2005
DOI: 10.1016/s0076-6879(04)92008-3
|View full text |Cite
|
Sign up to set email alerts
|

Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
4
0

Year Published

2006
2006
2014
2014

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(4 citation statements)
references
References 42 publications
0
4
0
Order By: Relevance
“…However, an alternative method to express short RNA molecules may be required for particular experiments. A need to screen or modify Pol III promoters in order to obtain a strong and reliable inhibition in a particular cell type or organ has been reported (34,35). Occasionally, irreversible silencing of Pol III-dependent transcription was observed in stable expression systems.…”
Section: Discussionmentioning
confidence: 99%
“…However, an alternative method to express short RNA molecules may be required for particular experiments. A need to screen or modify Pol III promoters in order to obtain a strong and reliable inhibition in a particular cell type or organ has been reported (34,35). Occasionally, irreversible silencing of Pol III-dependent transcription was observed in stable expression systems.…”
Section: Discussionmentioning
confidence: 99%
“…Vector-based systems using RNA polymerase III promoters that stably produce siRNA from short hairpin RNA (shRNA) molecules have been established [1][5]. The production of RNAi-mediated gene knockdown transgenic mice (transgenic RNAi mice) has been demonstrated with GFP transgenic mice, which introduced a shRNA expression vector against GFP mRNA (pGtoR) [6], [7].…”
Section: Introductionmentioning
confidence: 99%
“…One method uses modified nucleic acid probes to increase affinity and specificity; such probes include radioactively labeled synthetic RNA oligonucleotide probes, 2 0 -O-methyl RNA oligonucleotides, and locked nucleic acids (LNA). [36][37][38] The most successful method so far uses LNA probes, in which the ribose of the nucleotide contains a bridge connecting its 2 0 oxygen to its 4 0 carbon that locks the ribose in the 3 0 -endo conformation, thereby increasing base stacking forces and backbone preorganization. 37,39 This modification significantly increases the specificity and sensitivity of binding to mature miRNAs.…”
Section: Introductionmentioning
confidence: 99%