Subcellular and Molecular Distribution of Cadmium in Two Rice Genotypes With Different Levels of Cadmium Accumulation

Yu, Hui; Zuo-xiang, Xiang; Zhu, Yun; Wang, Junli; Zhi-jian, Yang; Yang, Zhongyi

doi:10.1080/01904167.2012.631668

Cited by 31 publications

(11 citation statements)

References 34 publications

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“…This result is consistent with the observation that D62B absorbs more Cd than D83B. Investigation on several rice genotypes in solution cultivations revealed that a larger cell wall fraction of Cd in the roots was associated with lower translocation ratios from the roots to the aboveground parts (Liu et al 2014;Yu et al 2012). Our results were generally in agreement with their results.…”

Section: Adsorption Chelation Compartmentalization and Translocasupporting

confidence: 95%

Cadmium adsorption, chelation and compartmentalization limit root-to-shoot translocation of cadmium in rice (Oryza sativa L.)

Wang

et al. 2017

Environ Sci Pollut Res

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Strategies to reduce cadmium (Cd) in rice grain, below concentrations that represent serious human health concerns, require that the mechanisms of Cd distribution and accumulation within rice plants be established. Here, a comprehensive hydroponic experiment was performed to investigate the differences in the Cd uptake, chelation and compartmentalization between high (D83B) and low (D62B) Cd-accumulation cultivars contrasting in Cd accumulation in order to establish the roles of these processes in limiting Cd translocation from root to shoot. D83B showed 3-fold higher Cd accumulation in the shoots than the cultivar D62B. However, a short-term Cd uptake experiment showed more Cd uptake by D62B than by D83B. The distribution of Cd in roots and shoots differed significantly. D83B translocated 38% of total Cd taken up to the shoots, whereas D62B retained most of the Cd in the roots. D62B had higher amounts of non-protein thiols (NPTs) and glutathione (GSH) than D83B. The NPT and Cd distribution ratio (CDR) in the anionic form in the roots of D62B increased gradually as Cd concentration increased. In D83B, in contrast, levels of CDR in the cationic form increased significantly from 22.10 to 43.37%, while NPT only increased slightly. Furthermore, the percentage of Cd ions retained in thiol-rich peptides, especially in the HMW complexes, was significantly higher in D62B compared with D83B. However, D83B possessed a greater proportion of potentially mobile (cationic) Cd in the roots and showed superior Cd translocation from root to shoot. Taken as a whole, the results presented in this study revealed that Cd chelation, compartmentalization and adsorption contribute to the Cd retention in roots.

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Section: Adsorption Chelation Compartmentalization and Translocasupporting

confidence: 95%

Cadmium adsorption, chelation and compartmentalization limit root-to-shoot translocation of cadmium in rice (Oryza sativa L.)

Wang

et al. 2017

Environ Sci Pollut Res

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“…Former studies on Cd subcellular distribution and translocation were conducted with two rice cultivars in solution cultivations, and the results indicated that larger ratios of Cd in the soluble fraction in the roots were associated with higher ratios of Cd distribution from the roots to the shoots Yu et al 2012). Our results on the relation between Pb subcellular distribution in root and its translocation from root to shoot were generally in agreement with their results on Cd.…”

Section: Variations Among Rice Cultivars In Pb Subcellular Distributisupporting

confidence: 88%

Relationships Between Subcellular Distribution and Translocation and Grain Accumulation of Pb in Different Rice Cultivars

Liu

Mei

Cai

et al. 2015

Water Air Soil Pollut

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To test the hypothesis that lead (Pb) content of rice grain may be related to its transport and subcellular distribution in rice plant, the present study was conducted with six rice cultivars of different types under different soil Pb levels. The results showed that grain Pb concentrations were correlated positively and significantly (P<0.05 or 0.01) with distribution ratios (DRs) of Pb from shoots to ears/grains, but insignificantly (P>0.05) with the DR from roots to shoots. The DR from shoots to ears/grains was correlated positively and significantly (P<0.05 or 0.01) with subcellular distribution ratios (SDRs) of Pb in soluble fraction of shoots, but negatively and significantly (P<0.05 or 0.01) with the SDR in cell wall fraction of shoots. In conclusion, Pb transportation from the shoot to the grain is the key factor in determining Pb content of rice grain. The Pb distributed in soluble fraction of shoot tissue is the key source of Pb for transferring into the grain. The Pb precipitated in cell wall fraction is the key sink of Pb in shoot tissue that restricts the transport of Pb from the shoot to the grain.

