Suballeles of the ABO Blood Group System in a Japanese Population

Abstract

The nucleotides (nt) at positions 467 and 646 of the ABO blood group system were analyzed in a Japanese population by means of the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. Two types at nt467, tentatively designated ‘Pro’ and ‘Leu’, were found in the common A (=A¹) alleles, and two types at nt646 named ‘T’ and ‘A’ were found in·alleles. The types at nt467 of B and·alleles were Pro and those at nt646 of A and B alleles were T. Therefore, A alleles were divided into A(Leu) and A(Pro) suballeles and·alleles were divided into O(T) and 0(A). The allele frequencies in the present survey were calculated as ABO*A(Pro) = 0.0712, ABO*A(Leu) = 0.2163, ABO*B = 0.1779, ABO*O(T) = 0.2731 and ABO*O(A) = 0.2615. No O² (or O₍₃₎) allele was observed in the population samples. At least five alleles with polymorphic frequencies and 15 genotypes are present in the Japanese sample.

ABO Genotype and the Risk of Gastric Cancer, Atrophic Gastritis, and Helicobacter pylori Infection

“…Allele frequencies of the O, A, and B allele among the noncancer control subjects were 54.64%, 28.33%, and 17.03%, respectively. These frequencies were consistent with information from the HapMap project and previous studies (22,23,28).…”

Genotyping of the ABO blood group system: analysis of nucleotide position 802 by PCR-RFLP and the distribution of ABO genotypes in a German population

“…In the course of ABO genotyping by the PCR-RFLP method for the analysis of seven positions (nps 261, 467, 526, 646,703,796 and 803) [10], we found a variant allele which was suspected to be the 02 allele. We designed a new primer and analysed the nucleotide at lap 802 of this variant allele using the PCR-RFLP method instead of DNA sequencing.…”

“…Genomic DNA was prepared as described elsewhere [13]. Nps 261, 467, 526, 646, 703, 796 and 803 of the ABO gene were analysed by PCR with the primers GA16 (5'-AGAAGCTGAGTGGAGTTCCAGGTG-3'), GA17 (5'-TGATGGCAAACACAGTTAACCC-3'), GA01N (5'-TC-CTGGAGACGGCGGAGAAGCA-3'), GA13 (5'-ACCGACCC-CCCGAAGAACG-3') and GA14 (5'-ACCGACCCCCCGAAGA-ACC-3'), and RFLP with restriction enzymes BstPI, KpnI, BssHII, BanI, HapII, AluI, MvaI, PvuII and MboI, and ABO genotypes were determined as described previously [10,14]. The nucleotide at np 802 was also analysed by PCR-RFLP.…”

“…Improved and simplified methods, based on the method of Lee and Chang [7], for stain analyses were also reported in subsequent papers [8,9]. We previously reported a PCR-RFLP method for the analysis of seven positions (nps 261,467, 526, 646, 703,796 and 803), and common A and O alleles were divided into suballeles called A(Pro), A(Leu), O(T) and O(A) [10]. Recently, a variant O allele 02 [11] or 0(3) [12] was described.…”

Extension and Modification of Kanai’s DNA Isolation Method for a Spectrum of Human Specimens Collected by Invasive and Noninvasive Methods Suitable for Genotyping Studies

“…Hence, PCR RFLP 16 genotyping was chosen to ensure the high quality of DNA acceptable for molecular analysis. ARMS 17 , another method of genotyping that uses allele-specific primers for polymerase chain reaction, also needs good quality, contaminant-free DNA samples.…”