1979
DOI: 10.1042/cs0560179
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Subcellular Distribution of Radio-Labelled Iron during Intestinal Absorption in Guinea-Pig Enterocytes with Special Reference to the Mitochondrial Localization of the Iron

Abstract: 1. 59Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5--30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59Fe was extremely rapid and after 5 min 45% of the radioactivity sedimented in the mitochondrial fraction. 4. Density gradient centrifugation indicated that approximately 80% of this radioactivity was associated with the mitochondria themselves; the remainder was in brush-bo… Show more

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Cited by 4 publications
(2 citation statements)
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“…In one species a claim has been made that the transferrin-like protein is unrelated immunologically (12). Low molecular weight iron-binders (13) and mitochondria (14) 30 min or 1 h. Thereafter the loop was removed, washed clean with 40 ml cold saline, and counted for 5"Fe activity in a gamma counter (Beckman Instruments Inc., Fullerton, CA) as a measure of uptake (i.e., adsorption to the mucosa), and the remaining carcass counted (as a measure of absorption into the system) in a small mammal whole body counter against standards contained in a similar volume.…”
Section: Introductionmentioning
confidence: 99%
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“…In one species a claim has been made that the transferrin-like protein is unrelated immunologically (12). Low molecular weight iron-binders (13) and mitochondria (14) 30 min or 1 h. Thereafter the loop was removed, washed clean with 40 ml cold saline, and counted for 5"Fe activity in a gamma counter (Beckman Instruments Inc., Fullerton, CA) as a measure of uptake (i.e., adsorption to the mucosa), and the remaining carcass counted (as a measure of absorption into the system) in a small mammal whole body counter against standards contained in a similar volume.…”
Section: Introductionmentioning
confidence: 99%
“…The cells, resuspended to final volume of 1 ml gave on average, cell counts of 5.5 X 106 cells/ml measured in a hemocytometer, and 5.81±0.90 mg protein as determined by the biuret method (n = 5). (20) and 1.15-1.2 g/ml (14). Assay for the marker enzyme creatine kinase found on the mitochondrial inner membrane on a Centrifuchem System 400 centrifugal analyzer (Roche Laboratories, Nutley, NJ) indicated the presence of mitochondria at 1.18 g/ml d (fractions [27][28].…”
Section: Introductionmentioning
confidence: 99%