Subcellular distribution of β-carotene, retinol, and α-tocopherol in porcine lymphocytes after a single injection of β-carotene

Chew, Boon P.; Wong, Teri S.; Michal, Jennifer J.; Standaert, F. E.; Heirman, L. R.

doi:10.2527/1991.69124892x

Cited by 30 publications

(3 citation statements)

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“…Mitochondria again accounted for 40 to 52% of total β-carotene uptake by blood leukocytes in cats [22] and calves [15], but was lower in dogs with only 14 to 17% [26]. Uptake of β-carotene by blood leukocytes and leukocyte subcellular fractions also has been reported in humans [35], and pigs [36]. The presence of astaxanthin in the various subcellular organelles suggests the involvement of this antioxidant in cellular function.…”

Section: Discussionmentioning

confidence: 94%

Astaxanthin uptake in domestic dogs and cats

et al. 2010

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BackgroundResearch on the uptake and transport of astaxanthin is lacking in most species. We studied the uptake of astaxanthin by plasma, lipoproteins and leukocytes in domestic dogs and cats.MethodsMature female Beagle dogs (18 to 19 mo old; 11 to 14 kg BW) were dosed orally with 0, 0.1, 0.5, 2.5, 10 or 40 mg astaxanthin and blood taken at 0, 3, 6, 9, 12, 18 and 24 h post-administration (n = 8/treatment). Similarly, mature domestic short hair cats (12 mo old; 3 to 3.5 kg body weight) were fed a single dose of 0, 0.02, 0.08, 0.4, 2, 5, or 10 mg astaxanthin and blood taken (n = 8/treatment) at the same interval.ResultsBoth dogs and cats showed similar biokinetic profiles. Maximal astaxanthin concentration in plasma was approximately 0.14 μmol/L in both species, and was observed at 6 h post-dosing. The plasma astaxanthin elimination half-life was 9 to 18 h. Astaxanthin was still detectable by 24 h in both species. In a subsequent study, dogs and cats were fed similar doses of astaxanthin daily for 15 to 16 d and astaxanthin uptake by plasma, lipoproteins, and leukocytes studied. In both species, plasma astaxanthin concentrations generally continued to increase through d 15 or 16 of supplementation. The astaxanthin was mainly associated with high density lipoprotein (HDL). In blood leukocytes, approximately half of the total astaxanthin was found in the mitochondria, with significant amounts also associated with the microsomes and nuclei.ConclusionDogs and cats absorb astaxanthin from the diet. In the blood, the astaxanthin is mainly associated with HDL, and is taken up by blood leukocytes, where it is distributed to all subcellular organelles. Certain aspects of the biokinetic uptake of astaxanthin in dogs and cats are similar to that in humans.

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Section: Discussionmentioning

confidence: 94%

Astaxanthin uptake in domestic dogs and cats

et al. 2010

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“…The present and previous results suggest that retinol directly induces oxidative damage to mitochondria, leading to cyt c release through the opening of mitochondrial PTP. Based on the lipophilic nature of retinoids, the cellular treatment with retinol would lead to an increased intracellular concentration of these compounds, mainly in the mitochondrial fraction (Chew et al, 1991). Further studies should characterize the Fig.…”

Section: Discussionmentioning

confidence: 99%

Vitamin A treatment induces apoptosis through an oxidant‐dependent activation of the mitochondrial pathway

Klamt

Dal‐Pizzol

Gelain

et al. 2008

Cell Biology International

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Even though retinoids are widely used as adjuvant in chemotherapeutic interventions to improve cancer cell death, their mechanism(s) of action involves multiple overlapping pathways that remain unclear. We have previously shown that vitamin A, the natural precursor of the retinoids, induces oxidative-dependent cytochrome c release from isolated mitochondria, suggesting a putative mechanism for apoptosis activation. Using Sertoli cells in culture, we show that retinol causes mitochondrial-dependent apoptosis, involving oxidative stress. Apoptosis was evaluated by nuclear morphology, DNA fragmentation, and caspase-3/7 activity. Retinol induced oxidant- and time-dependent imbalance of several mitochondrial parameters, cytochrome c release and caspase-3/7 activation, leading cells to commit apoptosis. All parameters tested were attenuated or blocked by trolox co-administration, suggesting that retinol induces apoptosis through oxidative damage, which mitochondria plays a pivotal role.

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“…The antioxidant role of carotenoids accumulated in skin plays a vital role against these ROS [8][9][10]. The antioxidant role of beta-carotene has been extensively studied and has been widely reported [11][12][13][14][15]. Similarly, beta-carotene (a precursor of vitamin A) also plays an important role in vision, cell-differentiation, synthesis of glycoproteins, etc [16].…”

Section: Introductionmentioning

confidence: 99%

Time-temperature dependent variations in beta-carotene contents in carrot using different spectrophotometric techniques

Ullah¹,

Khan²,

Shah³

et al. 2018

Laser Phys.

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The current study presents time dependent variations in the concentration of beta-carotene in carrot under different storage-temperature conditions using UV-VIS and Raman spectrophotometric techniques. The UV-VIS absorption spectra of beta-carotene extracted from carrot shows three distinct absorption peaks at 442, 467, and 500 nm with maximum absorption at 467 nm. These absorption peaks are very much reproducible and are assigned to β-carotene. Similarly, Raman spectra of carrot samples also confirmed the three main Raman peaks of beta-carotene at shift positions 1003, 1150, and 1515 cm −1 . An overall decrease in beta-carotene content has been observed for time-temperature conditions. These results depict a decrease of about 40% in the content of beta-carotene when carrot samples were stored in a refrigerator (4 °C) for the first 20 d, whereas a decrease of about 25% was observed when carrot samples were stored in a freezer (−16 °C) for the same period. The objective of this study is to investigate the possible use of Raman spectroscopy and UV-VIS spectroscopy for quick and detailed analysis of changes (degradation) in beta-carotene content associated with time and temperature in storage (frozen foods) in order to promote quality foods for consumers. Future study with a greater focus on the concentration/content of beta-carotene in other fruits/vegetables is also desirable.

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Subcellular distribution of β-carotene, retinol, and α-tocopherol in porcine lymphocytes after a single injection of β-carotene

Cited by 30 publications

References 0 publications

Astaxanthin uptake in domestic dogs and cats

Astaxanthin uptake in domestic dogs and cats

Vitamin A treatment induces apoptosis through an oxidant‐dependent activation of the mitochondrial pathway

Time-temperature dependent variations in beta-carotene contents in carrot using different spectrophotometric techniques

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