Subcellular compartmentalization of cadmium, nickel, and lead in Gammarus fossarum: Comparison of methods

Geffard, Alain; Sartelet, Hervé; Garric, Jeanne; Biagianti-Risbourg, S.; Delahaut, Laurence; Geffard, Olivier

doi:10.1016/j.chemosphere.2009.11.051

Cited by 66 publications

(57 citation statements)

References 44 publications

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“…It is known that crustaceans have detoxification mechanisms to prevent to metal toxicity, including gammarids species (Geffard et al 2010). They can synthesize metallothioneins that are low molecular weight proteins with high affinity for metals decreasing their intracellular availability by sequestration and thus, their toxicity (Ahearn et al 2004;Rainbow 2002).…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, gammarids are widely distributed in European freshwaters and known as ecosystem engineers by facilitating the litter decomposition (Schaller et al 2010). Furthermore, efficient accumulations of metals, essential or not, has been reported in G. pulex or G. fassarum (Geffard et al 2010;Lebrun et al 2011). Because metal accumulations in gammarids can induce sublethal alterations at physiological, biochemical and behavioural levels, these amphipods are also suitable candidates for the development of exposure biomarkers (Dedourge-Geffard et al 2009;Felten et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

Lebrun

Perret²,

Geffard

et al. 2012

Ecotoxicology

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Bioaccumulation enables to integrate the ability of aquatic organisms to regulate metals and effects of water chemistry on metal bioavailability. Linking this process to biological responses offers thus promising lines of enquiry for protecting aquatic ecosystems. This study aims at characterizing the mechanisms involved in waterborne Cu bioaccumulation and assessing metal impact on digestive metabolism in an ecosystem engineer widely distributed in Europe, Gammarus pulex. The organism was exposed to several Cu concentrations (from 0.5 to 100 μg/L) in aquatic microcosms to establish kinetic parameters for the construction and comparison of two bioaccumulation models, i.e. the biodynamic and saturation models. Cu uptake was recorded in waters exhibiting various concentrations of Na, Mg and Ca at environmental levels to assess the influence of cationic composition on bioaccumulation. Then, the effect of increasing Cu in exposure media on the digestive metabolism of G. pulex was investigated by measuring enzymatic activities (β-glucosidase, N-acetyl-β-glucosaminidase, β-galactosidase). We showed that the saturation model is more suitable than the biodynamic model to describe Cu bioaccumulation in gammarids due to a maximal capacity of animals to accumulate the metal. Cationic composition of water affected insignificantly Cu uptake. All activities of tested enzymes decreased with increasing Cu in exposure media but with different degrees. High correlations were established between the inhibition of enzymatic activities and amounts of Cu bioaccumulated by gammarids. These biological responses could thus provide early-warming of Cu impact on aquatic biota.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

Lebrun

Perret²,

Geffard

et al. 2012

Ecotoxicology

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“…Measurement of metal concentrations in soft tissues was carried out as previously described (Geffard et al 2010) by flameless (Cd, Cu, Ni, and Zn) atomic absorption spectrophotometry with Zeeman correction, using a graphite furnace (SpectrAAZeeman220).…”

Section: Heavy Metal Content In Clamsmentioning

confidence: 99%

The endocrine-disrupting effect and other physiological responses of municipal effluent on the clam Ruditapes decussatus

Mezghani-Chaari

Machreki-Ajmi

Trémolet

et al. 2015

Environ Sci Pollut Res

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In order to document the potential endocrine disrupting and toxic effect of the municipal wastewater effluents discharged into the Sfax coastal area (South of Tunisia), specimens of clam R. decussatus were collected from a reference site and were in vivo exposed to treated sewage effluent for 30 days. To this end, estrogenic and androgenic activities were measured in the gills to assess potential accumulation and regulation of active compounds. After effluent exposure androgenic activity in organic extracts increased up to fivefold compared to controls and remained elevated, while estrogenic activity was not significantly affected by exposure. As a consequence, remarkable disruptions in the gametogenesis activity, glycogen content, and Vitellogenin-like protein levels in male clams were observed. A parallel analysis of heavy metals in clam tissues was determined. A significant uptake of Ni, Zn, and Pb in soft tissues of exposed clams was observed. The significant increase of malondialdehyde (MDA) concentrations as a function of exposure time implies that clams have been exposed to an oxidative stress probably due to the presence of high metal concentrations in sewage effluent. Correlation analysis has revealed a statistically significant and positive relationship between MDA levels and metal concentrations in clams' tissues. The acetylcholinesterase activity was not significantly affected by exposure. Altogether, these results showed that a short-term exposure to a mixture of chemical compounds released by the Sfax wastewater treatment plant induce adverse physiological and reproductive effects in R. decussatus. Further studies are underway in order to evaluate its long-term impacts on aquatic wildlife in the gulf of Gabes area.

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“…However, several studies have reported that addition of AC may have negative impacts on the habitat quality of the benthic organisms, reducing their activity (Janssen and Beckingham 2013;Kupryianchyk et al 2013). On the other hand, many invertebrate species associated with metals and metalloids polluted sediments in aquatic systems and some of them accumulated metals through diet (Geffard et al 2010;King et al 2006;Schaller et al 2011) Therefore, addition of AC and other sorbents to sediment may affect the metal accumulation in organisms. Shen et al (2012) reported that BSAF values for polycyclic aromatic hydrocarbons (PAHs) were elevated when MWNT-2 addition to sediment was greater than 1.5 %.…”

Section: Introductionmentioning

confidence: 99%

Bioturbation effects on heavy metals fluxes from sediment treated with activated carbon

Men

Yang

et al. 2016

Environ Sci Pollut Res

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Adding activated carbon (AC) to sediment has been proposed as an in situ sediment remediation technique. To date, it is not clear whether this technique is effective in the treatment of heavy metal-contaminated sediment in the presence of bioturbators. In the present study, we compare the ability of granular-activated carbon (GAC) and powderactivated carbon (PAC) to reduce Cu, Zn, and Pb pore water concentrations at environmentally relevant concentrations in the absence and presence of Chironomid larvae. Compared to untreated sediment, PAC and GAC addition in the absence of Chironomid larvae resulted in reductions of free Cu concentrations of 78 and 66 % just below the sediment-water interface after 28 days, respectively. While for Pb and Zn these concentration reductions were only 40 and 38, 19 and 25 %, respectively. The presence of Chironomid larvae in untreated, and GAC sediment generally increased the free heavy metals concentrations in pore water, especially in the deeper layers. In comparison with untreated sediment, the coexistence of AC enhanced the accumulation of heavy metals, especially for PAC. This increased bioaccumulation may decrease the survival of Chironomid larvae. The result indicated that ACs may not be suitable for the remediation of heavy metalcontaminated sediments.

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Subcellular compartmentalization of cadmium, nickel, and lead in Gammarus fossarum: Comparison of methods

Cited by 66 publications

References 44 publications

Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

The endocrine-disrupting effect and other physiological responses of municipal effluent on the clam Ruditapes decussatus

Bioturbation effects on heavy metals fluxes from sediment treated with activated carbon

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