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“…Cd–O storage forms have been also interpreted as Cd in the apoplast that binds to cell wall components ( Isaure et al, 2015 ). Extraction-based analysis of Cd compartmentalization in rice shoots revealed that large fractions of Cd in the shoot (>40%) are located in the apoplast ( Yu et al, 2012 ; Siebers et al, 2013 ; Zhang et al, 2014 ). This Cd accumulation in the apoplast may derive from inefficient loading of Cd from the xylem into the symplast in the shoot ( Olsen and Palmgren, 2014 ) that requires membrane influx transporters that are not identified yet ( Wang W. et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…This recycling step includes an organized degradation of organelles and organic compounds, including proteins. Significant fractions of Cd can be present in organelles in rice shoots (5–17%, Yu et al, 2012 ; Siebers et al, 2013 ; Zhang et al, 2014 ) and/or replacing nutrients such as Zn in proteins in plants ( Tang et al, 2014 ). Hence, in our study, the Cd that was bound to S in the shoots at flowering could have been remobilized toward the grains during grain filling ( Kashiwagi et al, 2009 ; Wang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Changes of Cadmium Storage Forms and Isotope Ratios in Rice During Grain Filling

et al. 2021

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Rice poses a major source of the toxic contaminant cadmium (Cd) for humans. Here, we elucidated the role of Cd storage forms (i.e., the chemical Cd speciation) on the dynamics of Cd within rice. In a pot trial, we grew rice on a Cd-contaminated soil in upland conditions and sampled roots and shoots parts at flowering and maturity. Cd concentrations, isotope ratios, Cd speciation (X-ray absorption spectroscopy), and micronutrient concentrations were analyzed. During grain filling, Cd and preferentially light Cd isotopes were strongly retained in roots where the Cd storage form did not change (Cd bound to thiols, Cd–S = 100%). In the same period, no net change of Cd mass occurred in roots and shoots, and the shoots became enriched in heavy isotopes (Δ114/110Cdmaturity–flowering = 0.14 ± 0.04‰). These results are consistent with a sequestration of Cd in root vacuoles that includes strong binding of Cd to thiol containing ligands that favor light isotopes, with a small fraction of Cd strongly enriched in heavy isotopes being transferred to shoots during grain filling. The Cd speciation in the shoots changed from predominantly Cd–S (72%) to Cd bound to O ligands (Cd–O, 80%) during grain filling. Cd–O may represent Cd binding to organic acids in vacuoles and/or binding to cell walls in the apoplast. Despite this change of ligands, which was attributed to plant senescence, Cd was largely immobile in the shoots since only 0.77% of Cd in the shoots were transferred into the grains. Thus, both storage forms (Cd–S and Cd–O) contributed to the retention of Cd in the straw. Cd was mainly bound to S in nodes I and grains (Cd–S > 84%), and these organs were strongly enriched in heavy isotopes compared to straw (Δ114/110Cdgrains/nodes–straw = 0.66–0.72‰) and flag leaves (Δ114/110Cdgrains/nodes–flag leaves = 0.49–0.52‰). Hence, xylem to phloem transfer in the node favors heavy isotopes, and the Cd–S form may persist during the transfer of Cd from node to grain. This study highlights the importance of Cd storage forms during its journey to grain and potentially into the food chain.

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Subcellular and Molecular Distribution of Cadmium in Two Rice Genotypes With Different Levels of Cadmium Accumulation

Cited by 31 publications

References 34 publications

Cadmium adsorption, chelation and compartmentalization limit root-to-shoot translocation of cadmium in rice (Oryza sativa L.)

Cadmium adsorption, chelation and compartmentalization limit root-to-shoot translocation of cadmium in rice (Oryza sativa L.)

Relationships Between Subcellular Distribution and Translocation and Grain Accumulation of Pb in Different Rice Cultivars

Changes of Cadmium Storage Forms and Isotope Ratios in Rice During Grain Filling

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