Preservation of Atlantic Salmon Sperm

Cited by 70 publications

References 9 publications

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954)

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Cryogenic preservation of fish and mammalian spermatozoa

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